MPH / Department of Public Health / Water quality of drinking water has been linked to good health outcomes across the world. The aim of this study was to assess physico-chemical, bacteriological, community practices regarding collection and storage of water and evaluation of human health risk characteristics of drinking water supplied by the government to Lufule village in Thulamela municipality, Limpopo Province, South Africa A cross-sectional study was conducted using questionnaires and interviews to determine drinking water handling practices and levels of contamination between the source and point-of-use at household. Assessment of water quality was carried out on 114 samples from selected sampling points using scientifically approved protocols. Total coliform was determined in 62.5% and 87.5% of the samples during the dry and wet seasons respectively. Similarly, E. coli was determined in 10.4 % and 13.2% in the dry and wet seasons, respectively. Trace metals levels in the drinking water samples were analysed and were within permissible range of both SANS and WHO. The calculated non-carcinogenic effects using hazard quotient toxicity potential, cumulative hazard index and chronic daily intake of drinking water through ingestion pathways were less than one unity, which showed that consumption of the water could pose little or no significant health risk. The results of this research suggest that lead has the potential of cancer risk to the residents through the cumulative ingestion in the drinking water samples of the studied area. Therefore, precaution needs to be taken to avoid potential risk of people in Lufule area especially, children. / NRF