This diploma thesis (Heterotrophic incorporation of selenium in Chlorella sp.) focuses on characterisation of the genus Chlorella and large-scale use of algae for biofuel and starch production. Besides that, the thesis contains an up-to-date summary of forms of autotrophic algae cultivation ranging from large-volume tanks covering the area of dozens of hectares down to laboratory cultivation in photobioreactors and heterotrophic cultivation of algae in fermenters. Experimental part of the thesis focuses on cultivation and observation of the heterotrophic growth of algae Chlorella vulgaris in relation to the concentration of added selenium which was performed on glucose substrate with varying concentrations and forms of selenium. Selenium was applied in a form of sodium selenite Na2SeO3 and sodium selenate Na2SeO4. Numerous experimental repetitions suggest a significantly important effect of the form of the added selenium on growth rate and vitality of Chlorella sp. While the algae did not thrive on sodium selenite medium, they prospered on sodium selenate and exhibited faster growth compared to that on the media without additional selenium.

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical technology Consultant: PharmDr. Ondřej Holas, Ph.D. Student: Silvia Bacskaiová Title of Thesis: Biodegradable polymeric nanoparticles preparation The present scientific progress in an important rate conduces to nanomedicine development, which aims to reengineering of cancer pharmacotherapy and other substantial diseases. The main intention of this graduation thesis is the study of surface-active chemical's effects on the final properties of nanoparticles. The theoretical part is focused on the nanoparticles likedrugs vehicles, synthesis of biodegradable nanoparticles, themselves applications in the cancer therapy, diseases accompanied by inflammations, vaccination and for another different purposes. The theoretical part also contents characterization of active and passive goal- directed distribution by diseases, microencapsulations, synthesis of nanoparticles by polymerization and from previously synthesized polymers and definition of physical- chemical properties of nanoparticles. The experimental part is more extensive and concerned with the optimalization of reaction conditions of nanoparticles synthesis, with selection of advisable concentracion and type of surfactant. In the experimental part were...

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Consultant: doc. RNDr. Milan Dittrich, CSc. Student: Josef Mašek Title of Thesis: Particulate medicated systems This diploma thesis was focused on experimetal study of influence of technological parameters on the characteristics of nanoparticles made of terpolymer of aliphatic hydroxy acids with tripentaerythritol. In the theoretical part of this thesis nanoparticles as a system for targeted drug distribution were described. Methods of preparation of these nanoparticles, microencapsulation techniques, targeting, basic parameters of the nanoparticles, their size and zeta potential were mentioned in the theoretical part. The experiments were focused on influence of formulation factors, as detergent, concentration of the drug and polymer device on polydispersity of the nanoparticles, their zeta potential and encapsulation effectivity. The influence of detergent formulation on formation and parameters of the nanoparticles was proved. Methylsulfonylmethan was proved for the next experimental procedure as a suitable non-toxic solvent in compositions of biodegradable polymers.

States have traditionally dealt with foreign companies on their territory within their private international law. The arrival of European law and enactment of freedom of establishment though considerably constrained capabilities of member states, whereas companies gained the right of establishment. The aim of this thesis is characterization and specification of the relationship between the freedom of establishment, member states and companies incorporated under laws of member states. The main focus of this thesis is analysis of ten most important cases by the Court of Justice of the European Union concerning the freedom of establishment of companies, which significantly transformed mentioned relationship. Subsequently the thesis summarizes the conclusions of analyzed cases by subject of evolution of primary and secondary freedom of establishment, standing of pseudoforeign companies, conformity with incorporation theory and real seat theory and lastly ways of statutory and/or real seat transfer. The main contribution of the thesis is the very detailed case law analysis, including the newest case Vale from July 12th 2012, and also the case law classification by object.

This diploma thesis deals with hyaluronan modified by palmitoyl and its gelation. Gels were created from palmitoyl hyaluronan with molecular weight 216 kDa and degree of substitution 11 % in concentrations 15 and 20 g dm-3 in water and concentrations 10, 15, 20 g dm-3 in NaCl and TSB. Also gel from palmitoyl hyaluronan with molecular weight 35 kDa and degree of substitution 10 % in concentrations 20, 30 g dm-3 in NaCl was created. Gels were investigated concerning medical applications. Gels were rigid and had very good properties, which was confirmed by rheology. The physical properties (pH, water content) of gels and stability were investigated. On the grounds of the MTT test, three methods of cell incorporation were suggested. Gels are nontoxic, biocompatible, and biodegradable with nontoxic degradation products and that is why they are excellent aspirants for use in biomedicine.

The object of this diploma thesis is the incorporation of Luxembourg among the Lands of the Bohemian Crown. This theme was chosen primarily for the reason that it have not been compiled and analyzed by historiography so far. Available literature usually takes the incorporation of Luxembourg in the Bohemian Crown automatically as a fact without further reasoning. As a result of this approach there is considerable amount of inaccuracies and mistakes in literature. Primary inaccuracy is in a date or in a time period determining the incorporation of Luxembourg in the Bohemian Crown, when literature often indicate the reign of King Charles IV or even the reign of King John of Luxembourg. The purpose of this diploma thesis is not only analyzing the process of incorporation, but also disproves continuing inaccuracies. For this reason the diploma thesis draws and analyzes essential facts primarily from original sources and confront them with available literature. First chapter briefly introduces the institution of the Bohemian Crown for her distinguish from the Kingdom of Bohemia. Further it analyzes the terms "crown" and "incorporation". At last it describes a beginning of the Bohemian Crown and analyzes fundamental documents of this institution. Following second chapter describes the personal union...

CHARLES UNIVERSITY FACULTY OF PHARMACY IN HRADEC KRÁLOVÉ DEPARTMENT OF PHARMACEUTICAL TECHNOLOGY Author: Tereza Pokorná Title of Diploma thesis Terbinafine-loaded biodegradable polymeric systems for topical administration Supervisor: PharmDr. Eva Šnejdrová, Ph.D. In the diploma thesis the released properties of solid dispersions with terbinafine, based on polyesters of D,L-lactic acid and glycol acid, branched with pentaerythritol and tripentaerythritol are studied. The theoretical part deals with solid dispersions, their classification and methods of preparation, methods of drug dissolution testing, properties, effect, use of terbinafine and preparations containing terbinafine. In the experimental part, solid dispersions were prepared by the melting method. The drug was incorporated into the polyester in the form of a hydrochloride or a base in a concentration of 10 % or 20 %. Some systems were plasticized with 30% triethyl citrate. Thin layers were formulated from solid dispersions, and drug dissolution tests were performed on phosphate buffer pH 7.4 at 37 řC. Assay of terbinafine released was performed spectrophotometrically at 223 nm and ultra-high performance liquid chromatography. Based on the results of this diploma thesis, it can be clearly stated that the suitable carrier of terbinafine...

In today's world, where globalisation and internationalization becomes part of our daily life, international entrepreneurship turns into a more actual and important issue. The role of law in this system is to set borders and give directions, which reflect the current state of global society. The aim of the diploma thesis Personal status of legal entities in private international law is both to clarify the actual legal situation of determining the personal status of legal entities and to find out the current grade of global legal integration in these aspects, that either encourages or prevents legal entities from international activities. In order to achieve that, firstly, a theoretical definition of the historically evolved theories for determination of personal status of legal entities is given. Subsequently, their main be- nefits and drawbacks are analysed. The first part is concluded with a definition of the scope of the conflict between the two main theories - incorporation theory and real seat theory. Additionally possible ways, how to solve this conflict together with their potential impact, are sought. Further parts of the thesis deal with such terms as recognition of foreign legal entities and cross-border transfer of seat. These all are essential terms that are bound with the per- sonal status of...

CHARLES UNIVERSITY FACULTY OF PHARMACY IN HRADEC KRÁLOVÉ DEPARTMENT OF PHARMACEUTICAL TECHNOLOGY Author: Vlastimil Barák Title of Diploma thesis The influence of formulation factors on the characteristics of terbinafine loaded nanoparticles Supervisor: PharmDr. Eva Šnejdrová, Ph.D. Consultant: Mgr. Juraj Martiška The diploma thesis is focused on biodegradable polymer nanoparticles loaded by terbinafine based on the copolymer of glycolic and lactic acid branched on polyacrylic acid. The nanoprecipitation method was employed, and the influence of formulation factors on nanoparticle characteristics was studied. The following formulation factors were the concentration of the polymer, the amount of terbinafine, and the concentration of surfactant. Nanoparticles of 120 nm to 300 nm were obtained depending on the preparation conditions. The nanoparticle polydispersity was in all cases from 0.080 to 0.230. The prepared nanoparticles were stable, as evidenced by zeta potential values above 38 mV. A positive zeta potential is desirable for dermal and mucosal adhesion in the topical and ocular application of nanoparticles with terbinafine. The amount of polymer used to form nanoparticles has the greatest effect on particle size. With increasing polyester concentration in the internal phase, the size of the...

The doctoral thesis deals with finding the use of some waste and secondary raw materials in the production of special polymer remediation materials for concrete, which could be applied even in constructions, where extreme mechanical and chemical load is occurred. The aim of this doctoral thesis is experimental examination of the possibility of using selected types of waste, including hazardous waste which represent the highest risk to environment, and secondary raw materials as a substitute for the currently used primary fillers in order to reduce the ecological footprint of the product itself. Some types of secondary raw materials, such as filter fly ash contaminated by flue gas denitrification process, are no longer used as a concrete admixture or partial cement substitution due to unwanted release of toxic ammonia (NH3). Mainly for this reason, the thesis deals with the progressive utilization of such types of secondary raw materials as well as with another currently unused waste into polymeric patching, grouting and anchoring materials while preserving or improving the final properties compared to reference materials using only primary raw materials. The result of this thesis is to find out suitable formulations for efficient preparation of special polymeric remediation materials for concrete containing waste and secondary raw materials as fillers. The partial aim of the thesis and a the scientific contribution is an observation of the developed materials internal structure using a modern device, CT tomography, an influence of the filler type on the long-term durability, and last but not least the observation of the rate of pollutants incorporation, found in hazardous waste, into the polymeric matric with the aid of EDX and FTIR analysis.