Abstract: This work summarizes the current knowledge about removing of nitrogen compounds from wastewater using non-traditional biological methods. They include for example process of nitritation/denitritation and a process of deammonification. Special innovative biological methods are economically advantageous because of savings of oxygen for the oxidation of N-ammon and organic substrate for the formation of N2. Achieving of accumulation of nitrites (achieving of shortcut nitrification) due to suppression of nitrite oxidising bacteria (NOB) is the basis of methods mentioned above. Stable shortcut nitrification is influenced by many factors and achieving of this process in a low-strength wastewater is still problematic, so some new procedures how to optimize it are investigated. This was also the main aim of research that was realized at the Department of Agroenvironmental Chemistry and Plant Nutrition of the Czech university of Life Sciences in Prague.

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical sciences Candidate: Bc. Monika Šmídlová Supervisor: RNDr. Eva Novotná, Ph.D. Title of diploma thesis: Effect of selected cytostatics for the treatment of leukemia on the activity of human carbonyl reducing enzymes Key words: reductases, leukemia, cytostatics, inhibition Anthracycline antibiotics, especially daunorubicin, are widely used for the treatment of acute myeloid leukemia (AML) and acute lymphocytic leukemia (ALL). Although the efficacy of these drugs is high, treatment is still limited due to cardiotoxicity and tumor cell resistance to anthracyclines. Mechanisms that contribute to the formation of anthracycline resistance include metabolic biotransformation (reduction) to less efficient secondary alcohols. The reduction is calatyzed by carbonyl reducing enzymes belonging to aldo-keto reductase (AKR) and short chain dehydrogenase/reductase (SDR) superfamilies. The discovery of AKR and SDR inhibitors could help to overcome anthracycline resistance and also reduce cardiotoxicity caused by these drugs. The aim of the diploma thesis was to find out whether all-trans-retinoic acid, cyclophosphamide, cytarabine, cladribine and prednisolone are able to inhibit anthracycline reductases AKR1A1, AKR1B10, AKR1C3, AKR7A2...

The ability of alumino-silicate systems to immobilize hazardous compounds has been investigated since 1990s. The aim of this work is to develope alumino-silicate system (matrix) based on industrial waste products with ability to immobilize hazardous compounds (heavy metals). This ability of the matrix was confirmed by leaching tests based on the law 294/2005 Sb. Concentration of heavy metals in leachates was determined by ICP-MS method. Alumino-silicate system prepared in this work consists of high-temperature fly ash and blast furnace slag activated by mixture of alkaline activators (hydroxide and water glass). Matrixes were characterized by suitable analytic methods (XRD, SEM, FT-IR, DTA-TGA-EGA). The same analytic methods were used to describe the mechanism of immobilization of selected elements (Ba, Cu, Pb) in prepared systems.

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Alžbeta Tomanová Supervisor: RNDr. Eva Novotná, Ph.D. Title of diploma thesis: Effect of selected tyrosine kinase inhibitors on the activity of human carbonyl reducing enzymes Key words: tyrosin kinases, screening, carbonyl, daunorubicin, inhibition Tyrosine kinases are a subclass of protein kinases, catalysing the transfer of ATP phosphate residue to a protein, thereby playing an important role in cellular signaling. Abnormal tyrosine kinase activity is present in various malignancies. In certain cases, inhibition of their function can prevent tumor cell proliferation and eventually induce apoptosis. At the same time, some tyrosine kinase inhibitors have demonstrated the ability to inhibit efflux transporters, which are often involved in the development of resistance to anticancer treatment. In this diploma thesis, the inhibitory effect of imatinib, nilotinib, dasatinib and acalabrutinib (tyrosine kinase inhibitors) has been studied on carbonyl reducing enzymes, whose overexpression by tumor cells may lead to resistance to chemotherapy. In particular, in the case of anthracyclines, the reduction of carbonyl group on C-13 results in not only lower cytotoxic activity, but also increased...

The presence of infections caused by Staphylococcus aureus (S. aureus) and its resistant form - methicillin-resistant S. aureus (MRSA) - are more frequent and treatment with increasing resistance becomes increasingly complicated. Early identification of microbiome in the infectious wounds leads to implementation of the correct treatment and thereby saving the patient from the formation of abscesses, amputation of limbs or even death. Presented thesis entitled Progressive tools for the identification of Staphylococcus aureus is focused on the study of the occurrence of these bacteria in clinical samples, design and construction of new detection methods based on magnetic separation and evaluation of the effects of antibiotics and metal nanoparticles on growth and biofilm formation, gene expression and protein composition in S. aureus and MRSA. The most abundant microorganism in microbiome of infections is S. aureus and in our studies was used as model bacteria for the design of fast, accurate and sensitive detection techniques utilizing indirect detection such as: i) detection of product of biochemical reaction between bacterial enzymes and matrix, ii) detection of the amplified gene using gold nanoparticles as an indicator probe, iii) determination of the detection oligonucleotides after sandwich capture of bacteria between micro and nanoparticles, and iv) a multiplex detection of quantum dots using sandwich capture of specific gene from selected bacteria. Subsequently, an influence effect of beta-lactam antibiotics and their complexes with selenium nanoparticles to non-resistant and resistant form of S. aureus was investigated. The significant inhibition of bacterial growth and biofilm formation as well as changes in gene expression and protein composition were achieved in MRSA by using the complexes of antibiotics and selenium nanoparticles.

The aim of this thesis was to derermine the values of toxic effect of chemical sub-stance chemical on the tested organism Daphnia magna. For testing, these materials were selected: polyaluminiumchloride (PAX 18), its aqueous solution (PAX 19) and potassium dichromate. The thesis is divided into two parts, in the first half I devote to theory of the basic terminology of the issue and the general characterization of the tested organism. In the practical part I focus on the characteristics of the used substances, the course of the tes-ting and the results of the work together with the discussion. Experimental concentrations for PAX 18 were between 1-100 mg.l-1, for PAX 19 10-300 mg.l-1 and for potassium dichromate 40-150 mg.l-1. Testing was perfomed in 3 repeatings with ten individuals + control. 24hEC50 values were: for PAX 18 45,06 mg.l-1, for PAX 19 97,82 mg.l-1 and for potassium dichromate 1,569 mg.l-1. The values of 48hEC50 for PAX 18 byly 22,62 mg.l-1, for PAX 19 37,20 mg.l-1 and for potassium dichromate 0,864 mg.l-1. In all tests, fluctuations in pH and dissolved oxygen content, as well as the number of inhibited individuals, were observed. The results of the observations were plotted and then commented verbally.

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Petra Lakomá Supervisor: RNDr. Eva Novotná, Ph.D. Title of diploma thesis: The effect of alisertib and brigatinib on the activity of selected human carbonyl reducing enzymes Key words: brigatinib, alisertib, daunorubicin, inhibition, carbonyl-reducing enzymes Protein kinases are enzymes, whose main function is based on a transfer of phosphate group from ATP to protein substrate. This common posttranslational modification is involved in the regulation of intracellular processes and cell signaling. Altered expression of protein kinases is often coupled with a development of cancer. Inhibition of protein kinases may prevent cancer cell proliferation and induce their cell death. The main aim of the diploma thesis was to measure inhibition potential of protein kinase inhibitors, alisertib and brigatinib, against carbonyl-reducing enzymes. Overexpression of carbonyl-reducing enzymes in cancer cells may cause resistance to drugs followed by failure of chemotherapeutic therapy. In case of antracyclin chemotherapeutic daunorubicin, carbonyl-reducing enzymes reduce the carbonyl in C-13 giving rise a primary metabolite daunorubicinol, which has lower cytotoxic effect but higher cardiotoxicity. The effort to...

The thesis is focused on the study of allelopathy and allelopathic effects of Ambrosia artemisiifolia and Festuca rubra on selected species of herbs. The first theoretical part describes plants relationships and available information about allelopathy. The second part deals with the influence of Ambrosia artemisiifolia on the growth of Plantago lanceolata, Lotus corniculatus, Arrhenatherum elatius, Festuca rubra, Phacelia tanacetifolia and Pisum sativum in outdoor conditions in different cultivation substrates. The effect of extracts from roots of Festuca rubra and synthetic m-tyrosine on the germination of seeds Ambrosia artemisiifolia was evaluated. The obtained results confirmed the effect of Ambrosia artemisiifolia on the growth of the studied plants and the effect of the tested solutions on germination seeds of Ambrosia.

This thesis deals with the evaluation of toxicity and soil leachate from landfill Těmice. The theoretical part focuses on the characteristics of municipal waste landfill and process description. Another part is devoted to the field of ecotoxicology and describing its basic methods. The practical part describes the selected landfill and performing the assay inhibition of white mustard (Sinapis alba L.) for leachate water from landfill. The work also includes the test phytotoxicity of soil by Phytotoxkit. It focuses on the description of this method, workflow and work aids. In conclusion, the evaluation of both tests, which involve the calculation of root growth inhibition for each concentration of the test substance. The work is complemented by photographic documentation carried out laboratory tests and landfill.

The response inhibition ability is part of executive functions, which may be defined as a set of higher cognitive processes particularly located in the frontal-subcortical circuits. Since the main obsessive-compulsive disorder (OCD) symptoms seem to be relevant to the inability of inhibiting certain stimuli, it can be assumed that response inhibition in these patients will be disrupted. Studies related to this topic have brought inconsistent results. Our research dealing with OCD patients has focused on two dimensions of the response inhibition - the behavioral inhibition, which generally includes the behavior control (for instance impulse control), and the cognitive interference, which is considered to be the cognitive component of inhibition process and is mostly associated with the control of internal cognitive processes. The first part of our research included the verification of hypothesis, stating that the increased severity of obsession is associated with the degree of disrupted ability of cognitive interference, while the severity of compulsions correlates with the degree of disrupted performance in tests measuring behavioral inhibition. The second research objective was to clarify whether the inhibition response ability was different between patients when the predominance of symptoms was...