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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Histone H1 and the evolution of protamines

Lewis, John David MacLean 21 April 2017 (has links)
It has been proposed that protamines have evolved vertically from an ancestral histone H1. My research has concentrated mainly on the investigation of this proposal by characterizing the sperm nuclear basic proteins (SNBPs) and their genes from a diverse range of organisms which employ histones, protamines, or protamine-like proteins to achieve sperm chromatin compaction. The complete gene sequences were obtained for the large histone H1-related protamine-like PL-I of the bivalve mollusc Spisula solidissima, the small protamine-like PL-III protein of related bivalve Mytilus californianus, and the protamine of the squid, Loligo opalescens, which is the first invertebrate protamine gene to be characterized. In addition, a full-length cDNA from the novel protamine and histone H1-related sperm nuclear protein of the primitive chordate, Styela montereyensis, was isolated and characterized. This genetic data, beyond providing valuable information on the regulation and organization of the heterogeneous family of SNBPs, has provided unequivocal support to the hypothesis that the chromatin-condensing protamines of the sperm have evolved from the chromatin-condensing histones of somatic cells. This has in turn allowed a more accurate tracing of the origin of histone H1, protamines and protamine-like proteins in both the protostomes and deuterostomes. / Graduate
2

Linker histone/DNA interactions : in vitro studies

Hassan, Ahmed H. 17 April 1997 (has links)
By studying the linker histone/DNA complex in vitro, our goal was to gain further insight into the interaction of these histones in chromatin structure, which, in turn, helps us in better understanding critical biological processes such as replication, transcription, and recombination. In direct competition experiments, we have confirmed previous results of the preference of the linker histones in binding to superhelical DNA over linear or nicked circular DNA forms. This binding of linker histones to DNA supercoils in the presence of DNA competitors was examined at varying histone/DNA ratios and different ionic strengths. It was demonstrated that: 1) With increasing H1/DNA ratios, the electrophoretic mobility of the H1/supercoiled DNA complex decreases. 2) With increasing ionic strengths, the mobility of this complex increases. The presence of more protein bound to superhelical DNA explains the first observation. The second observation, however, is explained by a reduction in the amount of protein bound to DNA as well as a change in the conformation of the complex at higher salt concentrations. The conformational change of the superhelical DNA upon linker histone binding was then investigated. The effect of linker histone binding on the accessibility of sites on superhelical plasmids to single-strand-specific nucleases (P1 or S1) was studied first. The results show protection of preexisting P1- or S1- sensitive sites at low to moderate linker histone/DNA ratios as well as the appearance of new susceptible sites at higher ratios. The protection of singlestrand-specific nuclease-sensitive sites can be explained by a change in the superhelical torsion of the plasmid as a result of linker histone binding. Furthermore, the interaction of the C-terminal domain of the linker histones with AT-rich sites on DNA could (by destabilizing B-DNA structure) make the sites susceptible to nuclease cleavage. This explains the appearance of new susceptible sites at higher linker histone levels. These changes in the pattern of cleavage by nucleases with increasing linker histone/DNA ratios were further studied by investigating the effect of linker histones on superhelical plasmids upon binding, looking for changes in the linking number of the plasmid DNA. Two classical assays (the topoisomerase I-mediated relaxation assay and the ligase-mediated supercoiling assay) were performed. The results clearly indicate that the linker histones unwind superhelical DNA, with the unwinding angle being about 8�� per histone H1 molecule bound to DNA. Even though this unwinding angle is small relative to the unwinding effect of other proteins, it is crucial to consider this effect in the studies of chromatin fiber structure. / Graduation date: 1997
3

Chicken histone gene organisation /

DAndrea, Richard James. January 1985 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Biochemistry, 1985. / Includes bibliographical references (leaves 155-177).
4

Structure and distribution of chicken histone H2A.F /

Whiting, Jennifer Anne. January 1988 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Biochemistry, 1988. / Includes bibliographical references (leaves 129-139).
5

TEMPORAL RELATIONSHIPS BETWEEN HISTONE SYNTHESIS AND MODIFICATION AND OTHER METABOLIC EVENTS DURING THE GERMINATION OF VICIA FABA EMBRYOS

Stanley, Wayne Scott, 1945- January 1972 (has links)
No description available.
6

The structural role of histone H2A variants in chromatin function

Abbott, D. Wade 10 April 2008 (has links)
Chromatin is a highly dynamic complex that facilitates the structural transitions required for specific gene expression. An emerging player in the regulation of such chromatin functions are histone H2A variants. These proteins alter the histone-histone and histone-DNA interactions within the nucleosome to generate specialized nucleosomes with dedicated function. In this regard, it is quite possible that the Cterminal tails of H2A proteins confer a direct structural effect by altering the stability or folding potential of nucleosome arrays. This thesis addresses this issue by presenting the biophysical characterization of chromatin particles reconstituted with three different histone H2A variants. H2A.2, an essential protein, destabilizes the nucleosome and reduces the salt-dependent folding propensity of chromatin. H2A-Bbd, a histone variant exclusive to transcriptionally active domains, destabilizes the nucleosome and is more mobile within the nucleus. MacroH2A, which is believed to be involved in transcriptional repression, stabilizes the nucleosome and displays a C-terminal domain that is enriched in a-helix and adopts a globular conformation. Using irnmunochemical analysis it was determined that macroH2A is only found in subphylum vertebrata, is evenly distributed throughout autosomal chromatin at various levels of structure, and has a mutually exclusive relationship with histone HI. Interestingly, the ADP-ribosylation of macroH2A results in a stoichiometric decrease from two copies to one copy of macroH2A in a specific nucleosome, suggesting that the post-translational modification of histone variants may directly regulate nucleosome integrity
7

On the location of the linker histones and the linker DNA in the 30 nm fiber of chromatin

Leuba, Sanford H. 07 May 1993 (has links)
Understanding the structure of the 30 nm fiber in chromatin is relevant to understanding eukaryotic replication and transcription. The major controversy among the models of the fiber concerns the disposition of the linker DNA, the DNA between adjacent nucleosomes, and the location of the linker histones. To determine if the location of the linker histones and the linker DNA was internal or external, chromatin was digested with immobilized proteases and nucleases. The chromatin was probed either in a low salt extended 10 nm fiber of nucleosomes or in progressive compactions (addition of increasing amounts of salt) to form a condensed (30 nm) fiber. Digestion experiments performed on linker histones either in chicken erythrocyte chromatin, or free in solution or bound in mononucleosomes revealed: (1) Histone H5 is more protected than histone H1 in the fiber; (2) The N-and C-terminal portions of H1 do not change their accessibility upon compaction of the fiber; the tails of H5, however, become significantly internalized in the 30 nm fiber; (3) phenylalanine in the globular domain of both H1 and H5 is inaccessible both in the fiber and in mononucleosomes. Sedimentation velocity measurements demonstrate that the conformation of the fiber at all its different condensation states is highly sensitive to cuts in even a few of the linker histone molecules. The structure of these chromatin fibers has also been probed using micrococcal nuclease, both membrane-immobilized and free in solution, under extremely mild digestion conditions. The linker DNA is almost completely protected against digestion in the 30 nm fibers, whereas it is readily accessible in the more extended structures, independent of whether immobilized or free enzyme is employed. To circumvent complications due to the sensitivity of the enzyme to the salt concentration, control experiments were performed in which chromatin fibers were glutaraldehyde-fixed under different ionic conditions and then digested in low salt. The results were very similar to the above. Experiments with fibers of intermediate degree of condensation revealed a direct relationship between the degree of compaction and the resistance of linker DNA to digestion. These results support models for chromatin structure in which access to the linkers is limited by local steric hindrance, rather than by internalization in the core of the fibers. / Graduation date: 1993
8

Towards the understanding of the function of the histone "tails" with respect to the structure, stability, and function of chromatin

Dong, Feng 13 July 1990 (has links)
By using immobilized trypsin, I have been able to prepare well-defined, stable trypsinized nucleosomes. The difficulties of lacking of control in the extent of trypsinization, which were encountered in previous studies with the use of free trypsin, have been eliminated. The nucleosomes and oligonucleosomes prepared by immobilized trypsin are suitable for biochemical and biophysical studies to analyze the function of the histone N-terminal regions ("tails"), which are removed by trypsin treatment, on chromatin structure and stability. Studies were first conducted using the trypsinized nucleosome core particles to examine the role of the histone tails in the stabilization of the nucleosome core particle. While it was found that these tails have little effect on either the nucleosome dissociation or the conformational transition in salt, they play a very important role in determining thermal stability of the nucleosome. The differential effects of selective removal of these tails also provided more insight about histone-DNA interactions in the nucleosome core particle. Experiments have also been carried out to investigate the change in structure and hydration of nucleosome core particles which may be associated with the salt-dependent conformational transition. Changes in the tertiary structures are suggested to be responsible for the salt-dependent transition. Roles of the histone tails in determination of nucleosome positions along specific DNA sequences were examined by analysis of nucleosome positioning on a specific eukaryotic gene sequence (Lytechinus Variegatus 5S rRNA gene) after in vitro nucleosome reconstitution with native and trypsinized histone octamers. Data obtained indicate that the histone tails are not required for nucleosome positioning. Results also seem to restrict the portions of histones which are responsible for determining nucleosome positions to the globular regions of (H3/H4)₂ tetramer, and possibly H2B. Studies with different DNA templates strongly suggest that the most important determinants of nucleosome positioning are the mechanical properties (such as bending and flexibility) of the DNA molecule. Taking together, it seems that the N-terminal tails of the histones may play roles in stabilizing both nucleosome structure and the higher-order structure of chromatin. / Graduation date: 1991
9

Biochemical characterization of a hat1p-containing histone acetyltransferase complex

Ai, Xi, January 2004 (has links)
Thesis (Ph. D.)--Ohio State University, 2004. / Title from first page of PDF file. Document formatted into pages; contains xv1, 151 p.; also includes graphics. Includes abstract and vita. Advisor: Mark Parthun, Dept. of Biochemistry. Includes bibliographical references (p. 138-151).
10

Genetic and genomic studies of histone H3 methylation and acetylation

Jin, Yi. January 2008 (has links) (PDF)
Thesis (Ph. D.)--Washington State University, December 2008. / Title from PDF title page (viewed on Feb. 18, 2010). "Department of Molecular Plant Sciences." Includes bibliographical references.

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