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Histone H1 and the evolution of protaminesLewis, John David MacLean 21 April 2017 (has links)
It has been proposed that protamines have evolved vertically from an ancestral histone
H1. My research has concentrated mainly on the investigation of this proposal by
characterizing the sperm nuclear basic proteins (SNBPs) and their genes from a diverse
range of organisms which employ histones, protamines, or protamine-like proteins to
achieve sperm chromatin compaction. The complete gene sequences were obtained for
the large histone H1-related protamine-like PL-I of the bivalve mollusc Spisula
solidissima, the small protamine-like PL-III protein of related bivalve Mytilus
californianus, and the protamine of the squid, Loligo opalescens, which is the first
invertebrate protamine gene to be characterized. In addition, a full-length cDNA from the
novel protamine and histone H1-related sperm nuclear protein of the primitive chordate,
Styela montereyensis, was isolated and characterized. This genetic data, beyond providing
valuable information on the regulation and organization of the heterogeneous family of
SNBPs, has provided unequivocal support to the hypothesis that the chromatin-condensing
protamines of the sperm have evolved from the chromatin-condensing
histones of somatic cells. This has in turn allowed a more accurate tracing of the origin of
histone H1, protamines and protamine-like proteins in both the protostomes and
deuterostomes. / Graduate
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Linker histone/DNA interactions : in vitro studiesHassan, Ahmed H. 17 April 1997 (has links)
By studying the linker histone/DNA complex in vitro, our goal was to
gain further insight into the interaction of these histones in chromatin structure,
which, in turn, helps us in better understanding critical biological processes such
as replication, transcription, and recombination. In direct competition
experiments, we have confirmed previous results of the preference of the linker
histones in binding to superhelical DNA over linear or nicked circular DNA
forms. This binding of linker histones to DNA supercoils in the presence of DNA
competitors was examined at varying histone/DNA ratios and different ionic
strengths. It was demonstrated that: 1) With increasing H1/DNA ratios, the
electrophoretic mobility of the H1/supercoiled DNA complex decreases. 2) With
increasing ionic strengths, the mobility of this complex increases. The presence of
more protein bound to superhelical DNA explains the first observation. The
second observation, however, is explained by a reduction in the amount of
protein bound to DNA as well as a change in the conformation of the complex at
higher salt concentrations.
The conformational change of the superhelical DNA upon linker histone
binding was then investigated. The effect of linker histone binding on the
accessibility of sites on superhelical plasmids to single-strand-specific nucleases
(P1 or S1) was studied first. The results show protection of preexisting P1- or S1- sensitive sites at low to moderate linker histone/DNA ratios as well as the appearance of new susceptible sites at higher ratios. The protection of singlestrand-specific nuclease-sensitive sites can be explained by a change in the superhelical torsion of the plasmid as a result of linker histone binding. Furthermore, the interaction of the C-terminal domain of the linker histones with AT-rich sites on DNA could (by destabilizing B-DNA structure) make the sites susceptible to nuclease cleavage. This explains the appearance of new susceptible sites at higher linker histone levels.
These changes in the pattern of cleavage by nucleases with increasing linker histone/DNA ratios were further studied by investigating the effect of linker histones on superhelical plasmids upon binding, looking for changes in the linking number of the plasmid DNA. Two classical assays (the topoisomerase I-mediated relaxation assay and the ligase-mediated supercoiling assay) were performed. The results clearly indicate that the linker histones unwind superhelical DNA, with the unwinding angle being about 8�� per histone H1 molecule bound to DNA. Even though this unwinding angle is small relative to the unwinding effect of other proteins, it is crucial to consider this effect in the studies of chromatin fiber structure. / Graduation date: 1997
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Chicken histone gene organisation /DAndrea, Richard James. January 1985 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Biochemistry, 1985. / Includes bibliographical references (leaves 155-177).
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Structure and distribution of chicken histone H2A.F /Whiting, Jennifer Anne. January 1988 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Biochemistry, 1988. / Includes bibliographical references (leaves 129-139).
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TEMPORAL RELATIONSHIPS BETWEEN HISTONE SYNTHESIS AND MODIFICATION AND OTHER METABOLIC EVENTS DURING THE GERMINATION OF VICIA FABA EMBRYOSStanley, Wayne Scott, 1945- January 1972 (has links)
No description available.
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The structural role of histone H2A variants in chromatin functionAbbott, D. Wade 10 April 2008 (has links)
Chromatin is a highly dynamic complex that facilitates the structural transitions required for specific gene expression. An emerging player in the regulation of such chromatin functions are histone H2A variants. These proteins alter the histone-histone and histone-DNA interactions within the nucleosome to generate specialized nucleosomes with dedicated function. In this regard, it is quite possible that the Cterminal tails of H2A proteins confer a direct structural effect by altering the stability or folding potential of nucleosome arrays. This thesis addresses this issue by presenting the biophysical characterization of chromatin particles reconstituted with three different histone H2A variants. H2A.2, an essential protein, destabilizes the nucleosome and reduces the salt-dependent folding propensity of chromatin. H2A-Bbd, a histone variant exclusive to transcriptionally active domains, destabilizes the nucleosome and is more mobile within the nucleus. MacroH2A, which is believed to be involved in transcriptional repression, stabilizes the nucleosome and displays a C-terminal domain that is enriched in a-helix and adopts a globular conformation. Using irnmunochemical analysis it was determined that macroH2A is only found in subphylum vertebrata, is evenly distributed throughout autosomal chromatin at various levels of structure, and has a mutually exclusive relationship with histone HI. Interestingly, the ADP-ribosylation of macroH2A results in a stoichiometric decrease from two copies to one copy of macroH2A in a specific nucleosome, suggesting that the post-translational modification of histone variants may directly regulate nucleosome integrity
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On the location of the linker histones and the linker DNA in the 30 nm fiber of chromatinLeuba, Sanford H. 07 May 1993 (has links)
Understanding the structure of the 30 nm fiber in chromatin is relevant to understanding eukaryotic replication and transcription. The major controversy among the models of the fiber concerns the disposition of the linker DNA, the DNA between adjacent nucleosomes, and the location of the linker histones. To determine if the location of the linker histones and the linker DNA was internal or external, chromatin was digested with immobilized proteases and nucleases. The chromatin was probed either in a low salt extended 10 nm fiber of nucleosomes or in progressive compactions (addition of increasing amounts of salt) to form a condensed (30 nm) fiber.
Digestion experiments performed on linker histones either in chicken erythrocyte chromatin, or free in solution or bound in mononucleosomes revealed: (1) Histone H5 is more protected than histone H1 in the fiber; (2) The N-and C-terminal portions of H1 do not change their
accessibility upon compaction of the fiber; the tails of H5, however, become significantly internalized in the 30 nm fiber; (3) phenylalanine in the globular domain of both H1 and H5 is inaccessible both in the fiber and in mononucleosomes. Sedimentation velocity measurements demonstrate that the conformation of the fiber at all its different condensation states is highly sensitive to cuts in even a few of the linker histone molecules.
The structure of these chromatin fibers has also been probed using micrococcal nuclease, both membrane-immobilized and free in solution, under extremely mild digestion conditions. The linker DNA is almost completely protected against digestion in the 30 nm fibers, whereas it is readily accessible in the more extended structures, independent of whether immobilized or free enzyme is employed. To circumvent complications due to the sensitivity of the enzyme to the salt concentration, control experiments were performed in which chromatin fibers were glutaraldehyde-fixed under different ionic conditions and then digested in low salt. The results were very similar to the above. Experiments with fibers of intermediate degree of condensation revealed a direct relationship between the degree of compaction and the resistance of linker DNA to digestion. These results support models for chromatin structure in which access to the linkers is limited by local steric hindrance, rather than by internalization in the core of the fibers. / Graduation date: 1993
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Towards the understanding of the function of the histone "tails" with respect to the structure, stability, and function of chromatinDong, Feng 13 July 1990 (has links)
By using immobilized trypsin, I have been able to
prepare well-defined, stable trypsinized nucleosomes. The
difficulties of lacking of control in the extent of
trypsinization, which were encountered in previous studies
with the use of free trypsin, have been eliminated. The
nucleosomes and oligonucleosomes prepared by immobilized
trypsin are suitable for biochemical and biophysical
studies to analyze the function of the histone N-terminal
regions ("tails"), which are removed by trypsin treatment,
on chromatin structure and stability.
Studies were first conducted using the trypsinized
nucleosome core particles to examine the role of the
histone tails in the stabilization of the nucleosome core
particle. While it was found that these tails have little
effect on either the nucleosome dissociation or the
conformational transition in salt, they play a very
important role in determining thermal stability of the
nucleosome. The differential effects of selective removal
of these tails also provided more insight about histone-DNA
interactions in the nucleosome core particle.
Experiments have also been carried out to investigate
the change in structure and hydration of nucleosome core
particles which may be associated with the salt-dependent
conformational transition. Changes in the tertiary
structures are suggested to be responsible for the salt-dependent
transition.
Roles of the histone tails in determination of
nucleosome positions along specific DNA sequences were
examined by analysis of nucleosome positioning on a
specific eukaryotic gene sequence (Lytechinus Variegatus 5S
rRNA gene) after in vitro nucleosome reconstitution with
native and trypsinized histone octamers. Data obtained
indicate that the histone tails are not required for
nucleosome positioning. Results also seem to restrict the
portions of histones which are responsible for determining
nucleosome positions to the globular regions of (H3/H4)₂
tetramer, and possibly H2B. Studies with different DNA
templates strongly suggest that the most important
determinants of nucleosome positioning are the mechanical
properties (such as bending and flexibility) of the DNA
molecule.
Taking together, it seems that the N-terminal tails of
the histones may play roles in stabilizing both nucleosome
structure and the higher-order structure of chromatin. / Graduation date: 1991
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Biochemical characterization of a hat1p-containing histone acetyltransferase complexAi, Xi, January 2004 (has links)
Thesis (Ph. D.)--Ohio State University, 2004. / Title from first page of PDF file. Document formatted into pages; contains xv1, 151 p.; also includes graphics. Includes abstract and vita. Advisor: Mark Parthun, Dept. of Biochemistry. Includes bibliographical references (p. 138-151).
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Genetic and genomic studies of histone H3 methylation and acetylationJin, Yi. January 2008 (has links) (PDF)
Thesis (Ph. D.)--Washington State University, December 2008. / Title from PDF title page (viewed on Feb. 18, 2010). "Department of Molecular Plant Sciences." Includes bibliographical references.
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