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1	DEVELOPING A DEEP LEARNING PIPELINE TO AUTOMATICALLY ANNOTATE GOLD PARTICLES IN IMMUNOELECTRON MICROSCOPY IMAGES Unknown Date (has links) Machine learning has been utilized in bio-imaging in recent years, however as it is relatively new and evolving, some researchers who wish to utilize machine learning tools have limited access because of a lack of programming knowledge. In electron microscopy (EM), immunogold labeling is commonly used to identify the target proteins, however the manual annotation of the gold particles in the images is a time-consuming and laborious process. Conventional image processing tools could provide semi-automated annotation, but those require that users make manual adjustments for every step of the analysis. To create a new high-throughput image analysis tool for immuno-EM, I developed a deep learning pipeline that was designed to deliver a completely automated annotation of immunogold particles in EM images. The program was made accessible for users without prior programming experience and was also expanded to be used on different types of immuno-EM images. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2020. / FAU Electronic Theses and Dissertations Collection Electron microscopy Immunogold labeling Image analysis Deep learning
2	Ultrastructural Characterization of The Output of VIP Expressing Interneurons in Mouse Barrel Cortex Zhou, Xiaojuan 15 May 2017 (has links) No description available. 570 Cortical circuit Electron microscopy GABA Immunogold labeling Vasoactive intestinal polypeptide Biologie (PPN619462639)
3	Monitoring von Membranen und membrangebundenen Dehydrogenasen in Essigsäurebakterien / Monitoring of membranes and membrane-bound dehydrogenases in acetic acid bacteria Kokoschka, Sebastian 21 October 2013 (has links) No description available. 570 Mikrobiologie Gluconobacter oxydans Intracytoplasmatische Membranen Membrangebundene Dehydrogenasen Transmissionselektronenmikroskopie Immunogoldmarkierung Microbiology Gluconobacter oxydans Intracytoplasmic membranes Membrane-bound dehydrogenases Transmission electron microscopy Immunogold-labeling Biologie (PPN619462639)
4	Ultrastructural and Histochemical Characterization of the Zebra Mussel Adhesive Apparatus Farsad, Nikrooz 06 April 2010 (has links) Since their accidental introduction into the Great Lakes in mid- to late-1980s, the freshwater zebra mussels, Dreissena polymorpha, have colonized most lakes and waterways across eastern North America. Their rapid spread is partly attributed to their ability to tenaciously attach to hard substrates via an adhesive apparatus called the byssus, resulting in serious environmental and economic impacts. A detailed ultrastructural study of the bysuss revealed a 10 nm adhesive layer at the attachment interface. Distributions of the main adhesive amino acid, 3,4-dihydroxyphenylalanine (DOPA), and its oxidizing (cross-linking) enzyme, catechol oxidase, were determined histochemically. It was found that, upon aging, DOPA levels remained high in the portion of the byssus closest to the interface, consistent with an adhesive role. In contrast, reduced levels of DOPA corresponded well with high levels of catechol oxidase in the load-bearing component of the byssus, presumably forming cross-links and increasing the cohesive strength. Zebra Mussel Dreissena polymorpha byssus ultrastructure bioadhesive adhesive 3,4-dihydroxyphenylalanine DOPA TEM transmission electron microscopy bio-adhesive catechol oxidase immunogold labeling histochemistry histochemical methods adhesive plaque cryoultramicrotomy TEM biological sample preparation 0794 0487 0306
5	Ultrastructural and Histochemical Characterization of the Zebra Mussel Adhesive Apparatus Farsad, Nikrooz 06 April 2010 (has links) Since their accidental introduction into the Great Lakes in mid- to late-1980s, the freshwater zebra mussels, Dreissena polymorpha, have colonized most lakes and waterways across eastern North America. Their rapid spread is partly attributed to their ability to tenaciously attach to hard substrates via an adhesive apparatus called the byssus, resulting in serious environmental and economic impacts. A detailed ultrastructural study of the bysuss revealed a 10 nm adhesive layer at the attachment interface. Distributions of the main adhesive amino acid, 3,4-dihydroxyphenylalanine (DOPA), and its oxidizing (cross-linking) enzyme, catechol oxidase, were determined histochemically. It was found that, upon aging, DOPA levels remained high in the portion of the byssus closest to the interface, consistent with an adhesive role. In contrast, reduced levels of DOPA corresponded well with high levels of catechol oxidase in the load-bearing component of the byssus, presumably forming cross-links and increasing the cohesive strength. Zebra Mussel Dreissena polymorpha byssus ultrastructure bioadhesive adhesive 3,4-dihydroxyphenylalanine DOPA TEM transmission electron microscopy bio-adhesive catechol oxidase immunogold labeling histochemistry histochemical methods adhesive plaque cryoultramicrotomy TEM biological sample preparation 0794 0487 0306

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