L'étude systématique des concentrations en tritium et en oxygène- 18 dans les eaux superficielles et souterraines a permis de mettre en évidence certaines modalités des circulations . Les premiers bilans réalisés ont montré, entre autres, que l'incertitude la plus importante était commise dans l'évaluation de l'infiltration. Aussi, le but de cette étude a été de préciser ce paramètre en utilisant les techniques isotopiques depuis l'échelle du mètre (lysimètre), jusqu'à celle du kilomètre (bassin versant).

Lymphocytic infiltration is a feature of basal breast cancer tumors. CXCL10 is a chemokine that was found expressed higher in basal tumor RNA compared to estrogen receptor positive tumor RNA. Both CXCL10 and its receptor CXCR3 were expressed in familial breast cancer tissues, a proportion of which have a basal phenotype. CXCL10 expression was associated with lymphocytic infiltration, and with CXCR3 expression. CXCL10 ligand and receptor were overexpressed individually or together in the human MCF7 cell line. Recombinant human CXCL10 was found to dose dependently decrease cell proliferation. CXCR3 and CXCL10-CXCR3 expressing cells had the potential for increased migration independent of CXCL10 concentration. Co-expression of both genes increased proMMP-2 levels, and conditioned media from one of these clones chemoattracted more of the CXCR3 clones, CXCL10-CXCR3 clones, and CD4+ T-lymphocytes. CXCL10 neutralization suggested that CXCL10 could play a role in this chemoattraction, though it is likely not the only factor involved.

Lymphocytic infiltration is a feature of basal breast cancer tumors. CXCL10 is a chemokine that was found expressed higher in basal tumor RNA compared to estrogen receptor positive tumor RNA. Both CXCL10 and its receptor CXCR3 were expressed in familial breast cancer tissues, a proportion of which have a basal phenotype. CXCL10 expression was associated with lymphocytic infiltration, and with CXCR3 expression. CXCL10 ligand and receptor were overexpressed individually or together in the human MCF7 cell line. Recombinant human CXCL10 was found to dose dependently decrease cell proliferation. CXCR3 and CXCL10-CXCR3 expressing cells had the potential for increased migration independent of CXCL10 concentration. Co-expression of both genes increased proMMP-2 levels, and conditioned media from one of these clones chemoattracted more of the CXCR3 clones, CXCL10-CXCR3 clones, and CD4+ T-lymphocytes. CXCL10 neutralization suggested that CXCL10 could play a role in this chemoattraction, though it is likely not the only factor involved.

Nous poursuivons ici une réflexion sur la ville en tant qu'espace de représentation et source d'inspiration artistique. Notre mémoire-création rassemblera différentes approches d'infiltration artistique afin de mieux en comprendre les composantes. Nous tenterons de définir une esthétique générale de l'infiltration et poserons la question suivante : l'infiltration artistique ne correspondrait-elle pas à une nouvelle forme de dramaturgie urbaine par la fragmentation et l'articulation d'un récit dans l'espace urbain? Cette recherche présente les mutations de certaines pratiques artistiques qui, depuis les quarante dernières années, ont adopté une approche plus sociale. Notre sélection d'exemples présente des artistes qui ont délaissé les lieux de diffusion institutionnels pour aller vers la création in situ et l'espace public. Ces démarches de création cherchent, entre autres, à rendre l'art plus accessible à l'ensemble de la communauté. Nous nous efforçons également d'utiliser l'art comme moyen pour retrouver un lien collectif et donner aux espaces communs des symboles auxquels nous pouvons nous identifier. La création d'Anomalies synthétiques, œuvre en deux temps, vient appuyer cette recherche. Sur une période de dix semaines, nous avons fait vivre une fiction en utilisant différentes formes d'infiltration. Un parcours urbain présenté sur trois soirs a conclu cet essai. Le spectateur a pu découvrir la fiction urbaine sous forme de marche autoguidée par une carte géographique. Ce parcours se voulait une synthèse des dix semaines d'infiltration, soit l'expérience de la représentation transposée à l'espace urbain. ______________________________________________________________________________ MOTS-CLÉS DE L’AUTEUR : infiltration artistique, art interdisciplinaire, théâtre hors les murs, performance, art urbain

Near-surface sediments play an important role in governing the movement of water and contaminants from the land surface through the vadose zone to groundwater. Generally, low permeability surficial soils restrict water flow through the vadose zone and form a natural protective barrier to migration of surface applied contaminants. These types of fine-grained soils commonly contain macropores, such as fractures, animal burrows, and root holes, that have been identified as preferential flow pathways in the subsurface. Accordingly, macropores have the potential to influence groundwater recharge rates and compromise the protective capacity of surficial soils, particularly where the overburden is thin and aquifers are close to the surface. Partially saturated flow and transport in these environments is inherently complex and not well understood. The objective of this thesis was to examine preferential flow processes and the associated movement of contaminants in macroporous, low permeability soils. This was accomplished by conducting numerical and field experiments to investigate and describe the dynamics of macropore flow during episodic infiltration through the vadose zone and evaluate the corresponding influence of macropores on vertical water flow and contaminant transport. Numerical simulations were conducted to identify the important physical factors controlling flow and transport behaviour in partially saturated, fractured soils. A three-dimensional discrete fracture model, HydroGeoSphere, was used to simulate infiltration into homogeneous soil blocks containing a single vertical rough-walled fracture. Relatively large rainfall events with return periods ranging from 5 to 100 years were used, since they are more likely to generate significant preferential flow. Initial results showed that flow system dynamics were considerably more sensitive to matrix properties, namely permeability and antecedent moisture content, than fracture properties. Capillary forces, combined with the larger water storage capacity in the soil matrix, resulted in significant fracture-matrix interaction which effectively limited preferential flow down the fracture. It is also believed that fracture-matrix interaction reduced the influence of fracture roughness and other related small-scale fracture properties. The results imply that aperture variability within individual fractures may be neglected when modeling water flow through unsaturated soils. Nevertheless, fracture flow was still an important process since the fracture carried the majority of the water flow and virtually all of the mass of a surface applied tracer to depth in the soil profile. Model runs designed to assess transport variability under a variety of different physical settings, including a wider range of soil types, were also completed. Vertical contaminant fluxes were examined at several depths in the soil profile. The results showed that the presence of macropores (in the form of fractures) was more important than matrix permeability in controlling the rate of contaminant migration through soils. The depth of contaminant migration was strongly dependent on the antecedent moisture content and the presence of vertically connected fractures. Soil moisture content played a pivotal role in determining the onset and extent of preferential flow, with initially wet soils much more prone to macropore flow and deep contaminant migration. Simulations showed that surface applied tracers were able to reach the base of 2 m thick fractured soil profiles under wetter soil conditions (i.e., shallow water table). Likewise, long-duration, low-intensity rainfall events that caused the soil to wet up more resulted in proportionately more contaminant flux at depth. Fractured soils were particularly susceptible to rapid colloid movement with particle travel times to depths of 2 m on the order of minutes. The main implication is that the vulnerability of shallow groundwater is related more to vertical macropore continuity and moisture conditions in the soil profile, rather than traditional factors such as soil thickness and permeability. Macropore flow and transport processes under field conditions were investigated using small-scale infiltration experiments at sites in Elora and Walkerton, Ontario. A series of equal-volume infiltration experiments were conducted at both sites using a tension infiltrometer (TI) to control the (negative) infiltration pressures and hence the potential for macropore flow. A simulated rainfall experiment was also conducted on a small plot at Walkerton for comparison with the TI tests. Brilliant Blue FCF dye and fluorescent microsphere tracers were applied in all tests as surrogates for dissolved and colloidal contaminant species, respectively. Upon completion of infiltration, excavations were completed to examine and photograph the dye-stained flow patterns, map soil and macropore features, and collect soil samples for analysis of microspheres. Cylindrical macropores, in the form of earthworm burrows, were the most prevalent macropore type at both sites. In the TI tests, there was a clear relationship between the vertical extent of infiltration and the maximum pressure head applied to the TI disc. Larger infiltration pressures resulted in increased infiltration rates, more spatial and temporal variability in soil water content, and increased depths of dye penetration, all of which were attributed to preferential flow along macropores. Preferential flow was limited to tests with applied pressure heads greater than -3 cm. Under the largest applied pressures (greater than -1.0 cm), dye staining was observed between 0.7 and 1.0 m depth, which is near the seasonal maximum water table depth at both field sites. The tension infiltrometer was also used to infiltrate dye along an exposed vertical soil face, thereby providing a rare opportunity to directly observe transient macropore flow processes. The resulting vertical flow velocities within the macropores were on the order of tens of meters per day, illustrating the potential for rapid subsurface flow in macropores, even under partially saturated conditions. The results suggest that significant flow occurred in partially saturated macropores and this was supported by simple calculations using recent liquid configuration models for describing flow in idealized macropores. On all excavated sections, microspheres were preferentially retained (relative to the dye) in the top five centimeters of the soil profile. Below this zone, dye patterns correlated well with the presence of microspheres in the soil samples. There was evidence for increased retention of microspheres at lower water contents as well as a slightly greater extent of transport for smaller microspheres. In general, the microsphere and dye distributions were clearly dictated by vadose zone flow processes. As in the numerical experiments, water storage in the soil matrix and related macropore-matrix interaction were important factors. Mass transfer of water through the macropore walls promoted flow initiation in the macropores near surface. Deeper in the soil, water drawn away from the macropores into the matrix significantly retarded the downward movement of water along the macropores. Imbibition of dye from the macropores into the matrix was repeatedly observed on excavated soil sections and during the transient dye test. Microspheres were also transported laterally into the soil matrix indicating that conceptual models for colloid transport in the vadose zone need to account for this mass transfer process. Overall, the tension infiltrometer performed extremely well as a tool for controlling macropore flow under field conditions and, together with the dye and microsphere tracers, provided unique and valuable insights into small-scale flow and transport behavior. The field experiments raise concerns about the vulnerability of shallow groundwater in regions with thin, macroporous soils. Only a fraction of the visible macropores contributed to flow and transport at depths greater than 40 cm. However, with dye and microsphere transport observed to more than 1.0 m depth, rapid macropore flow velocities, and the sheer number of macropores present, there was clearly potential for significant flow and transport to depth via macropores. Under the right conditions, it is reasonable to speculate that macropores may represent a significant pathway for migration of surface applied contaminants to groundwater over the course of a single rainfall event.

Near-surface sediments play an important role in governing the movement of water and contaminants from the land surface through the vadose zone to groundwater. Generally, low permeability surficial soils restrict water flow through the vadose zone and form a natural protective barrier to migration of surface applied contaminants. These types of fine-grained soils commonly contain macropores, such as fractures, animal burrows, and root holes, that have been identified as preferential flow pathways in the subsurface. Accordingly, macropores have the potential to influence groundwater recharge rates and compromise the protective capacity of surficial soils, particularly where the overburden is thin and aquifers are close to the surface. Partially saturated flow and transport in these environments is inherently complex and not well understood. The objective of this thesis was to examine preferential flow processes and the associated movement of contaminants in macroporous, low permeability soils. This was accomplished by conducting numerical and field experiments to investigate and describe the dynamics of macropore flow during episodic infiltration through the vadose zone and evaluate the corresponding influence of macropores on vertical water flow and contaminant transport. Numerical simulations were conducted to identify the important physical factors controlling flow and transport behaviour in partially saturated, fractured soils. A three-dimensional discrete fracture model, HydroGeoSphere, was used to simulate infiltration into homogeneous soil blocks containing a single vertical rough-walled fracture. Relatively large rainfall events with return periods ranging from 5 to 100 years were used, since they are more likely to generate significant preferential flow. Initial results showed that flow system dynamics were considerably more sensitive to matrix properties, namely permeability and antecedent moisture content, than fracture properties. Capillary forces, combined with the larger water storage capacity in the soil matrix, resulted in significant fracture-matrix interaction which effectively limited preferential flow down the fracture. It is also believed that fracture-matrix interaction reduced the influence of fracture roughness and other related small-scale fracture properties. The results imply that aperture variability within individual fractures may be neglected when modeling water flow through unsaturated soils. Nevertheless, fracture flow was still an important process since the fracture carried the majority of the water flow and virtually all of the mass of a surface applied tracer to depth in the soil profile. Model runs designed to assess transport variability under a variety of different physical settings, including a wider range of soil types, were also completed. Vertical contaminant fluxes were examined at several depths in the soil profile. The results showed that the presence of macropores (in the form of fractures) was more important than matrix permeability in controlling the rate of contaminant migration through soils. The depth of contaminant migration was strongly dependent on the antecedent moisture content and the presence of vertically connected fractures. Soil moisture content played a pivotal role in determining the onset and extent of preferential flow, with initially wet soils much more prone to macropore flow and deep contaminant migration. Simulations showed that surface applied tracers were able to reach the base of 2 m thick fractured soil profiles under wetter soil conditions (i.e., shallow water table). Likewise, long-duration, low-intensity rainfall events that caused the soil to wet up more resulted in proportionately more contaminant flux at depth. Fractured soils were particularly susceptible to rapid colloid movement with particle travel times to depths of 2 m on the order of minutes. The main implication is that the vulnerability of shallow groundwater is related more to vertical macropore continuity and moisture conditions in the soil profile, rather than traditional factors such as soil thickness and permeability. Macropore flow and transport processes under field conditions were investigated using small-scale infiltration experiments at sites in Elora and Walkerton, Ontario. A series of equal-volume infiltration experiments were conducted at both sites using a tension infiltrometer (TI) to control the (negative) infiltration pressures and hence the potential for macropore flow. A simulated rainfall experiment was also conducted on a small plot at Walkerton for comparison with the TI tests. Brilliant Blue FCF dye and fluorescent microsphere tracers were applied in all tests as surrogates for dissolved and colloidal contaminant species, respectively. Upon completion of infiltration, excavations were completed to examine and photograph the dye-stained flow patterns, map soil and macropore features, and collect soil samples for analysis of microspheres. Cylindrical macropores, in the form of earthworm burrows, were the most prevalent macropore type at both sites. In the TI tests, there was a clear relationship between the vertical extent of infiltration and the maximum pressure head applied to the TI disc. Larger infiltration pressures resulted in increased infiltration rates, more spatial and temporal variability in soil water content, and increased depths of dye penetration, all of which were attributed to preferential flow along macropores. Preferential flow was limited to tests with applied pressure heads greater than -3 cm. Under the largest applied pressures (greater than -1.0 cm), dye staining was observed between 0.7 and 1.0 m depth, which is near the seasonal maximum water table depth at both field sites. The tension infiltrometer was also used to infiltrate dye along an exposed vertical soil face, thereby providing a rare opportunity to directly observe transient macropore flow processes. The resulting vertical flow velocities within the macropores were on the order of tens of meters per day, illustrating the potential for rapid subsurface flow in macropores, even under partially saturated conditions. The results suggest that significant flow occurred in partially saturated macropores and this was supported by simple calculations using recent liquid configuration models for describing flow in idealized macropores. On all excavated sections, microspheres were preferentially retained (relative to the dye) in the top five centimeters of the soil profile. Below this zone, dye patterns correlated well with the presence of microspheres in the soil samples. There was evidence for increased retention of microspheres at lower water contents as well as a slightly greater extent of transport for smaller microspheres. In general, the microsphere and dye distributions were clearly dictated by vadose zone flow processes. As in the numerical experiments, water storage in the soil matrix and related macropore-matrix interaction were important factors. Mass transfer of water through the macropore walls promoted flow initiation in the macropores near surface. Deeper in the soil, water drawn away from the macropores into the matrix significantly retarded the downward movement of water along the macropores. Imbibition of dye from the macropores into the matrix was repeatedly observed on excavated soil sections and during the transient dye test. Microspheres were also transported laterally into the soil matrix indicating that conceptual models for colloid transport in the vadose zone need to account for this mass transfer process. Overall, the tension infiltrometer performed extremely well as a tool for controlling macropore flow under field conditions and, together with the dye and microsphere tracers, provided unique and valuable insights into small-scale flow and transport behavior. The field experiments raise concerns about the vulnerability of shallow groundwater in regions with thin, macroporous soils. Only a fraction of the visible macropores contributed to flow and transport at depths greater than 40 cm. However, with dye and microsphere transport observed to more than 1.0 m depth, rapid macropore flow velocities, and the sheer number of macropores present, there was clearly potential for significant flow and transport to depth via macropores. Under the right conditions, it is reasonable to speculate that macropores may represent a significant pathway for migration of surface applied contaminants to groundwater over the course of a single rainfall event.

The shallow subsurface controls the partitioning of available energy between sensible and latent heat of the land surface, and the partitioning of available water among evaporation, infiltration, and runoff. It is a key component of both the hydrometeorological system and the terrestrial water cycle. A critical part of any hydrological or hydrometeorological forecast model is therefore the algorithms used to represent the shallow soil processes, which include infiltration, evaporation, runoff, and interflow. For climate models, coupled algorithms called “Land Surface Schemes” (LSSs) are developed to represent the lower boundary conditions that deal with the land-to-atmosphere energy and moisture fluxes. Similar algorithms are implemented in regional watershed models and day-to-day operational water resources forecasting models. It is the primary objective of this thesis to provide improved methods for simulating coupled land surface processes, which can be used as components of LSSs or within existing operational hydrology models. These new methods address a number of specific issues inadequately handled by current models, including the presence of shallow boundary conditions, heterogeneity in infiltration, and infiltration and interflow coupling processes. The main objective of the proposed research is to provide consistent physically-based approach for simulating near surface soil moisture processes, so as to complete the parameterization of the interflow/infiltration algorithm in a Hydrology-Land-Surface scheme MESH. The work mainly focuses on the investigation and development of more physically-based infiltration and interflow algorithms. The hope is to determine appropriate relationships between internal state variables (specifically bulk soil moisture) and system boundary fluxes, while simultaneously reducing the number of nonphysical or unknown model parameters. Fewer parameters lead to reduced calibration requirements for distributed hydrological models and consequently accelerate the transfer of such models to engineering practice. Multiple approaches were taken to provide improved relationships between infiltration and lateral drainage, fluxes and storage. These algorithms were tested by a specialized Richards' equation for sloping soils and Monte Carlo simulations. These tests demonstrated reasonable accuracy and improved representation for the hydrological processes.

Έγιναν 12 αβαθείς γεωτρήσεις (μέγιστο βάθος 1,5m) και λήφθηκαν δείγματα ανά 10 εκατοστά, στο νότιο τμήμα της λεκάνης Δαμασίου στη Θεσσαλία. Το σύνολο των δειγμάτων (36 δείγματα) αναλύθηκαν στο εργαστήριο και έγινε επί τόπου περιγραφή των πυρήνων των γεωτρήσεων. Έπιπλέον έγινε λεπτομερής χαρτογράφηση των εδαφών σε κλίμακα 1:5000. Ο στόχος της διατριβής ήταν να υπολογισθεί ο ρόλος των εδαφικών τύπων στην κατείσδυση του νερού στο καρστικό σύστημα Αμυγδαλέας - Τυρνάβου. Τα στοιχεία που προέκυψαν από τις αναλύσεις δείχνουν ότι μόνο κατά θέσεις ενισχύεται το καρστικό σύστημα, αφού η περατότητα΄που υπολογίσθηκε ήταν μικρή. Το μεγαλύτερο μέρος του ύψους της βροχής απορρέει προς τον Τιταρήσιο ποταμό, που βρίσκεται πλησίον της λεκάνης στο βόρειο τμήμα αυτής. / This study is based on a series of 12 shallow boreholes at a maximum depth of about 1,5m, within the southermost part of Damasion basin, Thessaly. From the boreholes we collected 36 soil samples for laboratory analysis while the entire borehole were described in the field using Mansel Index and lithology description. in the laboratoty samples were analysed for grain size analysis, moisture, plasticity, liquid limiti and placticity. In addition the study area were mapped in a scale of 1:5000. In this study, we have taken the opportunity to investigate the role of the soil in the infiltration of water to the karst system Amugdalea - Tyrnabou. The results showed that only in same places water contributed in the underground movement of the karst system. The hydraulic contuctivity had poor to medium values.

Designing monitoring networks that can discriminate among competing conceptual models is a key challenge for hydrologists. This issue is examined by considering the impact of network design on the utility of measurements for constraining hydrologic prediction uncertainty. Specifically, a three-staged approach was developed and is presented as a set of modeling case studies. The first case study presents a sensitivity analysis that examines conditions under which the proposed measurement method is likely to detect observations associated with the hydrologic process and properties of interest. This application is focused on the use of geomorphic information to estimate infiltration on arid alluvial fans.The second stage is an assessment of the likely utility of the measurement method to determine whether proposed measurements are likely to be useful for identifying hydraulic properties or hydrologic processes. This objective screening approach could reduce the number of unsuccessful uses of geophysical and other indirect measurement methods. A hypothetical site assessment examines whether the measurement method, temporal gravity change, is likely to detect signals associated with drawdown in an unconfined aquifer that occurs in response to pumping. Also, the utility of these measurements for identifying hydraulic conductivity and specific yield was considered.The third stage, an analysis of optimal network design, compares the projected measurement costs with the expected benefits of constraining hydrologic prediction uncertainty. The final case study presents a network design approach for a feasibility assessment of a proposed artificial recharge site. Predefined sets of proposed measurements of temporal gravity change were considered for various measurement times. An ensemble approach was used to assess the likely impact of measurement error on prediction error and uncertainty for different combinations of measurement sets. The ensemble of prediction errors was translated to probability-weighted performance costs for each measurement set using a cost function. Total cost was calculated as the sum of the performance and measurement costs. The optimal measurement set, defined as the set with the lowest total cost, depends on the prediction of interest, the per measurement cost, the maximum risk-based cost associated with the hydrologic prediction, and the treatment of uncertainty in defining performance costs.

The shallow subsurface controls the partitioning of available energy between sensible and latent heat of the land surface, and the partitioning of available water among evaporation, infiltration, and runoff. It is a key component of both the hydrometeorological system and the terrestrial water cycle. A critical part of any hydrological or hydrometeorological forecast model is therefore the algorithms used to represent the shallow soil processes, which include infiltration, evaporation, runoff, and interflow. For climate models, coupled algorithms called “Land Surface Schemes” (LSSs) are developed to represent the lower boundary conditions that deal with the land-to-atmosphere energy and moisture fluxes. Similar algorithms are implemented in regional watershed models and day-to-day operational water resources forecasting models. It is the primary objective of this thesis to provide improved methods for simulating coupled land surface processes, which can be used as components of LSSs or within existing operational hydrology models. These new methods address a number of specific issues inadequately handled by current models, including the presence of shallow boundary conditions, heterogeneity in infiltration, and infiltration and interflow coupling processes. The main objective of the proposed research is to provide consistent physically-based approach for simulating near surface soil moisture processes, so as to complete the parameterization of the interflow/infiltration algorithm in a Hydrology-Land-Surface scheme MESH. The work mainly focuses on the investigation and development of more physically-based infiltration and interflow algorithms. The hope is to determine appropriate relationships between internal state variables (specifically bulk soil moisture) and system boundary fluxes, while simultaneously reducing the number of nonphysical or unknown model parameters. Fewer parameters lead to reduced calibration requirements for distributed hydrological models and consequently accelerate the transfer of such models to engineering practice. Multiple approaches were taken to provide improved relationships between infiltration and lateral drainage, fluxes and storage. These algorithms were tested by a specialized Richards' equation for sloping soils and Monte Carlo simulations. These tests demonstrated reasonable accuracy and improved representation for the hydrological processes.