In 1994 and 1995, seven cases of Human monocytic ehrlichiosis were reported in Indiana; six cf these were from southern counties. To test whether Ehrlichia cha ffeensis is present in native ticks, adult ticks representing two species, Dermacentor variabilis and Amblyomma americanum were collected in southern Indiana during the first week of May, 1995 and tested by PCR. A total of 510 D. variabilis ticks were collected and placed into 102 pools (5 ticks per pool). A total of 430 adult A. americanum ticks were collected and placed into 88 pools of 5 ticks or less.D. variabilis ticks were used to optimize the isolation of PCR amplifiable DNA and determine the minimum number of bacterial cells detected. A modified CTAB-phenol method permitted the detection of as few as 100 bacterial cells. The optimal amount of isolated DNA for a PCR amplification was determined to be 2.7 pg of total nucleic acid.The 88 pools of adult A. americanum were subjected to DNA isolation, PCR amplification, and Southern analysis to determine the presence of E. chaffeensis bacteria. Using the 16S rRNA gene from E. chaffeensis, with species specific primers Hut and HE3. Of the 88 pools (430 ticks; 21 were determined to be positive for the presence E. chaffeensis bacteria. Thus, minimal field infection rate for adult A. americanum ticks in Southern Indiana was 4.88%. This calculation is based upon the assumption that at least one tick was positive in each positive pool. / Department of Biology

This thesis is an evaluation of the campaign to pass a Judicial Amendment to the Indiana Constitution. The study first explores constitutional revision throughout the United States and then focuses on Indiana. The study traces the origins of the Amendment as well as its content. Next the organization of the Judicial Amendment campaign is traced, as well as the role of various participating groups in advocacy and education. In addition the thesis statistically explores the success or failure of many campaign activities, including the effect of interest groups and urbanization on voting patterns.

A population of all-female A. tremblayi salamanders from Ginn-Nixon Woods (Delaware County, Indiana) was the subject of this study. These salamanders are members of the Ambystoma jeffersonianum complex which consists of two diploid species (the northern A. laterale and the southern A. jeffersonianum) and two all-female, triploid species (A. tremblayi and A. platineum). The triploid species are believed to be intermediate in range and tolerance to their diploid allies and are usually found associated with them (A. tremblayi with A. laterale and A. platineum with A. jeffersonianum). In this sympatric alliance, the all-female triploids rely on the diploid ales for sperm, which in this case, apparently only activate the eggs without fertilizing them.The population of A. tremblayi in Ginn-NixonWoods is allopatric to all other members of the complex and has evolved a method of reproduction in which no courtship occurs and no spermatophores are picked up. Apparently, however, these A. tremblayi females do require the presence of male A. texanum, a more distantly related ambystomatid, in order to deposit viable eggs.A - maculatum, A. texanum, A. tigrinum and A. tremblayi salamanders were captured in pitfalls along a drift fence as they migrated to the vernal breeding ponds.Pairings of A. tremblayi females with males of the sympatric species in the breeding cages established that the only successful reproduction (through metamorphosis) occurred in the presence of the male A. texanum, although no courtship behavior was observed and no spermatophores were deposited.Cytological studies of the A. tremblayi females in this population, including karyotyping, revealed that the animals are indeed triploid with a chromosome complement of 42 (N = 14). Two of the three chromosomes which were identified as belonging to group three possessed a distinct secondary constriction not previously reported in the literature. It is proposed that this cytological marker identifies these two chromosomes as being derived from A. laterale and lends further support to the hypothesis that A. tremblayi is 'two-thirds A. laterale and one-third A. jeffersonianum' as proposed by Uzzell inhis studies of the Ambystoma jeffersonianum complex.

The purpose of this study was to determine if the type of recommendations made by the school psychologist on the psychological report affected teacher perception of the school psychologist and if the type of recommendations influenced the frequency of utilization of the report. An attempt was made in this study to make the recommendations of the report more useful to special education teachers. Since their training included knowledge of methods and materials to use with children with problems, areas typically covered in the recommendations of a psychological report, these areas were deleted. In this study the recommendations were made which directed the teachers to prewritten goals which could be used in writing a student's individual educational plan (IEP). As a requirement of the Education for Handicapped Children Act, 1975, an IEP with long and short-range goals must be written for each child in special education. An assumption was made that by lessening the teacher's work in writing the IEP, the school psychologist might be perceived as more useful. An end result might be a more positive attitude toward the school psychologist as well as an increased utilization of the report. The pre-written IEP goals used in the study were the 2,400 "identifying behaviors" of the Behavioral Characteristics Progression published by the Vort Corporation. These field-tested behaviors were developmentally sequenced into 59 goal areas.The subjects included all of the Coweta county (Georgia) special education teachers (N = 32) whose students were required to have a psychoeducational evaluation before entrance into those teachers' respective programs. All the teachers were licensed by the state of Georgia as certified or provisionally certified special education teachers.The posttest only control group design was used. The subjects were randomly assigned to groups Table of Random Numbers. Each group, control and experimental, had an N of 16. A six-month duration was set. Both groups received the exact treatment exception of the independent variable, the type of recommendations made in the written psychological report. All the subjects in both groups were asked to mark a tracking sheet which had been attached to each psychological report. The tracking sheet listed eight instances for which a psychological report could be used. The subjects were to mark the appropriate instance each time the report was used. The tracking sheet was developed by the researcher for this study. At the end of six' months the tracking sheets were collected and a semantic differential using 15 pairs of bipolar factor analyzed evaluation-loaded adjectives derived from C. E. Osgood's work was administered to all subjects. The semantic differential was used to measure attitude toward the school psychologist. The tracking sheets were used to determine frequency of utilization of the psychological reports.A t-test for independent samples was performed using the mean scores from the semantic differentials and yielded an insignificant difference (at the .05 level of confidence) in attitude toward the school psychologist as a result of the treatment of recommendations. One dimensional chi square statistical procedures were applied to determine if the frequency of utilization of the reports was significant between the two groups. The frequency of utilization was significantly greater (at the .05 level of confidence) as a result the treatment of the recommendations.Based upon analysis of the data the following conclusions were made:1. No significant difference in attitude toward the school psychologist existed between two groups of special education teachers as a result of the treatment of recommendations in the psychological report.2. As a result of the treatment of the recommendations in the psychological report, the frequency of utilization of the report significantly increased.

In April and May of 1976 an anomalous situation occurred in that no tornadoes were reported for Indiana. This study attempts to determine possible relationships that may have caused the anomalous situation. To better understand the factors surrounding this situation, this study deals with a physical survey of Indiana and a review of literature involving conditions for tornado development. The study tests for relationships between tornadoes, storm tracks, and storm track location relevant to Indiana for the months of March, April, and May, 1955 to 1976, that might suggest reasons for the anomalous situation occurring. Statistical analyses involving correlation tests and analysis of variance, are used to test for possible relationships.

There is no abstract available for this dissertation.

There is no abstract available for this dissertation.

Thesis--University of Chicago. / Photoreproduction of typescript. Includes bibliographical references.

Thesis--University of Chicago. / Photoreproduction of typescript. Includes bibliographical references.

Thesis (Ed. D.)--Indiana University. / Bibliography: p. 43-45.