The Arctic is undergoing a rapid environmental shift with increasing temperatures and precipitations expected to continue over the next century. Yet, little is known about how microbial communities and their underlying metabolic processes will respond to ongoing climatic changes. To address this question, we focused on Lake Hazen, NU, Canada. As the largest High Arctic lake by volume, it is a unique site to investigate microbial responses to environmental changes. Over the past decade, glacial coverage of the lake has declined. Increasing glacial runoff and sedimentation rates in the lake has resulted in differential influx of nutrients through spatial gradients. I used these spatial gradients to study how environmental changes might affect microbial community structure and functional capacity in Arctic lakes. I performed a metagenomic analysis of microbial communities from hydrological regimes representing high, low, and negligible influence of glacial runoff and compared the observed structure and function to the natural geochemical gradients. Genes and reconstructed genomes found in different abundances across these sites suggest that high-runoff regimes alter geochemical gradients, homogenise the microbial structure, and reduce genetic diversity. This work shows how a genome-centric metagenomics approach can be used to predict future microbial responses to a changing climate.

This thesis investigates the relationships between mercury (Hg) and stable isotope of carbon (δ13C) in marine pelagic zooplankton (Calanus spp., Themisto spp. and Euchaeta spp.) with water mass characteristics in the North Water Polynya (NOW) and in the Mackenzie shelf – Amundsen Gulf area. Two ship based sampling field expeditions were carried out in late summer of 2005 and 2006 in both regions on board the CCGS Amundsen. In the North Water (NOW) polynya, higher levels of water Hg, depleted δ18O, lower salinity and lower nitrate levels were measured at sampling locations near the Prince of Wales glacier (POW) on the eastern coast of Ellesmere Island in the Smith Sound area. These results suggest that the glacier may be a source of Hg to this region which, in turn, is responsible for the correspondingly high concentrations of THg and MMHg measured in Calanus spp. and Euchaeta spp. at the same locations. The Mackenzie shelf – Amundsen Gulf region was characterized by fresher surface water properties (low salinity and depleted δ18O) in the western part and was strongly linked to the influence of the Mackenzie River. Higher THg concentrations in zooplankton were associated with larger fractions of both meteoric water and sea-ice melt. These findings suggest that in the western Arctic, inorganic Hg uptake in zooplankton via-absorption near surface water was highly driven by freshwater inputs into the system. Based on the analysis of three main genus Calanus spp. (mostly adult females Calanus hyperboreus), Euchaeta spp. and Themisto spp. (mostly adult Themisto libellula), THg and MMHg concentrations were the highest in the carnivorous copepod Euchaeta spp. in the North Water polynya followed by the omnivorous hyperiid amphipod Themisto spp. The herbivorous copepod Calanus spp. had both the lowest THg and MMHg concentrations in the Eastern and the Western Arctic. In addition, the Western Arctic is the area in which each zooplankton genus had the most depleted carbon and the most enriched nitrogen. The highest concentrations of THg in Calanus spp., Euchaeta spp. and Themisto spp. were measured in the Western Arctic as well as the highest MMHg in Calanus spp. and Themisto spp. The highest %MMHg was calculated in the Archipelago for Themisto spp., in the Eastern Arctic for Euchaeta spp. and in the Western Arctic for Calanus spp. The relationships observed between THg, MMHg, %MMHg and δ13C in all three major zooplankton taxa and water mass properties were in agreement with what have been previously described in the literature. Our findings suggested that both Hg and δ13C can be used as tracers to help understand zooplankton vertical distribution, feeding ecology and ultimately to predict climate changes impact at lower trophic level in the pelagic food web. The implications for marine mammals foraging in these regions are also discussed. / October 2008

High Arctic meromictic lakes are frequently used to generate detailed records of past environmental variability within their sedimentary profiles. However, without detailed analyses of sedimentation influences over time, their paleoclimate records cannot be accurately interpreted. This thesis presents a comprehensive examination of the sedimentology and paleoenvironmental records of meromictic Lake A, Ellesmere Island (83°00’N, 75°30’W), including detailed analysis of the sedimentary history, development and evaluation of several paleoclimate records. The sedimentary record was primarily composed of clastic material but varying inputs of authigenic components from biological and chemical processes in the water column resulted in a complex history of lake sedimentation. Under chemically stratified conditions during the past millennium, annual laminae (varves) formed and were used to develop a sedimentary chronology. Sedimentary pellets were most likely formed by ice-rafting processes and were deposited during high melt years in this perennially ice-covered lake. The pellet frequency record indicated that the twentieth century contained the most frequent reduced ice cover summers during the past millennium, although the 1500s and 1600s were also inferred warm periods. Comparison with instrumental climate data indicated that varve thickness was primarily related to late summer and autumn snowfall in the previous year, which highlighted the importance of snow availability in spring rather than melt energy in sediment transfer to the lake in this non-glacial catchment. The varve thickness and grain size records suggested increased flow competency and autumn snowfall particularly during the late-1000s to early-1100s and first half of the 1900s. Paleoenvironmental records from Lake A corresponded well with many regional proxy records and provided a long-term framework within which to examine observed environmental change along the northern Ellesmere Island coast during the past century. Finally, these analyses would not have been possible without obtaining high quality sediment cores. This research also investigated the effectiveness of a gel seal method for preserving the fragile sediment-water interface during transport and its results could be useful for other sediment core studies. / Thesis (Ph.D, Geography) -- Queen's University, 2008-05-08 13:34:14.044

In June 2005, fieldwork was conducted during the spring snowmelt period at Cape Bounty, Melville Island, Nunavut to examine the relationships between snow accumulation, runoff, and nutrient fluxes in two High Arctic watersheds. The snowcover was quantified by means of eleven depth and three density measurements at 42 survey transects (100 m) distributed throughout the West and East watersheds. River discharge was monitored at the watershed outlets, where water samples were collected four times daily during the first ten days of melt and twice daily once flow receded. Water samples were also collected from headwater and tributary sites in the two watersheds, and samples were analyzed for DOC, DON and DIN (NH4+ and NO3-). An objective terrain classification weighted equally on slope, aspect and land surface curvature was applied to the two watersheds using an ISODATA unsupervised classification scheme to determine watershed SWE. The terrain model confirmed that topography likely explains greater SWE in the West watershed, and provides a method for reproducible estimates of watershed SWE in future years. However, improved methods for estimating SWE in channels and deep snowbanks are required to ensure accurate assessments of watershed SWE. The seasonal trends in DOC, DON, and DIN concentrations and specific fluxes are reported for both watersheds. The export of DON and DIN was strongly correlated with DOC in the West watershed, indicating that the flushing of terrestrial nutrients from surficial soils by snowmelt runoff governs nutrient export. Despite less watershed SWE (51%), the East watershed exported greater specific fluxes of DOC (33%) and DON (43%) during the melt season. This suggests that the East watershed had greater connectivity with OM sources early in the melt season. Furthermore, low DOC:DON ratios (< 15) in the East River indicate that a larger portion of DOM was likely derived from algal or microbial sources in the East watershed relative to the West watershed. The export of DIN was similar in the two watersheds, and results suggest that DIN export was likely controlled by watershed vegetation coverage and runoff volumes during snowmelt. / Thesis (Master, Geography) -- Queen's University, 2008-07-08 00:31:46.107

This thesis investigates the relationships between mercury (Hg) and stable isotope of carbon (δ13C) in marine pelagic zooplankton (Calanus spp., Themisto spp. and Euchaeta spp.) with water mass characteristics in the North Water Polynya (NOW) and in the Mackenzie shelf – Amundsen Gulf area. Two ship based sampling field expeditions were carried out in late summer of 2005 and 2006 in both regions on board the CCGS Amundsen. In the North Water (NOW) polynya, higher levels of water Hg, depleted δ18O, lower salinity and lower nitrate levels were measured at sampling locations near the Prince of Wales glacier (POW) on the eastern coast of Ellesmere Island in the Smith Sound area. These results suggest that the glacier may be a source of Hg to this region which, in turn, is responsible for the correspondingly high concentrations of THg and MMHg measured in Calanus spp. and Euchaeta spp. at the same locations. The Mackenzie shelf – Amundsen Gulf region was characterized by fresher surface water properties (low salinity and depleted δ18O) in the western part and was strongly linked to the influence of the Mackenzie River. Higher THg concentrations in zooplankton were associated with larger fractions of both meteoric water and sea-ice melt. These findings suggest that in the western Arctic, inorganic Hg uptake in zooplankton via-absorption near surface water was highly driven by freshwater inputs into the system. Based on the analysis of three main genus Calanus spp. (mostly adult females Calanus hyperboreus), Euchaeta spp. and Themisto spp. (mostly adult Themisto libellula), THg and MMHg concentrations were the highest in the carnivorous copepod Euchaeta spp. in the North Water polynya followed by the omnivorous hyperiid amphipod Themisto spp. The herbivorous copepod Calanus spp. had both the lowest THg and MMHg concentrations in the Eastern and the Western Arctic. In addition, the Western Arctic is the area in which each zooplankton genus had the most depleted carbon and the most enriched nitrogen. The highest concentrations of THg in Calanus spp., Euchaeta spp. and Themisto spp. were measured in the Western Arctic as well as the highest MMHg in Calanus spp. and Themisto spp. The highest %MMHg was calculated in the Archipelago for Themisto spp., in the Eastern Arctic for Euchaeta spp. and in the Western Arctic for Calanus spp. The relationships observed between THg, MMHg, %MMHg and δ13C in all three major zooplankton taxa and water mass properties were in agreement with what have been previously described in the literature. Our findings suggested that both Hg and δ13C can be used as tracers to help understand zooplankton vertical distribution, feeding ecology and ultimately to predict climate changes impact at lower trophic level in the pelagic food web. The implications for marine mammals foraging in these regions are also discussed.

This study examined the impact of active layer detachments (ALDs) on seasonal dissolved nitrogen (N) export from continuous permafrost headwater catchments at the Cape Bounty Arctic Watershed Observatory (CBAWO), Melville Island, Nunavut. Runoff samples collected throughout the summer of 2012 from an undisturbed catchment (Goose; GS) and from one that was disturbed by ALDs (Ptarmigan; PT) were analyzed for dissolved inorganic ions and species of total dissolved N (TDN), including dissolved organic N (DON) and dissolved inorganic N (DIN; ammonium (NH4+), nitrite (NO2-), and nitrate (NO3-)). Rainfall samples were also collected for dissolved ions analyses. Select runoff and rainfall samples were analyzed for stable isotopes of nitrogen and oxygen in NO3- (δ15N-NO3- and δ18O-NO3-, respectively) to determine its origin streamwater. Data from 2012 were compared to predisturbance data to assess the long-term effects of ALDs on N export. ALDs increased the proportion of DIN/TDN in PT from 4% (predisturbance) to 37% in 2012. The increase in DIN/TDN in PT largely resulted from significantly higher NO3- in runoff. Values of δ18O and δ15N-NO3- as well as correlations between NO3- and major ions indicated that the higher NO3- in PT was due to the exposure of mineral soils in ALDs, which likely reduced NO3- sinks (e.g. plant uptake) and increased inputs of nitrified-NO3-. Values of δ18O-NO3- during initial runoff showed that NO3- supplied from the snowpack overwhelmed NO3- sinks in PT, leading to a twelve-fold higher peak NO3- concentration relative to GS. Low δ18O-NO3- values in runoff during stormflow indicated that inputs of DIN from rainfall (1545 ± 148 and 1838 ± 174 g N-DIN to GS and PT, respectively) supplied less than 30% of the NO3- in both streams, and that exceptionally high NO3- concentrations in PT resulted from flushing of mineralized-NO3- from the mineral soils. Seasonal DIN flux was 95% higher in PT relative to GS, because NO3- export from PT was 27 times that of GS. This is the first study to show that ALDs can have persistent impacts on DIN export from High Arctic watersheds, and that this results from enhanced export of mineralized-NO3-. / Thesis (Master, Geography) -- Queen's University, 2014-05-27 10:30:38.874

This thesis investigates the relationships between mercury (Hg) and stable isotope of carbon (δ13C) in marine pelagic zooplankton (Calanus spp., Themisto spp. and Euchaeta spp.) with water mass characteristics in the North Water Polynya (NOW) and in the Mackenzie shelf – Amundsen Gulf area. Two ship based sampling field expeditions were carried out in late summer of 2005 and 2006 in both regions on board the CCGS Amundsen. In the North Water (NOW) polynya, higher levels of water Hg, depleted δ18O, lower salinity and lower nitrate levels were measured at sampling locations near the Prince of Wales glacier (POW) on the eastern coast of Ellesmere Island in the Smith Sound area. These results suggest that the glacier may be a source of Hg to this region which, in turn, is responsible for the correspondingly high concentrations of THg and MMHg measured in Calanus spp. and Euchaeta spp. at the same locations. The Mackenzie shelf – Amundsen Gulf region was characterized by fresher surface water properties (low salinity and depleted δ18O) in the western part and was strongly linked to the influence of the Mackenzie River. Higher THg concentrations in zooplankton were associated with larger fractions of both meteoric water and sea-ice melt. These findings suggest that in the western Arctic, inorganic Hg uptake in zooplankton via-absorption near surface water was highly driven by freshwater inputs into the system. Based on the analysis of three main genus Calanus spp. (mostly adult females Calanus hyperboreus), Euchaeta spp. and Themisto spp. (mostly adult Themisto libellula), THg and MMHg concentrations were the highest in the carnivorous copepod Euchaeta spp. in the North Water polynya followed by the omnivorous hyperiid amphipod Themisto spp. The herbivorous copepod Calanus spp. had both the lowest THg and MMHg concentrations in the Eastern and the Western Arctic. In addition, the Western Arctic is the area in which each zooplankton genus had the most depleted carbon and the most enriched nitrogen. The highest concentrations of THg in Calanus spp., Euchaeta spp. and Themisto spp. were measured in the Western Arctic as well as the highest MMHg in Calanus spp. and Themisto spp. The highest %MMHg was calculated in the Archipelago for Themisto spp., in the Eastern Arctic for Euchaeta spp. and in the Western Arctic for Calanus spp. The relationships observed between THg, MMHg, %MMHg and δ13C in all three major zooplankton taxa and water mass properties were in agreement with what have been previously described in the literature. Our findings suggested that both Hg and δ13C can be used as tracers to help understand zooplankton vertical distribution, feeding ecology and ultimately to predict climate changes impact at lower trophic level in the pelagic food web. The implications for marine mammals foraging in these regions are also discussed.

<p> This research indicates that present methods of water supply and sewerage for high arctic communities are inadequate from the point of view of health, aesthetics and economics. This thesis examines these present methods and their problems.</p> <p> Field work was conducted in three communities of the Eastern Canadian Arctic. Data were collected with regard to, - the biological quality of the drinking water and raw water sources, the quantities of river water available, the soil conditions, the construction equipment and generating capacities of the small communities, water consumption, and the sizes and types of storage tanks within the communities.</p> <p> In order to improve existing conditions, an entirely new method of water supply is developed in which water is intermittently distributed through electrically traced pipes to storage tanks within all the buildings. Computer programs are presented which will optimize the design on the basis of net annual cost. The related problems of water quality, power supply and sewerage are also examined and social, health and aesthetic effects are considered.</p> <p> The results are novel in many respects: Distributed storage allows the use of very small diameter pipes and results in very low capital, construction and operating costs.</p> / Thesis / Master of Engineering (MEngr)

Plankton are generally considered good indicators for ocean climate variability, but plankton data from the Arctic are still comparatively scarce. Due to this scarcity of information, the prevalence of vertical migration behaviour at high latitude is still debated. Atlantic inflow is a key process governing biological diversity in the Arctic Ocean, and the location of the Svalbard archipelago makes it an ideal study area to monitor this inflow. Comparing the zooplankton community within the fjords of Svalbard at various latitudes allowed us to assess the influence of Atlantic inflow and any subsequent changes in zooplankton composition that may have implications for higher trophic levels. Using sediment traps deployed on oceanic moorings, Chapter 3 of this thesis analysed long term observations from sea-ice dominated Rijpfjorden for the first time, and compared the zooplankton to Atlantic Water influenced Kongsfjorden. Chapters 4 and 5 investigated the spatial relevance of our moored observations using shipboard observations, and chapters 6 and 7 present observations of vertical migration across a range of conditions. Kongsfjorden was dominated by Calanus copepods associated with Arctic and Atlantic water, and strongly influenced by Atlantic Water advection. Rijpfjorden was largely influenced by sea-ice formation with higher proportional abundances of macrozooplankton species. Advection brought Atlantic associated species into Rijpfjorden during warmer years. Prevailing hydrology and bathymetry were highlighted as factors forcing zooplankton distribution, while advection was identified as responsible for much of the observed small scale spatial variation amongst weaker swimmers. At an aggregation scale of 0.5 nautical miles, zooplankton distribution was highly patchy and moored observations could only be reliably expanded outwards to a maximum of 1 nautical mile. Low amplitude diel vertical migration (especially by younger copepodids) was identified in surface waters when a food source was available. These observations must be considered within the dynamic framework of advection highlighted by this thesis.

Until recently it has been widely believed that significant anthropogenic influences on the environment began in Canada following the onset of European colonization. However, our paleolimnological data indicate that centuries prior to European settlement, ponds on Ellesmere and Bathurst Island were impacted by Thule Inuit whalers, whose activities altered nutrient levels in nearby ponds. Two Thule Inuit whaling sites were selected based on input from several archaeologists, to ensure good coverage of the Thule geographic range and proximity to freshwater ponds. Multiple independent paleolimnological proxies have been used to analyze a pond from Ellesmere Island, showing taxonomic shifts in diatoms assemblages coinciding with 1.5 - 2‰ shifts in d15N, during the period of Thule occupation (ca. 1000 – 1670 AD). Increases in the relative abundance of Amphora ovalis, indicate nutrient concentrations above average for the High Arctic. Elevated levels of nitrogen and phosphorus were observed in the pond indicating the continuing influence of nutrient inputs centuries after the abandonment of the camp. Meanwhile, on Bathurst Island, the orientation of the Deblicquy site, such that the large majority of the Thule nutrient inputs are focused towards one of our two study ponds, provided us with the opportunity to compare two ponds that are essentially identical with the exception of the degree of Thule influence. In our “impacted” site, a marked increase in Stephanodiscus minutulus, coincides with a 2‰ shift in d15N. While our a priori determined control site shows no major changes in geochemistry or algal composition. Previous research on Bathurst Island used water chemistry and surface sediment diatoms to construct a diatom-inferred total nitrogen model for Bathurst Island. However, this study was limited by excluding unbuffered, low pH sites which characterize the western half of Bathurst Island. By expanding the previous Bathurst Island dataset to include western sites, we have been able to construct a diatom-inferred pH model which will prove invaluable in future climate research in this region. Together, these three studies serve to highlight the sensitivity of freshwater ecosystems to relatively minor anthropogenic disturbances and represent some of the earliest known anthropogenic impacts on North American aquatic ecosystems. / Thesis (Master, Biology) -- Queen's University, 2007-12-20 15:20:46.342