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A large number of studies have concentrated on golf swing biomechanics, ranging from planar rigid-link models to 3D kinematic analysis. A promising technique, instantaneous screw axis (ISA) theory, has not been covered in the literature and could provide a better true segment rotation approximation. The objectives of this study are to identify ISA location and orientation, as well as segment angular velocity, of the major body segments involved in the golf swing. For all subjects, it was found that the magnitude of maximum angular velocities increased from the most proximal segment (the pelvis) to the most distal segment (the left arm), in accordance with the summation of speeds principle. Furthermore, most subjects achieved their maximum angular velocities in the desired kinematic sequence, where the first maxima was achieved by the most proximal segment and followed by the more distal segments in the kinematic chain.