Os índices de seleção são combinações lineares de valores fenotípicos, resultando numa medida que concentra em único valor, os atributos positivos e negativos de cada genótipo para vários caracteres. Por necessitarem das estimativas das variâncias e covariâncias genotípicas e fenotípicas dos caracteres, esses índices são mais indicados para programas de seleção recorrente. Contudo, existem também outros tipos de índices não lineares, conhecidos como não paramétricos que, por não precisarem das estimativas dos parâmetros genéticos, podem ser utilizados na seleção de cultivares em fase final dos programas de melhoramento. Para essa situação, uma alternativa bastante promissora é o emprego do índice de seleção de cultivares proposto por Garcia (1998) que é uma medida de distância genética entre cada genótipo a um ideótipo. Esta técnica permite a aplicação de teste de comparação de médias e a identificação de genótipos com desempenhos abaixo dos critérios mínimos definidos pelo melhorista. Este trabalho teve como objetivos avaliar a viabilidade do índice proposto por Garcia (1998) na identificação de genótipos superiores de algodão, estimar os parâmetros de estabilidade do índice e verificar quanto da variação fenotípica do índice é de natureza genotípica. Foram utilizados quinze genótipos de algodoeiro do Ensaio Estadual de Algodoeiro Herbáceo (2000/01) avaliados em 27 locais sob o delineamento de blocos ao caso com quatro repetições. Os caracteres avaliados foram produtividade de algodão em caroço (kg/ha), altura de planta (cm), peso de um capulho (g), porcentagem de fibra (%), resistência da fibra (gf/tex), comprimento da fibra (mm), finura da fibra (IM), ramulose (notas) e bacteriose (notas) Os resultados obtidos evidenciaram que o índice proposto por Garcia (1998) identificou com eficiência as cultivares superiores com destaque para BRS AROEIRA, CNPA 97- 4565 e DELTA OPAL. As cultivares mais estáveis foram: CNPA CO 99-01, BRS IPÊ e BRS JATOBÁ, para o índice de seleção e BRS AROEIRA, BRS IPÊ e CNPA 99-01, para a produtividade. Os índices de seleção apresentaram médio e alto coeficiente de determinação genotípica, indicando que é possível antever o sucesso com a seleção utilizando as estimativas do índice proposto por Garcia (1998). Neste aspecto, recomenda-se complementar o emprego do índice de seleção com informações sobre a estabilidade e adaptabilidade das cultivares selecionadas sob condições favoráveis e desfavoráveis. / Selection indices are linear combinations of phenotypic values, which combine in one unique value the positive and negative attributes of each genotype for the characters evaluated. Because these indices need estimates of genetic and phenotypic variances and covariances, their use is best suited in recurrent selection programs. However, other types of non-linear indices also exist, and are known as non-parametric indices; these can be used in selection of cultivars at the final stage of breeding programs, since genetic parameter estimates are not a requirement. In this situation, the selection index developed by Garcia (1998) is a promising alternative. This index is an estimate of the genetic distance from each genotype to an "ideotype". This technique allows the comparison of means and the identification of genotypes with performances that meet the minimum requirements chosen by the breeder. The present work had the following objectives: to evaluate the viability of the Garcia index for the identification of superior genotypes, to estimate the stability parameters of the index and verify how much of the phenotypic variation is genotypic. Fifteen genotypes of the Upland cotton Variety Trials (Ensaio Estadual de Algodoeiro Herbáceo) - 2000/01 were evaluated in 27 locations in randomized complete block design with four replications. Characters assessed were: seed cotton yield (kg/ha), plant height (cm), boll mass (g), lint percentage (%), fiber strength (gf/tex), fiber length (mm), lint micronaire (IM), ramulosis (scores) and bacterial blight (scores). The data showed that the Garcia index identified superior cultivars successfully, as BRS AROEIRA, CNPA 97-4565 and DELTA OPAL. The most stable cultivars were: CNPA CO 99-01, BRS IPÊ and BRS JATOBÁ for selection index and BRS AROEIRA, BRS IPÊ e CNPA 99-01 for yield. The selection indices showed medium and high coefficient of genotypic determination indicating that is possible to expect selection success when the Garcia index is used. We suggest to complementing the use of the selection index with informations about stability and adaptability of the selected cultivars at favorable and unfavorable environments.

Submitted by Maria Beatriz Vieira (mbeatriz.vieira@gmail.com) on 2017-03-30T15:37:38Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) dissertacao_leonir_flach.pdf: 318187 bytes, checksum: 18084d61e9bb08da2b5e1c3dcda58b51 (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-04-05T19:09:32Z (GMT) No. of bitstreams: 2 dissertacao_leonir_flach.pdf: 318187 bytes, checksum: 18084d61e9bb08da2b5e1c3dcda58b51 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-04-05T19:10:29Z (GMT) No. of bitstreams: 2 dissertacao_leonir_flach.pdf: 318187 bytes, checksum: 18084d61e9bb08da2b5e1c3dcda58b51 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-04-05T19:10:42Z (GMT). No. of bitstreams: 2 dissertacao_leonir_flach.pdf: 318187 bytes, checksum: 18084d61e9bb08da2b5e1c3dcda58b51 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-06-01 / Sem bolsa / Considerando a importância do tratamento e da manutenção da qualidade da semente durante o armazenamento, objetivou-se através deste trabalho avaliar o desempenho fisiológico de sementes de algodão tratadas quimicamente e armazenadas em condição ambiente. O delineamento experimental foi inteiramente casualizado, com 6 repetições, em esquema fatorial 9 x 6 (tratamentos x época de avaliação). Foram realizados os seguintes tratamentos de sementes: T1 – Testemunha (sem tratamento); T2 – Fungicida Carbendazin 150g/l e Tiran 350g/l (600 ml/100 kg de sementes); T3 – Fungicida Triadmenol 150g/l (200 ml/100 kg de sementes); T4 – Fungicida Pencicurom 250g/l (300 ml/100 kg de sementes); T5 – Fungicida Azoxistrobina 75g/l, Metalaxil-M 37,5g/l e Fludioxonil 12,5 g/l (300 ml/100kg de sementes); T6 – Inseticida Imidacloprido 150 g/l e Tiodicarbe 450g/l (2400 ml/100kg de sementes); T7 – Inseticida Tiametoxam 350g/L (600ml/100kg de sementes); T8 – Composto pelos tratamentos 2, 3, 4 e 6 mencionados acima; T9 – Composto pelos tratamentos 5 e 7. A avaliação da qualidade fisiológica das sementes de algodão foi realizada aos 0, 30, 60, 90, 120 e 150 dias de armazenamento através dos testes de germinação e vigor (baixa temperatura e envelhecimento acelerado). Os resultados permitem constatar que os produtos (triadmenol) e o (carbendazin e tiran + triadmenol + Pencicurom + imidacloprido e tiodicarbe) reduzem os percentuais de germinação e vigor da sementes de algodão. A qualidade fisiológica é negativamente afetada conforme se aumenta o período de armazenamento. / Considering the importance of treatment and maintenance of seed quality during storage, the aim through this work was to evaluate the physiological performance of cotton seeds chemically treated and stored at room temperature. The experimental design was completely randomized with six replications in factorial scheme 9 x 6 (treatments x evaluation time). The following seed treatments were used: T1 - control (no treatment); T2 - Fungicide Carbendazin 150g / l Tiran 350g / l (600 ml / 100 kg seed); T3 - Fungicide Triadmenol 150g / l (200 ml / 100 kg seed); T4 - Fungicide Pencicurom 250g / l (300 ml / 100 kg seed); T5 - Fungicide Azoxystrobin 75g / l, Metalaxyl-M 37,5g / l Fludioxonil 12.5 g / l (300 ml / 100kg seed); T6 - insecticide Imidacloprid 150 g / l Thiodicarb 450g / l (2400 ml / 100kg seed); T7 - insecticide thiamethoxam 350 g / L (600ml / 100kg of seeds); T8 - for treatments compound 2, 3, 4 and 6 mentioned above; T9 - Composed by treatments 5 and 7. The evaluation of the physiological quality of cotton seeds was performed at 0, 30, 60, 90, 120 and 150 days of storage through the germination and vigor tests (low temperature and accelerated aging). The results help determine that the product (triadmenol) and (carbendazin and tyranny + triadmenol + Pencicurom + imidacloprid and thiodicarb) reduce the percentage of germination and vigor of cotton seeds. The physiological quality is adversely affected as increases the storage period.

Submitted by Maria Beatriz Vieira (mbeatriz.vieira@gmail.com) on 2017-04-20T16:05:40Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) dissertacao_idimar_leoni.pdf: 641218 bytes, checksum: 4818db3c1e7883b6f212dcb23e444e9e (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-04-24T12:29:10Z (GMT) No. of bitstreams: 2 dissertacao_idimar_leoni.pdf: 641218 bytes, checksum: 4818db3c1e7883b6f212dcb23e444e9e (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Aline Batista (alinehb.ufpel@gmail.com) on 2017-04-24T12:29:45Z (GMT) No. of bitstreams: 2 dissertacao_idimar_leoni.pdf: 641218 bytes, checksum: 4818db3c1e7883b6f212dcb23e444e9e (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-04-24T12:30:54Z (GMT). No. of bitstreams: 2 dissertacao_idimar_leoni.pdf: 641218 bytes, checksum: 4818db3c1e7883b6f212dcb23e444e9e (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-10-02 / Sem bolsa / O presente trabalho é o resultado da realidade dos fatos que aconteceram à cultura do algodoeiro no Estado do Mato Grosso. O trabalho foi realizado com o objetivo de apresentar a área cultivada com o algodoeiro nas safras de 2009/10 à 2014/15. Para o enriquecimento deste trabalho, foram avaliados o Market Share de cada empresa obtentora e comerciante de sementes de algodão e dentro de cada uma, suas cultivares, e aquelas cultivares que foram substituídas e, ou, derivadas através da introgressão ou mesmo outros métodos de transformação para OGM (s). Também foi necessário levantar a forma de cultivo para calcular a demanda de sementes. Paralelamente, através da Taxa de Utilização de Sementes foi estimado a quantidade de sementes salvas por diferença matemática. Para agilidade do levantamento e compilação dos dados, o Estado foi dividido em sete Regionais: 1 Centro (sediado em Campo Verde), 2 Centro Leste (sede em Primavera do Leste), 3 Centro Norte (região de Lucas do Rio Verde), 4 Norte (Sorriso), 5 Médio Norte (Campo Novo do Parecis), 6 Noroeste (Sapezal) e 7 Sul (Rondonópolis). A veracidade do estudo é fruto de um trabalho executado a cada safra em cada talhão para cada cultivar, espaçamento e época de semeadura onde se cultivou algodão no estado do Mato Grosso. Através dos dados apresentados nas tabelas e gráficos pode-se conferir a evolução da cultura do algodoeiro, bem como a entrada de novas cultivares de algodão, cultivares com tecnologia OGM e outras também às derivadas das cultivares convencionais. Com a entrada das cultivares de algodão OGM e nível de tecnologia mais afinado, cada produtor foi se adequando a um sistema de cultivo planejado e programado para as culturas subsequentes. O obtentor do setor privado dominou o mercado das cultivares de algodão nas últimas safras. Dois obtentores detém mais de 80% da fatia de mercado de sementes de algodão no estado de Mato Grosso. Há baixíssima relação entre o número de cultivares adotadas e número de cultivares lançadas, pelos agricultores. / The current abstract is the result of the reality of facts that happened to the cotton culture in the State of Mato Grosso. The abstract was written with the purpose to present the cotton culture area from the 2009/10 to 2014/15 harvest seasons. To better present this abstract, the Market Share of each major and retail cotton seed company was evaluated and inside each one of their cultivars, and those cultivars that were substituted and, or, derivated through introgression or even through other transformation methods to GMO (s). It was also necessary to know the planting method to calculate the demand of seeds, parallel to that, through the seed utilization rate, it was estimated the amount of seeds saved according to mathematic difference. To speed the collection and compilation of data, the State was divided in seven regions: 1 Center (based in Campo Verde), 2 Eastern Central (based in Primavera do Leste), 3 Northern Central (based in Lucas do Rio Verde), 4 North (Sorriso), 5 Mid-Northern (Campo Novo do Parecis), 6 Northwest (Sapezal) and 7 South (Rondonópolis). The veracity of this study is due to a work done on each harvest season, in every section for each cultivar, planting distance and time in which cotton was cultivated in the State of Mato Grosso. Through the presented data on the tables and graphics we can see the evolution of the cotton culture, as well as the new cotton cultivars, cultivars with the GMO technology and also the ones derivated from the conventional cultivars. Along with the release of the GMO cotton cultivars and a more refined level of technology, each farmer complied to a planned and programed planting system to the subsequent cultures. The breeders of the private sector dominated the cotton cultivars market share for the last harvest seasons. Two breeders have the right of more than 80% of the cotton seeds market share in the state of Mato Grosso. There is a very small relation between the number of chosen cultivars and the number of released cultivars by the farmers.

Orientador: Enes Furlani Júnior / Banca: Marcelo Andreotti / Banca: Salatiér Buzetti / Banca: Luiz Henrique Carvalho / Banca: Edivaldo Cia / Resumo: A aplicação de nitrogênio em cobertura e a manutenção da palhada, realizadas na semeadura direta, devem respectivamente, atender as necessidades da cultura e promover a conservação do solo. Dessa forma o presente trabalho teve por objetivo avaliar o efeito da aplicação de nitrogênio em pré-semeadura do algodoeiro e das plantas de cobertura, implantadas em semeadura direta, sobre as propriedades físicas e químicas do solo e sobre o desenvolvimento e produtividade do algodoeiro. O delineamento experimental empregado foi o de blocos ao acaso composto por três plantas de cobertura (nabo forrageiro, aveia preta e aveia branca) e quatro doses de nitrogênio (0, 30, 60, e 90 kg de N ha-1) aplicadas sobre a palhada do milheto e em pré-semeadura do algodoeiro. Aos 80 dias após a emergência do algodoeiro, nos anos agrícolas de 2006/07 e 2007/08 foram coletas folhas para a análise foliar dos macronutrientes. No mês de abril de 2006, abril de 2007 e abril de 2008 foram realizadas as colheitas das parcelas experimentais com a cultura do algodoeiro. Em maio de 2006 e maio de 2007 foram retiradas amostras de solo nas profundidades de 0-5, 5-10 e 10-20 cm com o intuito de analisar os nutrientes do solo e em agosto de 2006 e agosto de 2007 foram realizadas as análises físicas do solo para as profundidades de 0-15, 15-30, 30-45 e 45-60 cm. De acordo com os resultados obtidos verifica-se em função da utilização de sulfato de amônio em doses crescentes ocorreu diminuição do pH, Ca e do Mg em superfície do solo, assim como o aumento do Al+3 trocável e do teor de enxofre até 20 cm. A utilização do nabo forrageiro proporcionou aumento dos teores de K nas folhas do algodoeiro e a utilização de doses de N até 90 kg ha-1 em pré-semeadura proporcionaram aumento nos teores de N e S. O nabo forrageiro é uma planta de cobertura que proporciona aumento da produtividade do algodoeiro / Abstract: The application of nitrogen and former crop residues maintenance, held in the till, must respectively meet the needs of culture and promote soil conservation.This study aimed to evaluate the effect of nitrogen pre-sowing of cotton crop and cover crop, established in tillage on the physical and chemical properties of soil and cotton plant development and yield. The experimental design was a randomized block consisting of three cover crops (radish, white oat and black oat) and four nitrogen levels (0, 30, 60, and 90 kg N ha-1) applied to the millet and cotton pre-sowing. At 80 days after cotton emergence in season of 2006/07 and 2007/08 leaves were collected for macronutrients foliar analysis. In April 2006, April 2007 and April 2008 cotton crop plots were harvested. In May 2006 and May 2007 soil samples were taken from depths of 0-5, 5-10 and 10-20 cm in order to analyze the soil nutrients and, in August 2006 and August 2007 were carried out the physical soil analysis to depths of 0-15, 15-30, 30-45 and 45-60 cm. The results obtained in depending on the use of ammonium sulphate in increasing doses, there was a decrease in pH, Ca and Mg in the surface soil, as well as the increase in exchangeable Al+3 and the sulfur content of up to 20 cm. The radish provides increased levels of K in leaves of cotton, and the use of doses N 90 kg ha-1 in presowing provided increased levels of N and S. The radish is a cover crop that provides yield increased. The cotton yield increased with the use of N 90 kg ha-1 in pre-sowing / Doutor

Epilobium hirsutum L. (Onagraceae) is a flowering, tall and perennial plant and native to Eurasia. It shows analgesic, anti-microbial and anti-proliferative activity, and it is used in our country as an alternative medicine. The pharmacological effect of Epilobium hirsutum L. could be explained by the presence of polyphenolics including steroids, tannins and flavonoids in the aerial parts. Viscum album L. (Loranthaceae) is a shrub that grows as an epiphyte on the branches of deciduous trees. It involves in the enhancement of macrophage phagocytic and cytotoxic mediated abilities as well as the strengthening the immune system. CYP24A1 and CYP27B1 are members of cytochrome P450 superfamily and the most important enzymes involved in the metabolism of vitamin D3. CYP27B1 and CYP24A1 are mitochondrial enzymes and also known as 25-hydroxyvitamin D3 1alpha-hydroxylase and 24-hydroxylase, respectively. CYP24A1 involves in 24-hydroxylation of 25-OH-D3 and 1,25-(OH)2D3 which is required for the catabolism of vitamin D3 compounds while CYP27B1 involves in 1&alpha / -hydroxylation of 25-OH-D3 into 1,25-(OH)2D3. In this study, in vivo effects of Epilobium hirsutum and Viscum album (subspecies growing on pine-trees-subsp. austriacum (Wiesb.) Vollmann) on rat liver CYP24A1 and CYP27B1 mRNA and protein expressions were investigated. To achieve this goal, 37.5 mg water extract of Epilobium hirsutum L./kg body weight/day was intraperitoneally injected to male rats for 9 days. To study the effect of Viscum album L., 10 mg water extract of Viscum album L./kg body weight/day was injected with the same conditions. After decapitation, livers were removed and S1.5 fractions were prepared. Effects of Epilobium hirsutum L. and Viscum album L. on rat liver mRNA and protein expressions were analyzed by qRT-PCR and western blotting, respectively. Epilobium hirsutum L. extract caused 31% and 18% decrease in rat liver CYP24A1 (p&lt / 0.0001) and CYP27B1 (p&lt / 0.05) protein expressions, respectively. The effect of Epilobium hirsutum L. on mRNA expression of CYP24A1 could not be observed, because CYP24A1 mRNA was almost undetectable in liver. Injection of Epilobium hirsutum L. to rats caused 2.7 fold increase in mRNA expression of CYP27B1 with respect to controls and normalized with GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) expression as an internal reference (p&lt / 0.005). Viscum album L. caused 17% decrease in CYP24A1 protein expression (p&lt / 0.05). When rats injected with plant extract of Viscum album L., 18% decrease in CYP27B1 protein expression was observed (p&lt / 0.05). The effect of Viscum album L. on mRNA expression of CYP24A1 could not be observed since CYP24A1 mRNA was almost undetectable in liver. Injection of Viscum album L. to rats caused 3.8 fold increase in mRNA expression of CYP27B1 with respect to controls and normalized with GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) expression as an internal reference (p&lt / 0.005). In conclusion, vitamin D3 metabolism may be affected by medicinal plants Epilobium hirsutum L. and Viscum album L. due to the changes in mRNA and protein expressions of CYP24A1 and CYP27B1 enzymes.

Epilobium hirsutum L. (Onagraceae), a medicinal plant known as hairy willow herb, has been used by people all around the world for treatment or prevention of inflammation, adenoma, rectal bleeding, menstrual disorders, constipates, and prostate. It contains polyphenolics including steroids, tannins such as gallic, ellagic, and p-coumaric acids and flavonoids such as myricetin, isomyricetin, and quercetin. Polyphenols have been known for their multiple biological health benefits, including antioxidant activities. Viscum album L. (Loranthaceae), a species of mistletoe, contains lectins, polypeptides, mucilage, sugar alcohols, flavonoids, lignans, triterpenes, and phenylallyl alcohols. The leaves and twigs of Viscum album L., taken as tea, have been traditionally used for hypertension, stomachache, diarrhea, diabetes, dysuria and also as analgesic and cardiotonic agent in Anatolia, Turkey. In addition, in Europe, sterile extracts of Viscum album L. are among the most common herbal extracts applied in cancer treatment and have been used as prescription drugs, while in US, considered as dietary supplement. Flavin-containing monooxygenases are FAD-containing phase I enzymes responsible for the oxidation of wide-range of nucleophilic nitrogen, sulfur, phosphorus, and selenium heteroatom-containing drugs such as tamoxifen, v methimazole and imipramine, pesticides, neurotoxins, and other chemicals using NADPH as cofactor. The aim of this study was to determine the in vivo effects of Epilobium hirsutum L. and Viscum album L. (subspecies growing on pine trees-subsp. austriacum (Wiesb.) Vollmann) on FMO activity, mRNA and protein expressions in rat liver. The water extracts of Epilobium hirsutum L. (37.5 mg/kg body weight) and Viscum album L. (10 mg/kg body weight) were injected intraperitonally (i.p) into Wistar albino rats for 9 consecutive days. Following the decapitation, the livers were removed and microsomal fractions were prepared by differential centrifugation. Rat liver microsomal FMO activity using methimazole as substrate, mRNA expression by quantitative Real-Time PCR, and protein expression by Western Blot were determined. The results showed that water extract of Epilobium hirsutum L. has no significant effect on FMO activity / however, it decreased significantly (p&lt / 0.05) FMO3 protein and mRNA expression 27.71% and 1.41 fold, respectively, compared as controls. Water extract of Viscum album L. decreased mRNA (2.56 fold), and protein expressions (27.66%) as well as enzyme activity (19%) of FMO with respect to controls. In conclusion, our current data suggest that the metabolism of xenobiotics including drug molecules by FMO-catalyzed reactions may be altered due to the changes in FMO expression and activity by medicinal plants Epilobium hirsutum L. and Viscum album L.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-12-12T18:27:29Z No. of bitstreams: 1 SauloHenriqueGomesDeAzevedo_TESE.pdf: 2820544 bytes, checksum: f1294c696450b1d2d679c0c9eac313f7 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-12-13T20:40:34Z (GMT) No. of bitstreams: 1 SauloHenriqueGomesDeAzevedo_TESE.pdf: 2820544 bytes, checksum: f1294c696450b1d2d679c0c9eac313f7 (MD5) / Made available in DSpace on 2017-12-13T20:40:34Z (GMT). No. of bitstreams: 1 SauloHenriqueGomesDeAzevedo_TESE.pdf: 2820544 bytes, checksum: f1294c696450b1d2d679c0c9eac313f7 (MD5) Previous issue date: 2017-09-01 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / No contexto energ?tico mundial, o biodiesel tem despontado como uma importante fonte de energia renov?vel, sobretudo devido ? facilidade de produ??o do mesmo e ? variedade de mat?ria-prima dispon?vel mundialmente. Especificamente no Brasil, a ind?stria de biodiesel tem apresentado importante crescimento de produ??o, tendo em vista as leis federais que obrigam a mistura do B100 (biodiesel) ao diesel f?ssil. Diante da import?ncia desta ind?stria para o Brasil, este trabalho buscou avaliar o potencial do ?leo de sementes de algod?o para a produ??o de biodiesel a partir de tr?s t?cnicas: transesterifica??o, utilizando etanol, interesterifica??o, usando acetato de metila, e interesterifica??o, com acetato de metila e adi??o de solu??o aquosa de ?cido ac?tico. As rea??es ocorreram em condi??es supercr?ticas, em reator tubular de fluxo cont?nuo com volume de 15 mL. O rendimento m?ximo obtido pela transesterifica??o foi de 75,05%, na press?o de 150 e 200 bar, 375 ?C, raz?o molar ?leo/etanol 1:40, e tempos espaciais 40 min (150 bar) e 30 min (200 bar). J? na rota utilizando acetato de metila, o maior rendimento foi de 99,51% (?steres met?licos de ?cidos graxos + triacetina), obtido em 35 min, raz?o m?ssica ?leo/acetato de metila 1:3, press?o 200 bar e 350 ?C, utilizando 20%, em massa, de solu??o aquosa de ?cido ac?tico com concentra??o de 26%. Foram observados elevados valores de decomposi??o do biodiesel, em ambas as rotas, devido ?s altas concentra??es de triglicer?deos poliinsaturados no ?leo e ?s severas condi??es do processo. / In the global energy context, biodiesel has emerged as an important source of renewable energy, mainly due to its ease of production and the variety of raw material available worldwide. Specifically in Brazil, the biodiesel industry has shown significant production growth, due to the federal laws that require the mixing of B100 (biodiesel) with fossil diesel. Considering the importance of this industry to Brazil, this work sought to evaluate the potential of cotton seed oil for the production of biodiesel from three techniques: transesterification using ethanol, interesterification using methyl acetate and interesterification with methyl acetate and addition of aqueous acetic acid solution. The reactions occurred under supercritical conditions in a continuous flow tubular reactor with a volume of 15 mL. The maximum yield obtained by transesterification was 75.05%, at 150 and 200 bar pressure, 375 ?C, 1:40 oil/ethanol molar ratio, and spatial times 40 min (150 bar) and 30 min (200 bar). In the other method, using methyl acetate, the highest yield was 99.51% (methyl esters of fatty acids+ triacetin) obtained in 35 min, oil/methyl acetate ratio 1:3, pressure 200 bar and 350 ?C, using 20%, by mass, of aqueous acetic acid solution with a concentration of 26%. High values of biodiesel decomposition were observed in both routes due to the high concentrations of polyunsaturated triglycerides in the oil and the severe process conditions.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-19T13:34:44Z No. of bitstreams: 5 ale.pdf: 2255523 bytes, checksum: 7080bc1f5ac0e98cef0030c1f04a51b3 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T10:24:21Z (GMT) No. of bitstreams: 5 ale.pdf: 2255523 bytes, checksum: 7080bc1f5ac0e98cef0030c1f04a51b3 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Made available in DSpace on 2015-02-20T10:24:21Z (GMT). No. of bitstreams: 5 ale.pdf: 2255523 bytes, checksum: 7080bc1f5ac0e98cef0030c1f04a51b3 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) Previous issue date: 2013-02-21 / Na atualidade, n?o se pode desconsiderar a necessidade de desenvolvimento de tecnologias que suportem as demandas por energias renov?veis. Uma das vias para a produ??o de energia renov?vel ? a transforma??o de biomassas vegetais em combust?veis l?quidos. Os res?duos agroindustriais s?o candidatos potenciais como insumos para essa nova ind?stria de energia. Dessa forma a presente disserta??o teve por objetivo avaliar o potencial da torta de caro?o de algod?o e do baga?o de malte com farinha de pupunha como mat?rias-primas para a produ??o de etanol de segunda gera??o e otimizar etapa de pr?-tratamento ?cido do material lignocelul?sico necess?ria para a utiliza??o da celulose. Inicialmente foi determinada a composi??o centesimal da torta de caro?o de algod?o e do baga?o de malte em rela??o aos valores percentuais de umidade, cinzas, extrato et?reo, prote?na bruta, a??cares sol?veis totais, amido, hemicelulose, celulose, lignina, fibra em detergente neutro e fibra em detergente ?cido. Com os resultados da caracteriza??o qu?mica p?de-se estimar uma produ??o potencial superior a 300 L de etanol/tonelada de cada res?duo. Para a otimiza??o do pr?-tratamento ?cido para remo??o da fra??o hemicelul?sica foi aplicada metodologia de Planejamento Fatorial atrav?s de Delineamento Composto Central Rotacional. O programa STATISTICA Vers?o 8.0 (Statsoft Inc., Tulsa,) foi utilizado para an?lise dos dados. A qualidade do ajuste da equa??o do modelo foi expressa pelo coeficiente de determina??o (R?) e sua signific?ncia estat?stica condicionada pelo teste-F. O material insol?vel recuperado ap?s o pr?-tratamento otimizado teve as fra??es hemicelul?sicas e de amido completamente removidas. O hidrolisado obtido em condi??es otimizadas de pr?-tratamento apresentou 15,7% de a??cares redutores para o baga?o de malte e pupunha e 5,9% de a??cares redutores para a torta de caro?o de algod?o. O tratamento dos hidrolisados com carv?o ativado removeu n?o menos que 58% dos compostos fen?licos a? presentes. Os testes de fermentabilidade com Saccharomyces cerevisiae e Pichia stipitis foram promissores. A fermenta??o com S. cerevisiae apresentou um YP/S de 0,30 para a torta de caro?o de algod?o e 0,37 para o baga?o de malte. Os rendimentos fementativos com P. stipitis variaram de 9% a 25% para os res?duos agroindustriais. Os dados experimentais indicam que a torta de caro?o de algod?o (Gossypium hirsutum L.) e baga?o malte com farinha de pupunha tem elevado potencial para a produ??o de bioetanol. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT Nowadays, one cannot ignore the need for developing technologies that support the demands for renewable energy. One of the pathways for production of renewable energy is the transformation of vegetal biomass to liquid fuels. The agro-industrial residues as raw materials are potential candidates for this new energy industry. Thus the aim of the present dissertation was to evaluate the potential of cottonseed cake and crushed malt with peach palm flour as raw materials for the production of second generation ethanol and optimize step of acid pretreatment of lignocellulosic material. Initially was determined the chemical composition of cotton seed cake and crushed malt in relation to the percentages of moisture, ash, ether extract, crude protein, total soluble sugars, starch, hemicellulose, cellulose, lignin, neutral detergent fiber and acid detergent fiber. With the results of chemical characterization it was possible to estimate a potential yield of more than 300L of ethanol / ton of each residue. For the optimization of acid pretreatment for hemicelluloses removal was applied a Central Composite Design. The software STATISTICA Version 8.0 (Statsoft Inc., Tulsa,) was used for data analysis. The goodness of fit of the model equation was expressed by the coefficient of determination (R?) and its statistical significance conditioned by F-test. The insoluble material recovered after the optimized pretreatment had starch and hemicellulose fractions completely removed. The hydrolyzate obtained under optimized conditions of pretreatment showed 15.7% of reducing sugars to the crushed malt and 5.9% of reducing sugars for cottonseed cake. The treatment of the hydrolysates with activated charcoal removed no less than 58% of the phenolic compounds present there. Fermentability tests with Saccharomyces cerevisiae and Pichia stipitis have been promising. Fermentation with S. cerevisiae showed a YP/S of 0.30 for cottonseed cake and 0.37 for the crushed malt. Fermentative yields with P. stipitis ranged from 9% to 25% for agro-industrial residues. The experimental data indicate that the cottonseed cake (Gossypium hirsutum L.) and crushed malt has a high potential for the production of bioethanol.

Submitted by Reginaldo Soares de Freitas (reginaldo.freitas@ufv.br) on 2016-09-05T18:53:01Z No. of bitstreams: 1 texto completo.pdf: 271370 bytes, checksum: c0dd5876aaa6de491bb7636fa5d32f47 (MD5) / Made available in DSpace on 2016-09-05T18:53:01Z (GMT). No. of bitstreams: 1 texto completo.pdf: 271370 bytes, checksum: c0dd5876aaa6de491bb7636fa5d32f47 (MD5) Previous issue date: 1998-02-09 / Fundação de Amparo à Pesquisa do Estado de Minas Gerais / Tridecan-2-ona (2-TD) e undecan-2-ona (2-UD), presentes no tomateiro- selvagem Lycopersicon hirsutum f. glabratum (PI 134417), atuam como principais fatores de resistência a diversas pragas do gênero Lycopersicon, nos EUA. Entretanto, apesar de o tomateiro-selvagem demonstrar resistência contra Tuta absoluta, os aleloquímicos 2-TD e 2-UD ainda não são comprovadamente considerados fatores de resistência. O presente trabalho objetivou obter dados sobre fatores de resistência química desse tomateiro, através da identificação dos componentes presentes no extrato hexânico das folhas de L. hirsutum f. glabratum, bem como avaliar sua toxicidade sobre T. absoluta. Outro objetivo foi determinar curvas de concentração-mortalidade dos padrões 2-TD e 2-UD. Sementes do tomateiro-selvagem foram plantadas na Horta Nova da Universidade Federal de Viçosa, em Viçosa, MG, e, aos 120 dias de idade, as folhas foram extraídas com hexano por seis horas. O hexano foi evaporado e o resíduo, fracionado por coluna cromatográfica de sílica-gel, usando-se solventes de polaridades diversas. As frações com composição química similar foram agrupadas em 29 subfrações e analisadas, por cromatógrafo, em fase gasosa/espectrometria de massas (CG/EM), tendo sido possível identificar 2-TD, 2-UD, trans-cariofileno, α-humuleno e outros compostos. Verificou-se que, em 8 das 29 subfrações, os principais componentes foram 2-TD e 2-UD e uma subfração continha trans-cariofileno e α-humuleno. A toxicidade de todas as 29 subfrações foi avaliada mediante o emprego de larvas de 2o ínstar de T. absoluta, verificando-se que 7 subfrações, das 8 que continham 2-TD e 2-UD, foram significativamente mais tóxicas do que as outras (Scott-Knott, p < 0,05). A partir desses resultados, bem como dos da literatura, pode-se concluir que 2-TD e 2-UD são os principais fatores de resistência a T. absoluta presentes no extrato hexânico de L. hirsutum f. glabratum. A partir de dados das curvas de concentração-mortalidade, verificou-se que 2-TD é mais tóxico que 2-UD e que ocorreram interações antagonistas e neutras de 2-UD sobre 2-TD, dependendo das suas concentrações. / Tridecan-2-ona (2-TD) and undecan-2-ona (2-UD) present in the wild tomato variety Lycopersicon hirsutum f. glabratum (PI 134417) were shown to be resistance factors against several Lycopersicon pests found in the USA. However, despite the fact that the variety L. hirsutum f. glabratum is known to be resistant to Tuta absoluta attack, 2-TD and 2-UD have not been conclusively shown to be the major resistance factors. The objective of this work was to obtain data on chemical resistance factors by identifying the chemical constituents of the hexane extract of L. hirsutum f. glabratum leaves and determining its toxicity against T. absoluta. Another objective was to determine the mortality-concentration curves of 2-TD and 2-UD standards and their mixtures. The seeds of L. hirsutum f. glabratum were planted in Horta Nova of the Universidade Federal de Viçosa and at 120 days, the leaves were extracted with hexane for 6 hours. The residue obtained after evaporation of the solvent was fractioned on a silica gel column using solvents of increasing polarities. Fractions of similar compositions (TLC) were combined to obtain a total of 29 subfractions which were analyzed by gas chromatography/mass spectrometry (GC/MS). The compounds 2-TD, 2-UD, trans-carophyllene and α-humulene were identified along with other compounds. Also, 8 of 29 subfractions contained 2-TD and 2-UD as the principal compounds while only one contained trans-carophyllene and α-humulene. The toxicity of all the 29 subfractions was evaluated using 2° instar T. absoluta larvae and it was found that 7 of 8 subfractions containing 2-TD and 2-UD were significantly more toxic than the other fractions (Scott-Knott test, p<0.05). Based on this study and others in the literature it appears that 2-TD and 2-UD are the principal resistance factors present in the hexane extract of L. hirsutum f. glabratum leaves against T.absoluta. The mortality- concentration data on 2-TD and 2-UD and in mixtures showed that 2-TD is more toxic than 2-UD and that antagonistic and neutral interactions of 2-UD on 2-TD occurred, depending upon their concentrations. / Não foi localizado o cpf do autor.

Submitted by ELIZABETH HARUNA KAZAMA null (bethkazama@hotmail.com) on 2016-04-05T19:43:23Z No. of bitstreams: 1 Dissertação_Elizabeth_Haruna_Kazama.pdf: 2591001 bytes, checksum: b6af06ca83c4e70605f88b2a36377803 (MD5) / Approved for entry into archive by Ana Paula Grisoto (grisotoana@reitoria.unesp.br) on 2016-04-06T19:15:35Z (GMT) No. of bitstreams: 1 kazama_eh_me_jabo.pdf: 2591001 bytes, checksum: b6af06ca83c4e70605f88b2a36377803 (MD5) / Made available in DSpace on 2016-04-06T19:15:35Z (GMT). No. of bitstreams: 1 kazama_eh_me_jabo.pdf: 2591001 bytes, checksum: b6af06ca83c4e70605f88b2a36377803 (MD5) Previous issue date: 2016-02-29 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O preço do algodão é determinado pela qualidade da fibra, que pode ser danificada no momento da colheita, gerando perdas qualitativas, além das perdas quantitativas de pluma não colhida, resultando em perda de produtividade. Neste trabalho foram realizados dois estudos que tiveram como objetivos: modelar a distribuição espacial e o mapeamento das perdas na colheita mecanizada do algodão em duas velocidades de colheita (5 km h-1 e 7 km h-1), (Experimento 1), e avaliar as perdas quali-quantitativas da fibra em cinco velocidades de colheita, 5, 6, 7, 8 e 9 km h -1 (Experimento 2). Os experimentos foram realizados em dois municípios do estado do Mato Grosso, Brasil, sendo um sob delineamento de malha regular, demarcados 64 pontos amostrais com 5,25 ha cada, e outro sob delineamento de blocos inteiramente casualizados, utilizando a estatística clássica, sendo cinco velocidades de colheita e quatro repetições. No primeiro experimento, a maior extensão das perdas no terço inferior da planta ocorre no sentido das linhas de semeadura e deslocamento da colhedora e, além disso, as perdas na planta possuem relação com a população de plantas. As perdas possuem dependência espacial, demonstrando que possui relação com a planta. No segundo experimento, as perdas no solo, na planta e a qualidade da fibra não foram afetadas pelas velocidades analisadas. / The price of the cotton is determined by the quality of the fiber, which can be damaged at time of harvest, generating qualitative loss, in addition to feather quantitative losses, resulting loss of productivity. This work carried out two studies to the following objectives: to model the spatial distribution and the losses mapping in mechanized cotton harvesting in two harvest speed (5 km h-1 and 7 km h-1), (Experiment 1), and evaluating the quantitative and qualitative losses of five-speed fiber harvesting, 5, 6, 7, 8 and 9 km h-1 (Experiment 2). The experiments were conducted in two cities in the state of Mato Grosso, Brazil, one under regular mesh design, marked 64 sample points, with 5.25 ha, in each field, the other was randomized in complete block, using classical statistics, with five-speed harvesting and four replications. In the first experiment, the largest extent of the losses in the lower third of the plant occurs in the direction of the planting lines and displacement of the harvester in addition, losses in the plant are related to the population of plants. Cotton losses have spatial dependence demonstrating that It has relation with the the plant. In the second experiment, the losses in soil, plant and fiber quality were not affected by the analyzed speeds.