The aim of this diploma thesis was describe and evaluate individual genotypes of genus Rheum L., which are planted on field of Horticulture Faculty MENDELU in Lednice. Descriptors were evaluated with descriptor list, including laboratory examination of content of ascorb acid, oxalic acid, refractometric dry matter, nitrates (NO3), antioxidant capacity (TAC) and dry matter. Purpose of this work is take care of present collection of genotypes and increase its number with additional promising genotypes. Maintenance of gene pool of rhubarb is needed for preservation not just original genetic resources but also for maintaining overall biodiversity.