This study utilized GIS and statistical methods to map the spatial variability of nitrate and related groundwater constituents in 30 counties above the Seymour Aquifer, analyze temporal patterns of nitrate pollution, identify probable sources of pollution, and recommend water development strategies to minimize exposure to nitrate and reduce future aquifer contamination. Nitrate concentrations in excess of 44 mg/L (US EPA limit) were commonly observed in the Seymour Aquifer region, especially in the central agricultural belt. Data indicated that this is an ongoing problem in the Seymour Aquifer and that agricultural activity and rural septic systems are the likely sources of the nitrate. Inconclusive results emphasized the need for a more comprehensive spatial and temporal water quality monitoring.