Follicular antral oxygen tension is thought to influence subsequent oocyte developmental competence. Despite this, in vitro maturation (IVM) is routinely performed in either 5 or 20% oxygen and while low oxygen has been shown to be beneficial to embryo development in many species, the effects of altering oxygen concentration during IVM have not been adequately investigated. Here we investigated the effects of a range of oxygen concentrations (2, 5, 10 & 20% oxygen) during IVM of mouse oocytes on a range of oocyte and embryonic parameters as well as fetal/placental outcome measures and cumulus cell gene expression. While common short term measures of oocyte developmental competence such as maturation, fertilisation, and embryonic development rates were not affected over the range of oxygen levels used, more in depth investigations found several striking differences. Following IVM at 5% oxygen, the oocyte mitochondria were found to have altered patterns of both membrane potential (a measure of mitochondrial activity) and distribution suggesting altered oocyte metabolism. Following IVF, the cellular make up of embryos was investigated. In blastocysts derived from low IVM oxygen (2%) there was found to be an increased number of trophectoderm cells, an increased level of apoptosis (although this was not of sufficient magnitude to account for the cell number difference) and more cells positive for both Cdx2 and Oct4 (markers of trophectoderm and inner cell mass cell types respectively) suggesting a less differentiated cell type. Furthermore, following embryo transfer, the ability of the embryos to implant or develop was not altered by IVM oxygen concentration; however, fetal and placental weights were reduced in the 5% oxygen group. Cumulus cell gene expression was also examined and was found to be altered both across IVM oxygen treatment groups and when compared to cells isolated from in vivo derived complexes. This change in gene expression elucidates some of the many ways in which oxygen concentration during IVM may be affecting the cumulus-oocyte complex (COC) and its future development. Together, this data highlights the importance of looking past common outcome measures when determining the effects of IVM culture conditions. The results of this study also suggest that while IVM oxygen concentration contributes to the perturbing nature of current IVM systems, it is only one of many constituents that require proper investigation, understanding and optimisation. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1368831 / Thesis (Ph.D.) -- University of Adelaide, School of Paediatrics and Reproductive Health, 2009

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Univ., Diss., 2006--Giessen

Universiẗat, Diss., 2006--Giessen.

Tübingen, Univ., Diss., 2007.

Thesis (M. Phil.)--University of Hong Kong, 2007. / Title proper from title frame. Also available in printed format.

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Thesis (doctoral)--Universiẗat, Jena, 2003.