This research examines the relationship between growth, growth disruption, and skeletal indicators stress amongst juveniles (n=60) interred at the Late Antique infant and child cemetery at Poggio Gramignano (PG) (ca. 5th century CE), associated with a rural agricultural community. Growth disruption – evidenced by decreased long bone length compared to dental age – and stress experience – evidenced by skeletal stress indicators – were compared to those within juveniles from two urban Roman-era cemeteries (n=66), Villa Rustica (VR) (0-400 CE) and Tragurium City Necropolis (TCN) (400-700 CE). Results indicate that the PG juveniles had significantly smaller femoral lengths-for-age than VR and TCN; however, the frequency of skeletal stress indicators were higher among juveniles at VR and TCN. These differences in growth and stress experience likely resulted from differing biosocial and ecological environments present in the different regions at the time, including differing nutrition, disease, and socioeconomic structures between urban and rural Roman populations.