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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Studies towards the total synthesis of (-)-gymnodimine

Wang, Guoqiang 30 November 2005 (has links)
Studies towards the total synthesis of (-)-gymnodimine (1), a marine neurotoxin with a unique molecular architecture and pronounced biological activity, are described. These studies resulted in a convergent approach to the advanced intermediate 315, containing the C3-C32 section of 1. Two synthetic routes were developed to construct the tetrahydrofuran subunit of (-)-gymnodimine. Both routes employed a highly stereoselective iodoetherification of alkenes 120 (initial route) and 158 (second generation route) to furnish cis-2,5- disubstituted tetrahydrofurans 121 and 159, respectively. The longest sequence in the second generation route required 16 linear steps to the tetrahydrofuran subunits. The initial approach needed 21 steps. Two cyclohexene subunits of (-)-gymnodimine, 246 and 265, were prepared from the Diels-Alder cycloadducts 213 and 214, respectively. The quaternary stereogenic center at C22 (gymnodimine numbering) of 236 was generated by the intramolecular lactonization of 235, which was obtained from 213. Assembly of two subunits, 146 and 299, employing a B-alkyl Suzuki-Miyaura reaction provided the coupled product 310 in good yield. After 310 was converted to the terminal alkyne 313, methylation of 313 was accomplished via a Stille reaction. In addition, a model study of the formation of the cyclic imine moiety of (-)-gymnodimine was carried out. / Graduation date: 2006
2

Proteomic and physiological studies of paralytic shellfish toxin producing dinoflagellates Alexandrium tamarense and Gymnodinium catenatum /

Chiu, Ellen. January 2006 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2007. / Title proper from title frame. Also available in printed format.
3

Proteomic and physiological studies of paralytic shellfish toxin producing dinoflagellates: Alexandriumtamarense and Gymnodinium catenatum

Chiu, Ellen., 招雅莉. January 2006 (has links)
published_or_final_version / abstract / Ecology and Biodiversity / Master / Master of Philosophy
4

Detection And Quantification Of Karenia Brevis By Carbon Fixation Gene Expression Analysis

Gray, Michael Alan, 04 March 2004 (has links)
Karenia brevis (Davis cf. Hansen & Moestrup = Gymnodinium breve) is the non-peridinin containing dinoflagellate responsible for many harmful algal blooms (red tides) in the Gulf of Mexico. These recurrent blooms can have significant negative ecological, economic, and human health impacts including fish kills, tainting of shellfish, poisoning of marine mammals, loss of tourism revenue due to beach closures, and respiratory distress and food poisoning in humans. A method for detection of Karenia brevis was developed based upon amplification of the mRNA for the plastid-encoded gene of the carbon fixing enzyme ribulose 1, 5-bisphosphate carboxylase/oxygenase (RuBisCO) large subunit (rbcL). Using sequence information from a primer set targeting a 554-bp region of the Karenia rbcL gene, a small (91 bp amplicon) primer and probe set was created for TaqMan(registered trademark) real time RT-PCR of K. brevis rbcL. The primer/probe set is sensitive to as little as 0.1 fg of target transcript and as little as 1 pg of total cellular K. brevis RNA extract, corresponding to less than 1 cell reaction-1. The primer/probe set did not amplify rbcL transcript from any of the non-target algae tested. Bloom samples analyzed by this method have shown the assay to be a reliable method, with effective enumeration and a linear relationship showing good correlation to the cell counts by microscopy (r2= 0.8344). The assay has been shown to be robust and perform well even in non-ideal conditions, with pre-extraction RNA from unialgal culture stable at room temperature for up to 3 days and up to a month at -80 degrees C in Stratagene's lysis buffer. The transcription of the rbcL gene demonstrated minor variation throughout the diel period, however the variation was not linked to the diel cycle or to carbon fixation, which showed a distinct diel signal. Due to the relatively constant expression of the rbcL gene, the real-time RT-PCR assay developed should be able to reliably enumerate K. brevis populations in the natural environment, as long as the sample is placed in Stratagene's lysis buffer and processed within one or two days or frozen at -80 degrees C and processed within a month.

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