The thesis deals with monitoring of the yeast's impact to the chemical and sensorial characteristics of produced wine. The aim of experimental part was obtaining of the aroma-active substances in the fermenting must and wine Hibernal and wine Pinot Blanc. SPME-GC-MS methods were used for identify wines and must aroma. The same autochthonous yeasts were used for wine production. This yeast was isolated from surface of grapes in our laboratory. It was found that both wines have a similar aromatic profile. The next aim was the monitoring of chemical parameters of samples and their changes. For this measurement were used spectrophotometric methods and HPLC methods. The last goal of the thesis was the isolation and identification of yeasts from wine Pinot Blanc by PCR-RFLP. These yeasts were then compared with yeasts isolated from Hibernal must. Identification of yeasts were done by amplification 5,8S-ITS DNA stretches using primers ITS1 and ITS4. Restriction endonuclease HaeIII, HinfI, HhaI and TaqI. were used for restriction analysis then.

This diploma thesis deals with the physical changes of grapevines during their ripening. Three grapevine cultivars were tested - Hibernal, Malverina and Perun. Grapes were harvested at three different times and the basic criteria for defining the level of ripeness were observed namely, in particular, the weight of bunch of grapes, the weight of ten vine berries, grape berry firmness, the percentage of dry matter and the percentage of titratable acids, and the ripeness index. The changes of all criteria in time as well as the difference between particular cultivars were observed. Furthermore, the correlation between criteria was assessed. The grape berries were also assessed from the visual point of view before harvesting. Regarding all the data which was collected, the decrease of titratable acids for each grapevine cultivar was proven during the monitored period. For Hibernal, a linear decrease of 26mg/100g per day was observed. Linear decrease was also observed for grape berry firmness of 18 kPa in case of Hibernal, and 24 kPa in case of Malverina. Increase of the percentage of dry matter was observed for all three cultivars. In addition, a correlation between the weight of a grape berry and any other measured criteria was not found. The ripeness index shows a different beginning of ripening of different cultivars.