A new tool for kinetic evaluation of kinetic rate constants is enabled by a 3rd generation biosensor. The tool is developed to meet the need of reliably experimental information and communication between pharmaceutical companies and regulatory agencies to increase the productivity and decrease the associated risks. Too obtain the necessary competences and resources for this, a project consisting of Attana AB, AstraZeneca AB, Waters Nordic AB and Karlstad University was established. The main aim of the project is to achieve a comprehension understanding of interactions of different character e.g. fast and slow kinetics. This report concerns a fast interaction system. By analyzing a parathyroid hormone system using standard biosensor assays and single cycle kinetics with Attana Cell™ 200 instruments the fast interaction was characterized. The experimental data was analyzed using standard kinetic evaluation and an adaptive interaction distribution algorithm. The latter tool is developed at Karlstad university in order to describe the heterogeneity of interactions. The idea is to use the heterogeneity information as a decision support in drug development. A sub aim was to investigate the feasibility of the single cycle kinetic assays compared to the standard biosensors assays. The results shows a decrease of experimental time by 70% for homogene interaction and the protocol enables assay without or with less regeneration.

As an orbiter cruising to Jupiter will encounter different plasma environments, variety of spacecraft surface charging is expected. This surface potential can lead to inaccurate and wrong in-situ plasma measurements of on-board sensors, which explain the interest in simulating the charging.In this thesis the spacecraft-plasma interactions for a future mission to Jupiter are modelled with the help of the Spacecraft Plasma Interaction System, taking the case of a Jupiter Ganymede Orbiter (JGO) and a Jupiter Europa Orbiter (JEO) as an archetype for a future mission.It is shown that in solar wind at Earth and Jupiter, spacecraft potentials of about 8 V for the JEO, and 10 V to 11 V for the JGO are expected. Furthermore, at a distance of 15 Jupiter radii from Jupiter, the JGO is expected to charge to an electric potential of 2 V, except in the planetary shadow, where it will charge to a high negative potential of -40 V. Moreover, close to the orbit of Callisto, JGO will charge to 12 V in the sun and to 4.6 V in eclipse, due to a high secondary electron emission yield. / <p>Validerat; 20120115 (anonymous)</p>

The analysis of vehicle-structure interaction systems plays a significant role in the design and maintenance of bridges. In recent years, the assessment of the health of existing bridges and the design of new ones has gained significance, in part due to the progress made in the development of faster moving locomotives, the desire for lighter bridges, and the imposition of performance criteria against rare events such as occurrence of earthquakes and fire. A probabilistic analysis would address these issues, and also assist in determination of reliability and in estimating the remaining life of the structure. In this thesis, we aim to develop tools for the probabilistic analysis techniques of state estimation, parameter identification and global response sensitivity analysis of vehicle-structure interaction systems, which are also applicable to the broader class of structural dynamical systems. The thesis is composed of six chapters and three appendices. The contents of these chapters and the appendices are described in brief in the following paragraphs. In chapter 1, we introduce the problem of probabilistic analysis of vehicle-structure interactions. The introduction is organized in three parts, dealing separately with issues of forward problems, inverse problems, and global response sensitivity analysis. We begin with an overview of the modelling and analysis of vehicle-structure interaction systems, including the application of spatial substructuring and mesh partitioning schemes. Following this, we describe Bayesian techniques for state and parameter estimation for the general class of state-space models of dynamical systems, including the application of the Kalman filter and particle filters for state estimation, MCMC sampling based filters for parameter identification, and the extended Kalman filter, the unscented Kalman filter and the ensemble Kalman filter for the problem of combined state and parameter identification. In this context, we present the Rao-Blackwellization method which leads to variance reduction in particle filtering. Finally, we present the techniques of global response sensitivity analysis, including Sobol’s analysis and distance-based measures of sensitivity indices. We provide an outline and a review of literature on each of these topics. In our review of literature, we identify the difficulties encountered when adopting these tools to problems involving vehicle-structure interaction systems, and corresponding to these issues, we identify some open problems for research. These problems are addressed in chapters 2, 3, 4 and 5. In chapter 2, we study the application of finite element modelling, combined with numerical solutions of governing stochastic differential equations, to analyse instrumented nonlinear moving vehicle-structure systems. The focus of the chapter is on achieving computational efficiency by deploying, within a single modeling framework, three sub structuring schemes with different methodological moorings. The schemes considered include spatial substructuring schemes (involving free-interface coupling methods), a spatial mesh partitioning scheme for governing stochastic differential equations (involving the use of a predictor corrector method with implicit integration schemes for linear regions and explicit schemes for local nonlinear regions), and application of the Rao-Blackwellization scheme (which permits the use of Kalman’s filtering for linear substructures and Monte Carlo filters for nonlinear substructures). The main effort in this work is expended on combining these schemes with provisions for interfacing of the substructures by taking into account the relative motion of the vehicle and the supporting structure. The problem is formulated with reference to an archetypal beam and multi-degrees of freedom moving oscillator with spatially localized nonlinear characteristics. The study takes into account imperfections in mathematical modelling, guide way unevenness, and measurement noise. The numerical results demonstrate notable reduction in computational effort achieved on account of introduction of the substructuring schemes. In chapter 3, we address the issue of identification of system parameters of structural systems using dynamical measurement data. When Markov chain Monte Carlo (MCMC) samplers are used in problems of system parameter identification, one would face computational difficulties in dealing with large amount of measurement data and (or) low levels of measurement noise. Such exigencies are likely to occur in problems of parameter identification in dynamical systems when amount of vibratory measurement data and number of parameters to be identified could be large. In such cases, the posterior probability density function of the system parameters tends to have regions of narrow supports and a finite length MCMC chain is unlikely to cover pertinent regions. In this chapter, strategies are proposed based on modification of measurement equations and subsequent corrections, to alleviate this difficulty. This involves artificial enhancement of measurement noise, assimilation of transformed packets of measurements, and a global iteration strategy to improve the choice of prior models. Illustrative examples include a laboratory study on a beam-moving trolley system. In chapter 4, we consider the combined estimation of the system states and parameters of vehicle-structure interaction systems. To this end, we formulate a framework which uses MCMC sampling for parameter estimation and particle filtering for state estimation. In chapters 2 and 3, we described the computational issues faced when adopting these techniques individually. When used together, we come across both sets of issues, and find the complexity of the estimation problem is greatly increased. In this chapter, we address the computational issues by adopting the sub structuring techniques proposed in chapter 2, and the parameter identification method based on modified measurement models presented in chapter 3. The proposed method is illustrated on a computational study on a beam-moving oscillator system with localized nonlinearities, as well as on a laboratory study on a beam-moving trolley system. In chapter 5, we present global response sensitivity indices for structural dynamical systems with random system parameters excited by multiple random excitations. Two new procedures for evaluating global response sensitivity measures with respect to the excitation components are proposed. The first procedure is valid for stationary response of linear systems under stationary random excitations and is based on the notion of Hellinger’s metric of distance between two power spectral density functions. The second procedure is more generally valid and is based on the l2 norm based distance measure between two probability density functions. Specific cases which admit exact solutions are presented and solution procedures based on Monte Carlo simulations for more general class of problems are outlined. The applicability of the proposed procedures to the case of random system parameters is demonstrated using suitable illustrations. Illustrations include studies on a parametrically excited linear system and a nonlinear random vibration problem involving moving oscillator-beam system that considers excitations due to random support motions and guide-way unevenness. In chapter 6 we summarize the contributions made in chapters 2, 3, 4, and 5, and on the basis of these studies, present a few problems for future research. In addition to these chapters, three appendices are included in this thesis. Appendices A and B correspond to chapter 3. In appendix A, we study the effect on the nature of the posterior probability density functions of large measurement data set and small measurement noise. Appendix B illustrates the MCMC sampling based parameter estimation procedure of chapter 3 using a laboratory study on a bending–torsion coupled, geometrically non-linear building frame under earthquake support motion. In appendix C, we present Ito-Taylor time discretization schemes for stochastic delay differential equations found in chapter 5.