The era of economic globalization drove by information technology, but global governing mechanism is still in progress, so regional and inter-regional cooperation are the most welcomed options to shape inter-state relations at present, inter alia, the inter-regional cooperation of European Union and ASEAN. This Research is based on neo-functionalism to analysis the ramification effects of interactions between European Union and ASEAN through the sector of natural resources, economic, trade, investment. and regional development. With the success on functional cooperation, European Union and ASEAN negotiate on the policies which may push the functional cooperation spill-over to political aspects, namely Comprehensive Partnership.