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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
91

Advanced glycation end product precursors in diabetes : a crucial link between oxidative stress and inflammation?

Le Brocq, Michelle Louise January 2010 (has links)
Advanced glycated end-products (AGEs) are a heterogenous group of compounds formed through the Maillard reaction. During AGE formation, reactive α-dicarbonyls are formed, such as glyoxal (GO) and methylglyoxal (MG). These α-dicarbonyls are present at elevated concentrations in diabetes, and are frequently implicated in the initiation and progression of diabetic complications. Previous research has linked α-dicarbonyls with formation of reactive oxygen species (ROS) and inflammation. However, much of the prior work has been conducted using concentrations of α-dicarbonyls that are substantially higher than can be generated in vivo, and the biochemistry has been investigated under conditions (e.g. pH) outside the physiological range. The aim of the work presented in this thesis was to test the hypothesis that GO and MG are pro-oxidant and pro-inflammatory at (patho)physiological concentrations in both monocytes and pancreatic β-cells. In this work several new and important observations have been made regarding the action of α-dicarbonyls on oxidative stress and inflammation. 1) The amount of oxidative species production by α-dicarbonyls in glycation reactions with amino acids and proteins may be so low as to be negligible in vivo, despite previous evidence to the contrary. 2) α-dicarbonyls did not appear to generate oxidative stress within inflammatory cells nor pancreatic β-cells by depleting the levels of GSH. 3) At least in the β-cell model, the mechanism of action of the α-dicarbonyls did not involve dysregulation of the antioxidant SOD enzymes. 4) Neither α-dicarbonyl significantly affected insulin production by β-cells, except at cytotoxic concentrations. 5) Treatment of inflammatory cells with α-dicarbonyls induced release of the proinflammatory cytokine IL-8. 6) In both immune cells and pancreatic β-cells, α-dicarbonyls were involved in O2.- generation by activation and/or upregulation of NADPH oxidase. 7) Despite the structural similarities of α-dicarbonyls, they have distinct mechanisms of action with respect to oxidative stress.
92

Studies on the native glycoprotein of the sheep colonic mucosa

Taylor, Doris Jean January 1970 (has links)
No description available.
93

Visualization and characterization of cell surface glycoproteins on mouse neuroblastoma cells

Maher, Pamela A. January 1980 (has links)
The cell surface glycoproteins of mouse neuroblastoma cells were visualized using fluorescent lectins in combination with fluorescent light microscopy and SDS-gel electrophoresis, lectin-microsphere conjugates in combination with scanning electron microscopy, and iodinated lectins. The lectins used in these studies were: Con-canavalin A (ConA) which is specific for α-D-mannose and α-D-glucose groups, wheat germ agglutinin (WGA) which binds to N-acetylglucosa-mine oligomers and nicinus communis agglutinin (RCAI) which is specific for D-galactose residues. All three lectins were found to have over 10⁷ high affinity (K[sub d]= 2 x 10⁻⁷ M) binding sites on the neuroblastoma cell surface. These sites were found to be densely and randomly distributed over the surfaces of cells which were fixed with glutaraldehyde before labelling or labeled at 4°C. However, when the cells were labeled at 23°C or 37°C, the lectin receptors were found to undergo redistribution. All three lectins were internalized by the cells in an energy- dependent process when the cells were treated with the lectins for 60 min or more at 37°C. However, the patterns of redistribution for the different lectin receptors were not the same. When cells were labeled with ConA, the receptors were shown to patch and then clear from the surface of the cells. Once the label had completely cleared from the cell surface, the cells could not be labeled with additional ConA, even when the first labelling was done using ConA at concentrations well below saturation. However, when cells were treated in the same way with WGA or RCAI, a heavy, uniform display of marker was seen on the cell surface at all times. It was only when the cells were briefly labeled with these lectins and then incubated in buffer that it became apparent that these receptors could also patch and clear from the cell surface. In addition, in order to clear all the receptors for these two lectins from the cell surface, it was necessary to label the cells with saturating concentrations of lectin. Thus, labeled and unlabeled ConA receptors on the neuroblastoma cells undergo co-ordinate redistribution whereas labeled WGA and RCAI receptors redistribute independently of their unlabeled counterparts. Studies with drugs which disrupt the various cytoskeletal elements suggested that the microfilament system played a role in the coordinate redistribution of ConA receptors. Double labelling studies with both different iodinated and fluorescent lectins indicated that the binding sites for WGA were not associated with those for ConA. WGA binding sites did appear to be directly associated with many of the binding sites for RCAI. Studies with ConA and RCAI were not carried out because it was shown that ConA binds to RCAI. Using the fluorescent lectins in combination with SDS-gel electrophoresis, the lectin-binding polypeptides from the neuroblastoma cell membranes were identified and characterized. Plasma membranes were purified from the neuroblastoma cells using hypotonic disruption followed by differential and isopycnic gradient centifugation. The membrane preparation showed a 10-fold increase in the specific activity of two plasma membrane markers and little contamination by other cell components. The membranes were dissociated in SDS and run on SDS-polyacrylamide gels to separate out the different polypeptides. The gels were fixed and stained with fluorescent lectins. WGA and RCAI were both found to bind almost exclusively to a single polypeptide with a molecular weight of 30,000 daltons. This polypeptide also stained strongly for carbohydrate after periodate oxidation. ConA, on the other hand, bound to over 20 polypeptides, most of which had molecular weights between 60,000 and 120,000 daltons. However, when the cells were made to internalize all their ConA receptors and their membranes isolated and run on gels, four of the ConA-staining polypeptides were found to be absent as compared to membranes from untreated cells. Thus, it appears that only four of the ConA-binding polypeptides seen on the gels were available for ConA binding at the cell sur- face. These polypeptides also labeled with I¹²⁵ when intact cells were treated with I¹²⁵ and lactoperoxidase. / Medicine, Faculty of / Biochemistry and Molecular Biology, Department of / Graduate
94

Purification of glycoproteins from herpes simplex virus

Graham, Richard Peter 25 July 2017 (has links)
The aim of this work was to purify type-specific glycoproteins from herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) for diagnostic use. The most likely candidate for a type-specific glycoprotein of HSV-1 is glycoprotein C (gC), although it has recently been shown to contain some type-common antigenic determinants. HSV-1 and HSV-2 were produced in BHK-21 cells and labelled with either (³H)-glucosamine ((³H)-gln) or a mixture of (¹⁴C)-amino acids ((¹⁴C)-aa). Analysis of the radiolabelled products by analytical sodium dodecyl sulphate polyacrylamide gel electrophoresis (SOS-PAGE) and autoradiography revealed that in the HSV-1 infected cells the radiolabelled components were incorporated into viral specific proteins only, whereas in the HSV-2 infected cells they were incorporated into host cell proteins as well as viral proteins. Preparative polyacrylamide gel electrophoresis (Prep-PAGE) was used as an initial step in separating HSV-1 infected cell proteins labelled with (³H)-gln. Two cycles of Prep-PAGE were sufficient to produce solutions containing either glycoprotein B ( gB) or glyco- protein C (gC), which were free of other HSV-1 glycoproteins. However, these solutions still contained a number of non-glycosylated proteins. Two different techniques were utilized to remove the non-glycosylated proteins from the glycoprotein solutions. Hydroxylapatite (HAUltrogel) chromatography in the presence of sodium dodecyl sulphate (SDS-HTP) did not separate the different HSV-1 glycoproteins and was not satisfactory for removing the non-glycosylated proteins. Gel-bound lectin affinity chromatography using wheat germ lectin and Helix pomatia lectin was not successful in purifying the glycoproteins because the glycoproteins which bound to the lectins could not be eluted under normal conditions. Difficulties encountered in eluting the HSV-1 glycoproteins from the lectins may have been due to the sodium dodecyl sulphate (SOS) in which the proteins were solubilized. For this reason, the gelbound lectin affinity chromatography was repeated using HSV-1 membrane proteins solubilized in a non-ionic detergent, Triton X-100. Using material prepared in this way, several HSV-1 glycoproteins were bound by wheat germ lectin and eluted under normal conditions to yield glycoproteins which were purified with respect to nonglycosylated proteins.
95

Purification and characterization of cardiac and skeletal muscle calsequestrin using phenyl-sepharose

Cala, Steven Edward January 1983 (has links)
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
96

The interaction of the glycoprotein folding sensor, UDP-glucose:glycoprotein glucosyltransferase, with glycoprotein substrates /

Taylor, Sean Caldwell January 2002 (has links)
No description available.
97

The role of dystroglycan in the formation of the neuromuscular junction /

Jacobson, Christian B. January 2000 (has links)
No description available.
98

Modulation of endothelial cell survival by the angiopoietin-1Tie-2 receptor pathway

Harfouche, Rania. January 2002 (has links)
No description available.
99

Studies on the glycoproteins of porcine, avian and bovine plasmas

Grant, Donald Lloyd January 1966 (has links)
No description available.
100

Comparison of glycoproteins of blood from fish and mammals.

Qureshi, Mohiy-Ud-Din. January 1970 (has links)
No description available.

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