DAUGIAMEČIŲ ŽOLINIŲ AUGALŲ POPULIACIJŲ VERTINIMO HERBOCHRONOLOGIJOS METODAIS GALIMYBĖS SANTRAUKA Magistriniame darbe pateikiami gausialapio lubino (Lupinus polyphyllus), aukštosios dedešvos (Malva alcea), valgomosios rūgštynės (Rumex acetosa), tankiažiedės rūgštynės (R. confertus), bukalapės rūgštynės (R. obtusifolius), paprastosios varnalėšos (Arctium tomentosum), paprastosios trūkažolės (Cichorium intybus), Sosnovskio barščio (Heracleum sosnowskyi), valgomojo krieno (Armoracia rusticana) metinių rievių išsidėstymo šaknyse aprašymai. Išsamiai išnagrinėtos trys skirtingos gausialapio lubino (Lupinus polyphyllus) populiacijos, įvertinant koreliacijas tarp augalo amžiaus ir kitų morfometrinių parametrų: vegetatyvinių ūglių skaičiaus, generatyvinių ūglių ilgio ir skaičiaus, žiedyno ilgio, ankščių ir subrandintų sėklų skaičiaus. Gausialapis lubinas (L. polyphyllus) išsamiems herbochronologiniams tyrimams pasirinktas dėl spartaus plitimo natūraliose ir suardytose buveinėse, didelės antžeminės biomasės, didelio konkurencingumo. Herbochrologiniais metodais išanalizuotos trys skirtingos gausialapio lubino (L. polyphyllus) populiacijos, jose nustatytos silpnos koreliacijos tarp augalų amžiaus ir tirtų antžeminių dalių. Tiek skirtingose populiacijose augę augalai, tiek tų pačių populiacijų vienodo amžiaus individai labai skiriasi pagal išaugintą vegetatyvinių ir generatyvinių ūglių skaičių, subrandintą sėklų kiekį. Šie tyrimai parodė, kad vertinant augalus vien pagal morfologinius... [toliau žr. visą tekstą] / POSSIBILITIES OF EVALUATION OF PERENNIAL HERBACEOUS PLANT POPULATIONS APPLYING HERBOCHRONOLOGICAL METHODS SUMMARY Arrangement of annual growth rings in the roots of Lupinus polyphyllus, Malva alcea, Rumex acetosa, R. confertus, R. obtusifolius, Arctium tomentosum, Cichorium intybus, Heracleum sosnowskyi, Armoracia rusticanais were analysed. Three different populations of Lupinus polyphyllus were analysed thoroughly and evaluation of correlation between the age of the plant and other morphometric parameters (the number of vegetative sprouts, the length and number of generative sprouts, the length of the raceme and the number of pods and ripened seeds) is presented. L. polyphyllus has been chosen for thorough herbochronological analysis because of its invasiveness into natural and disturbed habitats, large aboveground biomass and great ability to survive. Using herbochronological methods three different populations of L. polyphyllus have been analysed and weak correlation between the age of the plants and investigated aboveground parts has been discovered. Both the plants growing in different populations and the individuals of the same age growing in the same population are very different by the number of formed vegetative and generative sprouts and ripened seeds. This research has shown that determination of the age of a plant by morphological indications only is complicated. It should be taken into account that the growth conditions could influence the growth and formation of... [to full text]

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There is a great deal of vegetation literatures about vegetation of forest and alpine grass in Taiwan, but the weed vegetation survey often investigates the species only. As the study of vegetation in Taiwan is for away from completeness, and weed is important to biodiversity is imperative, therefore the study of weed vegetation. The study selected weed vegetation in Daliau Township as study area, and surveyed floristic composition and environmental factors. The study used Branu-Blanquet method, and set up 304 plots. Classification of the vegetation types was conducted by cluster analysis and tabular comparison method, and following the treatment of Taiwan vegetation classification system. Detrended correspondence analysis (DCA) and nonmetric multidimentional analysis (NMDS) used to clarify the relationship between vegetation composition and environmental factors. The lower two levels of the hierarchy, alliance and association, were based on floristic composition according to the floristic-sociological concept of diagnostic species. The relationships of floristic composition and environmental factors were not significant, and this might be caused by that the composition of weed communities can quickly responsed to weather and human disturbances. The resultant system consisted of 2 classes, 3 formations, 21 alliances, 37 associations, 6 tentative vegetation types and 10 intermediate vegetation types. It may supply some misgiving of study of vegetation in Taiwan.

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Container grown perennials are a popular product offered by nurseries and greenhouses and included in their production but little research has been reported on proper overwintering techniques for herbaceous perennials and ornamental grasses. In the first experiment rooted liners of Pennisetum alopecuroides, Pennisetum "Hameln" and Pennisetum "Little Bunny," were potted. Treatments included, utilizing two overwintering covers, two fertilizer rates (low or high) and two substrate moisture contents (wet or dry). Covering with either a double layer of Dewitt N-Sulate™ insulation fabric or a double layer of Dewitt N-Sulate™ insulation fabric and a single sheet of 4 mil white polyethylene plastic on top of the insulation fabric, wet substrate treatments, low fertility rates and combinations of both, improved survival and vigor for all three tested Pennisetum species and cultivars. In the second experiment, rooted liners of P. alopecuroides, P. "Hameln" and P. "Little Bunny," were potted. Treatments included, two transplanting times (young or old), two fertilizer rates (low or high) and two substrate moisture contents (wet or dry) at the UHC and Poplar Ridge Nursery (Montross, VA) (PR). Vigor was improved for P. alopecuroides (at PR) and P. "Little Bunny" (at both locations) with the young transplanting time. In the third experiment, older plant material of P. alopecuroides, P. "Hameln" and P. "Little Bunny," were subjected to fertility treatments of no additional fertilizer or top-dressed at a low, medium or high rate . A high fertility rate reduced survival and vigor for P. "Little Bunny." In the fourth experiment rooted liners of Echinacea purpurea "Hot Papaya," Echinacea purpurea "Milkshake," Gaillardia x grandiflora "Gallo Peach," Heuchera x villosa "Pistache," Heuchera x villosa "Brownies," P. alopecuroides, P. "Cassian," P. "Hameln" and P. "Little Bunny," were potted and overwintered at the UHC or Poplar Ridge Nursery (Montross, VA) (PR) or Riverbend Nursery, Inc. (Riner, VA) (RB). Treatments included, utilizing two overwintering covers, two fertilizer rates (low or high) and two substrate moisture contents (wet or dry). Vigor at the UHC, was reduced with the high fertility rate for E. "Hot Papaya" and H. "Brownies." A double layer of Dewitt N-Sulate™ insulation fabric and white polyethylene plastic on top of the cover, in combination with the wet substrate moisture treatment improved vigor of E. "Hot Papaya."A double layer of Dewitt N-Sulate™ insulation fabric in combination with the wet substrate moisture content and the high fertility rate reduced P. "Cassian" vigor. No overwintering cover reduced P. "Hameln" vigor. No overwintering cover and the high fertility rate reduced P. "Little Bunny" vigor. Vigor at PR was improved with the high fertility rate for E. "Milkshake," G. "Gallo Peach" and H. "Brownies." At RB, a double layer of Dewitt N-Sulate™ insulation fabric in combination with the low fertility treatment and no cover in combination with the high fertility treatment reduced vigor for E. "Milkshake" and P. "Little Bunny," respectively. No cover in combination with the wet substrate moisture treatment reduced vigor for G."Gallo Peach." / Master of Science

New hybrid Echinacea cultivars, based on crosses of Echinacea purpurea (L.) Moench with several other Echinacea species, have generated interest and excitement in the marketplace due to novel flower colors and forms. However, these cultivars vary significantly in their growth habits and requirements from the species. We examined factors in the production of Echinacea hybrid cultivars to provide guidance to growers. Foliar sprays 600 mg·L⁻¹ benzyladenine (BA) increased numbers of branches between 19% and 83% in Echinacea cultivars while 400 mg·L⁻¹ dikegulac sodium or 500 mg·L⁻¹ ethephon did not improve branching. Of several height control PGRs applied to E. ‘Marmalade,’ only plants treated with two applications of 5000 mg·L⁻¹ daminozide were shorter (24%) compared to untreated controls although flowering was also reduced by 70%. Echinacea ‘Harvest Moon’ plants were shorter in response to all of the PGRs applied, with the best results seen in plants treated with foliar sprays of uniconazole (one application of 30 mg·L⁻¹ or two applications of 15 mg·L⁻¹ ), two applications of 5000 mg·L⁻¹ daminozide, or 4 mg·L⁻¹ paclobutrazol applied once as a drench. Supplying N at 150 mg·L -1 during the growing season provided Echinacea cultivars adequate nutrition and maximized numbers of branches and flowers and shoot dry weight. In overwintering, fertilization treatments that resulted in low substrate electrical conductivity going into dormancy, 5.0 kg·m controlled release fertilizer 15N-3.9P-10K or 150 mg·L⁻¹ N using 15N-2.2P-12.5K applied using constant liquid feed, resulted in the highest survival rates of Echinacea cultivars. As a monitoring tool, SPAD measurements were not successful in predicting tissue N levels in Echinacea hybrids. Twenty-one hybrid cultivars acquired as stage 3 tissue culture plantlets were grown under one of three photoperiods (10-hour, 16-hour, or 24-hour) for 10 weeks before being transplanted to larger containers and grown under natural daylength until flowering. Providing Echinacea hybrid cultivars with a 16-hour photoperiod during liner production resulted in plants which flowered soonest without negative effects on growth. The need for height control PGRs varied by cultivar; however, overall height control PGRs controlled flower stalk height and increased market rating. / Ph. D.

Herbaceous peonies (Paeonia lactiflora Pall.) are common perennials used both in gardens and the landscape as well as for cut flowers. Peonies require a chilling period to break dormancy but not for flower bud differentiation. For all studies discussed in this dissertation, two peony cultivars, Sarah Bernhardt and Inspecteur Lavergne, small (3–5 eye) crowns from Holland were potted in 3.8-L pots in mid-November of 2017 and 2018. Our overall objective was to determine if we could manipulate chilling time, along with application of gibberellic acid (GA3) and growth retardants, to produce marketable containerized peonies from a small crown in a single season (November to May). We evaluated chilling, GA3 and a growth retardant (uniconazole; UNZ) under controlled chilling and greenhouse forcing conditions. All potted plants were held outdoors at Battlefield Farms (Rapidan, VA, 38˚ N) for 4 weeks [in 2017, 400 chilling units (CU) according to Fulton Chilling Model] or in a 10°C cooler for 5.5 weeks (in 2018, 400 CU) to root, then placed in a 5°C cooler for 3, 4 or 5 weeks (total 752, 869 or 986 CU). GA3 was applied as a 0 or 100 mg·L-1 drench at 250 ml/pot after the plants were moved into the Virginia Tech greenhouse (Blacksburg, VA, 37˚ N) for forcing. Uniconazole drenches were applied to each cultivar under each chilling treatment at 355 ml/pot at 0, 15, or 20 mg·L-1 at 7 days after the GA3 drench applications. Three weeks chilling at 5°C (752 CU total) provided sufficient chilling for 'Sarah Bernhardt' and 'Inspecteur Lavergne'. Application of GA3 reduced production time and resulted in a greater number of shoots, and, in three of the four studies, increased the number of flowering shoots in three of the four studies. Substrate drench application of 15 mg·L-1 UNZ prior to spring emergence reduced plant width moderately resulting in improved compactness of both cultivars. We evaluated the effects of plant growth retardants applied with different methods at different stages of production on the growth and development of containerized peony under nursery conditions. All potted plants were placed in an unheated coldframe at the Virginia Tech Urban Horticulture Center (Blacksburg, VA, 37˚ N) for one month after potting to promote rooting and then were moved outdoors to a gravel pad to receive natural chilling from November to February. In 2017–18, substrate drenches of UNZ at 0, 15, 30 or 45 mg·L-1 or paclobutrazol (PBZ) at 0, 30, 60 or 90 mg·L-1 at 237 mL/pot were applied about 4 weeks after potting for both cultivars in mid-December 2017. In 2018–19, fall drenches of uniconazole at 0, 15, 30 or 45 mg·L-1 at 237 mL/pot were applied about 4 weeks after potting in mid-December 2018, or spring sprenches of uniconazole were applied at 0, 15, 30 or 45 mg·L-1 at 840 mL·m-2 in March 2019 after 50% shoot emergence for each cultivar. Plant growth retardant applications had little effect on plant growth of either cultivar, but treated plants were of a darker green color compared to the control plants. In addition, higher rates of uniconazole applied as a fall drench increased the number of flowering shoots of both cultivars and the percentage of plants flowering for 'Sarah Bernhardt' in the second season of the study where plants were more protected from spring freezes. Fall paclobutrazol drenches or spring uniconazole sprenches had little effect on flowering. To determine the best timing for spring GA3 applications under nursery conditions, we applied three models based on natural chilling accumulation. The models were a modified Fulton Chilling Model (FCM) for herbaceous peonies, Blackberry Chilling Model 5 (BCM5) for blackberry, or a visual development model (VDM) which was 10% of plants showing shoot emergence in the spring. We choose 1,000 CU for the first two chilling models as the chilling required to break dormancy and promote normal plant growth and flowering. All plants were held in an unheated coldframe at the Virginia Tech Urban Horticulture Center for one month after potting to promote rooting, then were moved outdoors to a gravel pad to receive natural chilling over the winter months. Drenches of 0 or 100 mg·L-1 GA3 were applied at 250 mL/pot to each cultivar under each chilling model when the specific conditions were met. Due to greater winter injury in the 2017–18 season, results varied by year. In the 2017–18 season, GA3 applied according to BCM5 reduce days to emergence for both cultivars and reduce the plant width of 'Inspecteur Lavergne', and later application according to BCM5 and VDM reduced plant length and diameter of 'Sarah Bernhardt'. Reductions in plant size may have been due to greater winter injury due to the earlier emergence of GA3 treated plants. In the 2018–19 season, earlier GA3 drench applications tended to reduce days to emergence for both cultivars and the FCM application reduced days to bud for 'Inspecteur Lavergne', but GA3 drench applications had no effect on plant size. GA3 can be applied after chilling (1,000 CU) using a suitable chilling model such as FCM for peonies, or BCM5, or VDM, but GA3 had little effect on plant development under nursery conditions. We also evaluated GA3 effects on peony bud differentiation and development during controlled chilling and early forcing, as well as effects on growth and flowering. All potted plants were held in a 10°C cooler for 5.5 weeks (400 CU) to root, then placed in a 5°C cooler for 4 weeks (total 869 CU). GA3 was applied at 0 or 100 mg·L-1 pre-chilling or post-chilling as a 250 ml/pot drench. Bud differentiation and development of excised buds were evaluated using a stereomicroscope at potting, after rooting (before chilling), after 1, 2, 3 or 4 weeks of chilling, and at 5, 10 or 15 days after the beginning of forcing. All buds were removed from the sample plants, measured for bud length and diameter, and dissected under a stereomicroscope to assess differentiation stages. Root dry weights and crown dry weights were also determined after rooting, after chilling, and at 15 days of forcing. Ten plants of each treatment were grown in the Virginia Tech greenhouse after chilling until flowering. GA3 applications did not advance the bud development stage because most of buds were already in the reproductive stages before dormancy, but GA3 enhanced bud elongation during chilling and the early forcing period. Our findings suggest that GA3 applications can reduce the time to emergence and flowering, as well as increase the numbers of shoots and flowering shoots. GA3 applied right after rooting in, prior to the chilling period, or before greenhouse forcing, resulted in earlier emergence and flowering with higher quality plants. However, earlier applications, pre-chilling, tended to produce plants with more shoots. Overall, our experiments indicate that three weeks of chilling at 5°C (752 CU total) is a sufficient chilling regime for forcing 'Sarah Bernhardt' and 'Inspecteur Lavergne' peonies, and 1,000 CU of naturally accumulated chilling is sufficient for nursery production. GA3 applications can reduce the time to emergence and flowering, as well as increase the numbers of total shoots and flowering shoots. Timing of GA3 application is flexible; it can be applied right after rooting, before the chilling period, just before greenhouse forcing, or after shoots have begun to emerge. Plant growth retardant applications had a little effect on the growth of tested cultivars, but all plants treated with growth retardants are generally darker green in color. Additionally, growth retardant applications have some positive effects on flowering. / Doctor of Philosophy / Herbaceous peonies (Paeonia lactiflora Pall.) are common perennials used both in gardens and the landscape as well as for cut flowers. Peonies require a chilling period to break dormancy but not for flower bud differentiation. For all studies, two peony cultivars, Sarah Bernhardt and Inspecteur Lavergne, 3 to 5 eye small crowns from Holland were potted in 3.8-L pots in mid November of 2017 and 2018. Our overall objective was to determine if we could manipulate chilling time, along with application of gibberellic acid (GA3) and growth retardants, to produce marketable containerized peonies from a small crown in a single season (November to May). We evaluated chilling, GA3 and a growth retardant (uniconazole) under controlled chilling and greenhouse forcing conditions. We evaluated the effects of plant growth retardants (uniconazole or paclobutrazol) applied with different methods (fall drenches or spring sprenches) at different stages of production on the growth and development of containerized peony under nursery conditions. To determine the best timing for spring GA3 applications under nursery conditions, we applied three models based on natural chilling accumulation. We also evaluated GA3 effects on peony bud differentiation and development during controlled chilling and early forcing, as well as growth and flowering. Overall, 3 weeks chilling at 5°C [752 chilling units (CU) total] is a sufficient chilling regime for forcing 'Sarah Bernhardt' and 'Inspecteur Lavergne' peonies, and 1000 CU naturally accumulated chilling is sufficient for nursery production. GA3 applications can reduce the time to emergence and flowering, as well as increase the numbers of shoots and flowering shoots. Timing of GA3 application is flexible, it can be applied right after rooting, after the chilling period, or after shoots have begun to emerge. Plant growth retardant applications had little effect on plant growth of either cultivar, but all plants treated with growth retardants were darker green in color. Additionally, growth retardant applications had some positive effects on flowering.

Loblolly pine (Pinus taeda) and coppiced sweetgum (Liquidambar styraciflua) seedlings were grown in competition with a native weed community using soil and seed bank collected near Appomattox, Virginia. Seedlings and weeds were exposed to CO₂ (ambient and elevated) and water (water stressed and well watered) treatments for approximately one growing season in closed top chambers. Weed growth had an effect on tree growth, but the amount of variation in tree biomass explained by weed biomass was very low. It appears that the tree seedlings benefited more from available resources than the herbaceous weeds. The influence of competition with loblolly pine and elevated CO₂ did not have an influence on total weed biomass; however, it did favor C3 weed community development regardless of water availability. This suggests that weed community composition may shift toward C₃ plants in a future elevated CO₂ atmosphere. Loblolly pine height, diameter, needle, shoot and total biomass were significantly greater in the well watered treatment than the water stressed treatment. Pine root, needle, shoot and total biomass were significantly greater in the elevated treatment than the ambient treatment. While not significant, root biomass of water stressed pine seedlings was 63% greater in the elevated CO₂ treatment than the ambient treatment. There was a significant water and CO₂ interaction for pine root:shoot ratio. Under elevated CO₂, root:shoot ratio was significantly greater in the water stressed treatment than the well watered treatment. In contrast, root:shoot ratio in the ambient treatment was nearly identical under both water treatments. These results indicate that loblolly pine will respond favorably in an elevated CO₂ atmosphere, even under dry conditions. The coppiced sweetgum seedlings responded favorably to well watered conditions with significant increases in leaf area, specific leaf area, leaf, shoot and total biomass compared to water stressed conditions. Leaf, root, shoot+stump and total biomass of sweetgum significantly increased and specific leaf area decreased under elevated CO₂ compared to ambient CO₂, but differences were smaller than previous findings. This indicates that coppicing may dampen the growth response to elevated CO₂, at least in the initial growth stage after coppicing. / Master of Science

Successional trends in herbaceous plant production and diversity were studied in an age sequence of sites, i.e. chronosequence, inferred to represent a meadow to aspen to fir to spruce-fir sere. Primary production was observed to decrease in a linear fashion with successional development. Three components of diversity; richness, heterogeneity, and equitability or evenness, each had low early successional values, reaching maximum diversity in mid-succession, and declining to intermediate levels with maturity. The magnitude of these trends varied greatly, depending on the methods used to determine plant dominance. Characteristics of various dominance indices and their applicability to this study were examined. Mean daily photosynthetic biomass was found to be an especially appropriate index of dominance for studying these seasonally dynamic communities. A checklist of 141 vascular plant taxa encountered in the study is included. The flora was determined to be exceedingly species rich, with values of the calculated diversity indices among the highest reported in the literature reviewed. The limitations of diversity indices to sampled data is discussed.