Based on the Lyapunov stability theorem, a methodology of designing an nth order adaptive integral variable structure derivative estimator (AIVSDE) is proposed in this thesis. The proposed derivative estimator not only is an improved version of the existing AIVSDE, but also can be used to estimate the nth derivative of a smooth signal which has continuous and bounded derivatives up to n+1. Analysis results show that adjusting some of the parameters can facilitate the derivative estimation of signals with higher frequency noise. The adaptive algorithm is incorporated in the estimation scheme for tracking the unknown upper bounded of the input signal as well as their's derivatives. The stability of the proposed derivative estimator is guaranteed, and the comparison between recently proposed derivative estimator of high-order sliding mode control and AIVSDE is also demonstrated.