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Characterization of human glutathione-dependent microsomal prostaglandin E synthase-1 /Thorén, Staffan, January 2003 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2003. / Härtill 5 uppsatser.
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mPGES-1 : a key regulator of fever and neonatal respiratory depression /Saha, Sipra, January 2006 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2006. / Härtill 4 uppsatser.
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mPGES-1 and the PGE₂ pathway in arthritis /Westman, Marie, January 2005 (has links)
Diss. (sammanfattning) Stockholm : Karolinska institutet, 2005. / Härtill 4 uppsatser.
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Overproduction of xylose isomerase in recombinant Escherichia coliSpencer, C. Thomas 11 May 2006 (has links)
Aspects of recombinant enzyme production were characterized in genetically engineered Escherichia coli. Five strains transformed with the xylose isomerase overproduction system (pRK248/pTXI-1) were compared, based on parameters of cell metabolism and inducible enzyme activity in shake flask cultures. E. coli strain LE392 (pRK248/pTXI-l) performed the best with respect to nearly all of the parameters tested. The stability of the overproducing plasmids was tested in prolonged serial shake flask cultures. Segregational instability occurred in cultures lacking antibiotic selective pressure. The onset of this instability was influenced by host strain, plasmid construct and cultivation media. Despite its occurrence, the degree of plasmid instability, even in the worst case, would not be detrimental in the proposed application of this overproduction system. The cell mass and enzyme production of strain LE392 (pRK248/pTXI-1) was characterized in four batch cultures. It was determined that both carbon source starvation and the accumulation of acetate as a byproduct of glucose metabolism diminished the E. coli's ability to produce xylose isomerase. The peak enzyme production was determined to be 1320 International Units (IU) of xylose isomerase activity per liter of culture. The cell mass and enzyme production of strain LE392 (pRK248/pTXI-l) was studied in fed-batch cultures. The amount of xylose isomerase the cells produced was dependent on the degree of glucose limitation they experienced during cultivation. The peak enzyme production was 3250 IU/L. The results of these experiments were interpreted in the context of this overproduction system and its proposed application for the production of ethanol from plant biomass. / Ph. D.
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Quantitative analysis of melanoma transcripts : with emphasis on methodological and biological variation /Farnebäck, Malin, January 2004 (has links) (PDF)
Diss. Linköping : Univ., 2004.
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Prostaglandin E₂ in brain-mediated illness responses /Elander, Louise, January 2010 (has links)
Diss. (sammanfattning) Linköping : Linköpings universitet, 2010. / Härtill 5 uppsatser.
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Inflammation-associated genes and genetic variations in colorectal cancer /Elander, Nils, January 2009 (has links) (PDF)
Diss. (sammanfattning) Linköping : Linköpings universitet, 2009. / Härtill 5 uppsatser.
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