To investigate the localization and targeting of sodium channels in neurons, an efficient means of gene delivery will need to be established. Two amplicon-based viral approaches and a recombinant whole virus approach were attempted in order to package and express RIIA sodium channel tagged with a c-myc epitope (RIIA-myc) with the ultimate purpose of developing a Herpes virus-based model system for targeting studies. / Immunofluorescent staining of transfected epithelial cells was carried out to demonstrate that constructs created for use in these HSV-based approaches were capable of a high level of expression of RIIA-myc. Measurements of $ beta$-galactosidase reporter gene expression observed in cultured cells infected with RIIA amplicon virus suggested successful packaging of amplicon DNA. However, RIIA-myc expression from amplicon virus was not apparent, which may suggest recombination events occurred upon packaging of constructs. Difficulties in selection for recombinants with acyclovir prevented the recombinant whole virus approach from being pursued.

Bibliography: leaves 82-114. / viii, 114, [32] leaves, [14] leaves of plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Depts. of Microbiology and Immunology, 1993

Respiratory disease is an important cause of wastage in the Australian horse racing industry and viruses are frequently suspected as aetiological agents of respiratory disease or poor performance by clinicians and trainers but confirmation is seldom attempted. This thesis deals with the potential role of equine herpes virus types 1, 2, 4 and 5 in upper respiratory disease and poor performance in horses in Western Australia. The methodology selected for the identification of equine herpes viruses in tissues of horses was polymerase chain reaction (PCR) and therefore individual PCR assays were developed for the detection of each herpes virus, and then a nested multiplex PCR was developed to detect all four viruses. There was good correlation between the multiplex PCR for the detection of EHV and the detection of virus by isolation in cell culture, although a combination of the 2 techniques provided greater sensitivity than either technique alone. The multiplex PCR described appeared equally sensitive as specific PCR assays using a single set of primers for each individual virus but reduced labour and reagent costs. As latency is a well recognised phenomenon in the equine herpes viruses and the horse is subjected to a number of stresses which might induce reactivation of latent infections, it was hypothesised that there would be a background level of replication of the equine herpes viruses in clinically normal horses. Nasal swabs and peripheral blood leukocytes (PBL) were obtained from 282 clinical normal horses and examined for EHV. The results clearly demonstrated the widespread occurrence of EHV in the clinically healthy horses. The rate of detection of different types of EHV varied, as did the prevalence in young and adult horses. The most common EHV detected was EHV5: in 83.2% of 131 of horses <2 years of age; in 40% of horses >2 years of age. A prospective clinical study was conducted whereby respiratory tract samples and PBL from adult horses with respiratory disease and/or poor performance were examined for equine herpes viruses; the aim was to determine a possible association between equine herpes virus infection and respiratory disease and/or poor performance. The relative incidence of factors identified in the history, signalment, physical and laboratory evaluation of horses in the study population was compared between horses from which EHV was identified in respiratory samples and horses negative for equine herpes virus. The results indicated that equine herpes viruses were important causes of respiratory disease in the study population, and that haematological and cytological data were a poor indicator of such equine herpes virus infection. The occurrence of equine herpes virus in nasal swabs and PBL of weaned or unweaned foals from Thoroughbred breeding establishments was determined and provided data on the occurrence of EHV in association with respiratory disease. EHV5 was detected in nasal swabs and/or PBL at a high prevalence rate in healthy foals and yearling horses but its occurrence was not associated with clinical signs of respiratory disease. In contrast, EHV2 was detected more commonly in nasal swabs and/or PBL from foals with respiratory disease than in similar samples from healthy horses. Experimental infection of 8 horses with EHV2 was attempted and induced clinical signs of respiratory disease, but less severe than observed in the epidemiological studies. The results suggested that EHV2 is associated with mild upper respiratory tract infection in young horses.

Proefschrift Universiteit van Amsterdam. / Met lit. opg. - Met samenvatting in het Nederlands.

Thesis (Ph.D.) -- McMaster University, 1998. / Includes bibliographical references (leaves 333-365). Also available via World Wide Web.

Diss. (sammanfattning) Göteborg : Univ., 2007. / Härtill 5 uppsatser.

Universiẗat, Diss.--Heidelberg, 2005. / Zusfassung in dt. und engl. Sprache.

Thesis (Ph.D.)--Aberdeen University, 2009. / Includes bibliographical references.

Thesis (Ph. D.)--University of Chicago, Pritzker School of Medicine, Committee on Genetics, March 2003. / Includes bibliographical references. Also available on the Internet.

Proefschrift Rijksuniversiteit Groningen. / Auteur op omslag: Gerard Schellekens. Met lit. opg. - Met samenvatting in het Nederlands en Engels.