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This dissertation presents the development of an alternative aptamer screening process, Competition-Induced Selection of Ligands (CISL), and its use in screening for ssDNA aptamers for gold substrates. Gold substrates are presented as the nonnucleotide target for implementing CISL as a novel aptamer screening approach. Chapter 1 provides an overview of the in vitro selection of oligonucleotide aptamers, the polymerase chain reaction that is a key step in the aptamer screening process, the synthesis and properties of gold nanoparticles and the biomolecule-mediated formation of inorganic nanoparticles. Chapter 2 presents the goals and objectives of this thesis along with an organizational overview of the dissertation. Chapter 3 describes the experimental techniques and optimizations pertinent to the development of the CISL aptamer screening process. Chapter 4 investigates the effects of various nucleic acid additions during the seed-mediated growth of gold nanoparticles. Chapter 5 discusses the use of CISL in screening for ssDNA aptamer candidates for spherical gold nanoparticles (AuNPs) and the primary and secondary structure analysis of identified sequences. Chapter 6 presents the use of CISL in screening for ssDNA aptamer candidates for planar gold substrates (PlanarAu) and also includes primary and secondary structure analysis of identified sequences accompanied with an incubation study to provide a “frequency” ranking of aptamers as adsorbate species on PlanarAu. Chapter 7 offers concluding remarks and ideas for future expansion and applications of this work.

published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy

M.Com. / Please refer to full text to view abstract

Gold mining is often associated with positive economic benefits; however, it may also have negative impacts on the environment and human health. It is essential that communities understand the risks and benefits associated with mining, particularly in developing countries where there is often a lack of legislation governing the environmental performance of mining corporations. The perceptions of local people regarding the risks and benefits of mining may differ significantly from those of company representatives, policy makers, and the scientific community. Indeed, public concerns have sometimes been ignored or downplayed by those responsible for the implementation of environmental policies and practices. Examination of the complex social, economic, psychological, political, and cultural factors influencing risk perception in mining communities is thus important for successful risk communication and management. Issues arising from the interplay between socio-economic benefits and risks are particularly acute in small island states which have isola ted and highly limited economic development trajectories. The lessons learned in such cases are therefore instructive for crisis planning across the developing world. This study conducted firsthand empirical research into the perception of environmental and health risks in the communities surrounding the Vatukoula gold mine in Fiji. Primary data was obtained through a survey questionnaire designed to quantify and evaluate perceived risks (n= 340, representing approximately 24% of the target population). Concurrently, environmental samples were collected to assess the extent of environmental impacts at the study site. Study results revealed that gender is an important variable in risk perception at Vatukoula. Major findings include: (1) women feel they have less knowledge about the risks of mining compared to men; (2) women feel they have less control to avoid the risks of mining compared to men; and (3) women and men tend to receive risk messages from different sources. The information obtained during this study was made directly available to local stakeholders, to aid in risk management and decision-making.

Thesis--University of Turku. / At head of title: From the Department of Prosthetics, Institute of Dentistry, University of Turku, Finland. Translated by Mr. and Mrs. Antony Landon. Includes bibliographical references (p. 91-96).

Thèse--Nancy. / At head of title on added t.p.: Gouvernement général de l'Afrique occidentale française. Service géologique. "Bibliographie": p. 12-16.

Thèse--Nancy. / At head of title on added t.p.: Gouvernement général de l'Afrique occidentale française. Service géologique. "Bibliographie": p. 12-16.

Thesis (Doctoral)--Ludwig-Maximilians-Universität zu München, 1935.

Thesis (Doctoral)--Ludwig-Maximilians-Universität zu München, 1935.