Made available in DSpace on 2014-12-17T15:37:15Z (GMT). No. of bitstreams: 1 AlaneMS_DISSERT1red.pdf: 4031089 bytes, checksum: da896508e54d2cd0138d081ad42b95de (MD5) Previous issue date: 2013-03-27 / Funda??o de Apoio ? Pesquisa do Estado do Rio Grande do Norte / The midline/intralaminar nuclei form a remarkable group of nuclei of the medial and dorsal thalamus. The midline nuclei, in rats, comprises the paratenial nuclei (PT), paraventricular (PV), intermediodorsal (IMD), reuniens (Re) and rhomboid (Rh). The intralaminar nuclei comprises the central medial (CM), paracentral (PC), central lateral (CL) and parafascicular (PF). Such nuclei have dense serotonergic innervation originating from the brainstem, especially from the so-called ascending activation system. These nuclei, in turn, send projections to various cortical and subcortical areas, specifically to limbic areas, which suggests the important role of this neurotransmitter in the limbic circuitry. The aim of this study was to characterize the distribution pattern and morphology of serotonin fibers in the nuclei of the midline and intralaminar thalamic of rocky cavy (Kerodon rupestris), a tipical rodent from brazilizan northeast. To reach this aim we used four rock cavies adults. Following the transcardially perfusion with paraformaldehyde and brain microtomy steps was performed immunohistochemistry for serotonin (5-HT), Nissl technique and subsequent achievement and image analysis to characterize the cytoarchitecture of these nuclei and the serotonergic fibers visualized. An analysis was made of Relative Optical Density (ROD) to semi-quantify the concentration of serotonin fibers in the areas of interest. Thus, we observed a cytoarchitectonic arrangement of these nuclei similar to that found in rats. In case of fibers distribution, those immunoreactive to 5-HT were presented in a higher concentration according as ROD in the midline nuclei relative to intralaminar; Re being the core which has a higher pixel value followed by the PV , Rh, IMD and PT. In intralaminar CL showed higher pixels, followed by nuclei CM, PC and PF. The serotonergic fibers were classified as number of varicosities and axon diameter, therefore find three types of fibers distributed through this nuclear complex: fibers rugous, granular and semi-granular. In PV fibers predominated rugous; in PT fibers predominated granular; IMD, CL and PF fibers were represented by semi-granular and Re, Rh, PC and CM fibers showed granular and semi-granular. Morphological characterization of serotonergic fibers and differences in density between the nuclei may suggest different patterns of synaptic organization of this neurotransmitter beyond confirming his large repertoire functional / O complexo nuclear da linha m?dia/intralaminar forma um not?vel grupo de n?cleos da parte medial e dorsal do t?lamo. Os n?cleos da linha m?dia, em ratos, s?o compreendidos pelos n?cleos paratenial (PT), paraventricular (PV), intermediodorsal (IMD), reuniens (Re) e romb?ide (Rh). J? os intralaminares compreendem os n?cleos central medial (CM), paracentral (PC), central lateral (CL) e parafascicular (PF). Tais n?cleos apresentam densa inerva??o seroton?rgica oriunda do tronco encef?lico, a partir, principalmente, do denominado sistema de ativa??o ascendente. Esses n?cleos, por sua vez, emitem proje??es para diversas ?reas corticais e subcorticais, mais especificamente para ?reas l?mbicas, o que prop?e o importante papel desse neurotransmissor na circuitaria l?mbica. O objetivo desse trabalho foi caracterizar o padr?o de distribui??o e morfologia das fibras de seroton?rgicas nos n?cleos da linha m?dia e intralaminares do t?lamo do moc? (Kerodon rupestris), um roedor t?pico da regi?o Nordeste. Para isso, foram utilizados 4 moc?s adultos jovens. Ap?s etapas de perfus?o e microtomia, foi realizada imunoistoqu?mica para serotonina (5-HT), t?cnica de Nissl e posterior coleta e an?lise de imagens a fim de caracterizar a citoarquitetura desses n?cleos, bem como as fibras de 5-HT neles visualizadas. Foi realizada ainda uma an?lise de Densidade ?ptica Relativa (DOR) para semiquantificar a concentra??o de fibras de 5-HT nas ?reas de interesse. Sendo assim, observamos uma distribui??o citoarquitet?nica desses n?cleos semelhante ao observado em ratos. Em se tratando da distribui??o de fibras, aquelas imunorreativas a 5-HT apresentaram-se em maior concentra??o, conforme a DOR, nos n?cleos da linha m?dia em rela??o aos intralaminares, sendo o Re o n?cleo que apresenta maior valor de pixels, seguido do PV, Rh, IMD e PT. Nos intralaminares o CL apresentou maior valor de pixels, seguido dos n?cleos CM, PC e PF. As fibras seroton?rgicas foram classificadas conforme n?mero de varicosidades, bem como di?metro axonal. Assim, encontramos tr?s tipos de fibras distribu?das atrav?s desse complexo nuclear: fibras rugosas, granulares e semi-granulares. No PV predominaram fibras rugosas; PT predominaram fibras granulares; IMD, CL e PF foram representados por fibras semi-granulares e Re, Rh, PC e CM apresentaram fibras granulares e semi-granulares. A caracteriza??o morfol?gica das fibras seroton?rgicas encontradas e as diferen?as de densidades entre os n?cleos permite sugerir diferentes padr?es de organiza??o sin?ptica deste neurotransmissor al?m de confirmar seu grande repert?rio funcional