Dissolved compounds and ions, including mineral elements and products of microbial metabolism, are present in many cheeses in relatively high concentrations. These dissolved substances may precipitate from the aqueous phase of cheese to form sparingly soluble crystals that can impart a crunchy, gritty, or sandy texture on the cheese. In the present work, optical and diffractometric methods were optimized for use with cheese samples to identify crystal phases in several cheese varieties. These techniques, which included powder X-ray diffractometry (PXRD), single crystal X-ray diffractometry (SCXRD), and petrographic microscopy (PM) have traditionally been used on geological specimens that are quite different from the cheese samples used in the present study. Nonetheless, these techniques were successfully used to gain valuable insight into crystal development in cheese. Powder X-ray diffractometry was optimized to minimize the occurrence of artifacts that may occur due to the high water content and low crystallinity of some cheese samples. The use of enhanced sample preparation techniques facilitated the identification of organic and inorganic crystal phases such as tyrosine, leucine, brushite (CaHPO4·2H2O), and calcite (CaCO3) in hard and soft cheeses. SCXRD was used to determine the crystal structures of ikaite and struvite, which had been tentatively identified in washed-rind cheese using PXRD. PM was used to observe morphological and optical properties of crystals in white mold cheese and washed-rind cheese. In two subsequent aging studies, PXRD was used to determine the approximate timing of crystal nucleation in the rinds of white mold cheese and washed-rind cheese. These observations were paired with inductively coupled plasma-atomic emission spectroscopy (ICP-AES) data to demonstrate that the onset of crystallization in the rinds coincided with a diffusion phenomenon in which mineral ions diffused from the center of the cheese and became concentrated in the rind. PM observations demonstrated that maximum crystal size in the rinds generally increased as aging progressed. These observations will be useful in future work that investigates the impact of crystallization on sensory properties of cheese.

Aligned ikaite porphyroblasts have been replaced by calcite with a maximum volume decrease of ~88% in Neoproterozoic Dalradian metalimestone exposed near Ballygrant on Islay, Scotland. Microscopic and structural analyses reveal that the ikaite minerals formed before deformation, at a time when Scotland was located at the lower latitudes, thus indicating a cold climate at the time of formation. The Ballygrant metalimestone underlies a ~900 meter thick glacial deposit that has previously been linked to the ‘Snowball Earth’ hypothesis. The discovery of these ikaite pseudomorphs below the glacial deposit points toward a relatively slow cooling of the climate near the equator reflecting a transition toward a ‘normal’ glacial period and thus refutes a suggested ‘Snowball Earth’ event, which is reported to reflect a more abrupt switch over from warm to cold climate in the sediment record.

Manito Lake is a large, perennial, Na-SO4 dominated hypersaline lake located in the northern Great Plains of western Canada. Significant water level decrease over the past several decades has lead to reduction in volume and surface area. Today, the lake is 15% of its mid -20th century volume and 46% of its former area. This decrease in water level has exposed large areas of nearshore microbialites. These organosedimentary structures have various external morphologies, vary in mineralogical composition, and show a variety of internal fabrics from finely laminated to massive and clotted. These features range from small, mm-scale, finely laminated encrustations to large, reef-like structures up to 5 m high and over 500 m long. Although there is relatively little consistent lateral variability in terms of morphology, the structures do vary significantly with elevation in the basin. Petrographic evidence confirms a strong biological involvement in the formation of these structures. Nonetheless, inorganic and trapping mechanisms may also play a role. Dolomite, aragonite, and calcite are the most commonly found minerals in these structures, however, monohydrocalcite, magnesian calcite, hydromagnesite, dypingite, and nesquehonite are also present. The calcite is a pseudomorph after ikaite, which forms an open porous dendritic and shrub-like fabric, comprising the interiors of massive shoreline microbialite mounds and pinnacles. These ikaite pseudomorphs are encased in millimeter to centimeter-scale laminated dolomite-aragonite rinds. Radiocarbon dating and stable isotope analysis have indicated microbialite formation began about 2200 yBP in a shallow, productive, saline and cold lake. Over the next 900 years, the microbialites record a transgressing lake in a cool climate, which corresponds to a period not previously documented in this region but is referred to as the Dark Ages Cold Period, which has been documented in other parts of the Northern Hemisphere. This is followed by 500 years of warmer conditions coinciding with the Medieval Climate Anomaly. Starting about 600 years ago the lake experienced a dramatic decrease in level resulting in formation of extensive carbonate pavements, cemented siliciclastics, rinds, and coatings.

Manito Lake is a large, perennial, Na-SO4 dominated hypersaline lake located in the northern Great Plains of western Canada. Significant water level decrease over the past several decades has lead to reduction in volume and surface area. Today, the lake is 15% of its mid -20th century volume and 46% of its former area. This decrease in water level has exposed large areas of nearshore microbialites. These organosedimentary structures have various external morphologies, vary in mineralogical composition, and show a variety of internal fabrics from finely laminated to massive and clotted. These features range from small, mm-scale, finely laminated encrustations to large, reef-like structures up to 5 m high and over 500 m long. Although there is relatively little consistent lateral variability in terms of morphology, the structures do vary significantly with elevation in the basin. Petrographic evidence confirms a strong biological involvement in the formation of these structures. Nonetheless, inorganic and trapping mechanisms may also play a role. Dolomite, aragonite, and calcite are the most commonly found minerals in these structures, however, monohydrocalcite, magnesian calcite, hydromagnesite, dypingite, and nesquehonite are also present. The calcite is a pseudomorph after ikaite, which forms an open porous dendritic and shrub-like fabric, comprising the interiors of massive shoreline microbialite mounds and pinnacles. These ikaite pseudomorphs are encased in millimeter to centimeter-scale laminated dolomite-aragonite rinds. Radiocarbon dating and stable isotope analysis have indicated microbialite formation began about 2200 yBP in a shallow, productive, saline and cold lake. Over the next 900 years, the microbialites record a transgressing lake in a cool climate, which corresponds to a period not previously documented in this region but is referred to as the Dark Ages Cold Period, which has been documented in other parts of the Northern Hemisphere. This is followed by 500 years of warmer conditions coinciding with the Medieval Climate Anomaly. Starting about 600 years ago the lake experienced a dramatic decrease in level resulting in formation of extensive carbonate pavements, cemented siliciclastics, rinds, and coatings.