Nowadays, field development strategy has become increasingly dependent on the results of reservoir simulation models. Reservoir studies demand fast and efficient results to make investment decisions that require a reasonable trade off between accuracy and simulation time. One of the suitable options to fulfill this requirement is streamline reservoir simulation technology, which has become very popular in the last few years. Streamline (SL) simulation provides an attractive alternative to conventional reservoir simulation because SL offers high computational efficiency and minimizes numerical diffusion and grid orientation effects. However, streamline methods have weaknesses incorporating complex physical processes and can also suffer numerical accuracy problems. The main objective of this research is to evaluate the numerical accuracy of the latest SL technology, and examine the influence of different factors that may impact the solution of SL simulation models. An extensive number of numerical experiments based on sensitivity analysis were performed to determine the effects of various influential elements on the stability and results of the solution. Those experiments were applied to various models to identify the impact of factors such as mobility ratios, mapping of saturation methods, number of streamlines, time step sizes, and gravity effects. This study provides a detailed investigation of some fundamental issues that are currently unresolved in streamline simulation.