This purpose of this quantitative study was to examine health beliefs among Caribbean-born university students regarding acceptance or rejection of influenza vaccination among populations at institutions of higher education. In addition, acculturation was addressed as a factor affecting cultural health beliefs. A survey was completed by 98 students enrolled at Florida International University during the spring 2018 semester. Linear regression was used to analyze whether cultural health beliefs and acculturation were predictive of beliefs about influenza vaccination and beliefs about perceived barriers to influenza vaccination. The study findings showed cultural health beliefs of the students were statistically significant predictors of their beliefs about influenza vaccination and perceived barriers to influenza vaccination. Also, the levels of acculturation were a statistically significant predictor of students' cultural health beliefs and beliefs about perceived barriers to influenza vaccination. After 5+ years of acculturation in the United States, the students surveyed still held cultural beliefs and perceived barriers to influenza vaccination that contributed to their lack of acceptance of the vaccination. The information gained from this study gives credence to the need for designing health interventions and health messages on influenza vaccination that are culture specific for a college-age population if influenza vaccination acceptance is to be promoted.