Green space fragmentation is one reason for the failing ecological networks around our planet. It has been argued that the driving force behind the loss of natural habitats by fragmentation is urban development. This essay aims to look at the subject of green space fragmentation in Uppsala municipality and how it affects both humans and roe deer living in the area. This essay will also explore how the efforts to stop wildlife-vehicle collisions impact roe deer’s ability to roam through the area divided thanks to human induced green space fragmentation. This has been done by using a descriptive quantitative analysis combined with a connectivity model, the least-cost approach. Remote sensing was used to collect the data used for the analysis. The results of this essay indicated that green space fragmentation impacts both humans and roe deer in Uppsala municipality since the wildlife-vehicle collisions can be traced to spots where the roe deer are forced to cross the road because of green space fragmentation. The introduced form of wildlife crossing structures, wildlife fences, does not help minimize the effects of the fragmentation, instead it steers the animals into certain points where the collisions occur. To truly minimize the consequences of human induced green space fragmentation, green infrastructure needs to be taken into account whenever planning future developments.