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THE EFFECT OF TEMPERATURE ON THE EXPRESSION OF ENZYMES IN THE VESTIGIAL MUTANT OF DROSOPHILA MELANOGASTERPardy, Rosevelt L. January 1969 (has links)
No description available.
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Activation and function of protein kinase C [eta] in T cells /Resnick, Moira Stephanie. January 1998 (has links)
Thesis (Ph. D.)--University of Virginia, 1998. / Includes bibliographical references (139-184). Also available online through Digital Dissertations.
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Isolation and characterization of two Saccharomyces cerevisiae AICAR transformylase/IMP cyclohydrolase isozymes /Tibbetts, Anne Staker, January 1999 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 1999. / Vita. Includes bibliographical references (leaves 135-144). Available also in a digital version from Dissertation Abstracts.
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Analytical and clinical considerations of creatine kinase isoenzyme-1 /Abbott, Lenox Berchael January 1984 (has links)
No description available.
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Expression and glycosylation of meprin isoformsGorbea, Carlos M. 28 July 2008 (has links)
Meprin A and meprin B are disulfide-linked, oligomeric metalloendopeptidases in renal brush border membranes. Meprin A contains 90-kDa subunits (α subunits) and is expressed in random-bred and some inbred strains of mice. Meprin B contains subunits of 110 kDa (β subunits) <i>in situ</i>, and the enzyme from C3H/He mice, a strain that does not express α subunits, has been characterized. Evidence from this and previous studies indicate that β subunits are expressed in all mouse strains. Meprins were characterized with regard to their glycosylation by lectin blotting. Both meprin A and meprin B bound the lectins concanavalin A and the erythroagglutinin from <i>Phaseolus vulgaris</i> indicating that both enzymes contain high mannose and bisected biantennary complex type oligosaccharides. However, meprin A, but not meprin B, bound the agglutinins from <i>Ricinus communis</i>, <i>Datura stramonium</i>, and the leukoagglutinin from <i>Phaseolus vulgaris</i>, indicating that complex-type N-glycosylation differs in these proteinases. Lectin blots of membrane proteins from C57BL/6 mice indicated that there were differences between adult male and female mice in the glycosylation (specifically in the complex type oligosaccharides) of the α subunit of meprin A. A marked degree of carbohydrate heterogeneity was observed in meprin A from males as compared to the enzyme isolated from female mice. Additionally, the data indicated that at least three of the ten potential glycosylation sites in the meprin α subunit are glycosylated. Overall, these studies expand our understanding of how estrogens affect glycosylation of meprin A. The oligomeric organization of the meprins was examined in brush border membrane fractions from a random-bred strain (lCR) and two inbred strains of mice (C57BL/6 and C3H/He). The random-bred strain contained three oligomeric complexes of approximately 390, 440, and 490 kDa as determined after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-P AGE) in the absence of reducing agents. The subuβnits in all three oligomers were linked by disulfide bridges. Western blotting using anti-α monoclonal antibodies revealed that α subunits (90 kDa) were present in the 390- and 440-kDa complexes. Western blotting with polyclonal antibodies specific for the β subunit (110 kDa) revealed the presence of these subunits in the 440- and 490-kDa complexes. Electroelution of the individual oligomers followed by SDS-PAGE under reducing conditions confirmed that the 390- and 490-kDa molecules are homotetramers of α and β subunits, respectively, and that the 440-kDa complex is a heterotetramer composed of disulfide-linked α and subunits. C57BL/6 mice expressed both α and β subunits and contained tetramers composed of α₄ and α₂β₂. C3H1He mice expressed only the 110-kDa β subunits and the β₄ oligomer. This type of multimeric organization of covalently-linked subunits is unique for the known endopeptidases. Initial cloning of the mouse meprin β subunit revealed that the enzyme belongs to the recently described astacin family of metalloendopeptidases. The β subunit polypeptide had a molecular mass of 88 kDa as determined after SDSPAGE of brush border membrane proteins treated with glycosidases. Nucleotide sequencing, internal peptide sequences from the β subunit, and NH₂-terminal sequence analyses (39 residues) indicated that at the amino acid sequence level, mouse β is approximately 55 % identical to mouse , and 85 % identical to the rat β subunit. These and other studies indicate that α and β are closely related products of divergent evolution. Northern blot analyses of different tissues from C57BL/6 and C3H/He mice indicate that β subunit mRNA can be detected in kidney and intestine, in contrast to the α subunit which is only present in kidney tissue. Initial studies in mouse intestinal brush border membranes indicated that the characteristic latency of kidney β subunits may be absent in the intestinal enzyme. This observation may reflect activation of the mouse β subunit by trypsin in the intestinallumen. The activation of β in the kidney by trypsin-like proteinases is reminiscent of the activation of protein zymogens and may serve as a means of regulation of the proteolytic activity of the proteinase at the cell surface. / Ph. D.
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Dissociation and reassociation of human liver class I alcohol dehydrogenase.January 1993 (has links)
by Ho Ka-Pong, Bosco. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 81-100). / Chapter CHAPTER 1: --- INTRODUCTION --- p.1 / Chapter CHAPTER 2: --- PURIFICATION OF HUMAN CLASS I LIVER ADH --- p.19 / Chapter CHAPTER 3: --- DISSOCIATION AND REASSOCIATION OF HUMAN CLASS I ADH BY FREEZE/THAW TECHNIQUE --- p.36 / Chapter CHAPTER 4: --- "DISSOCIATION AND REASSOCIATION OF HUMAN CLASS I ADH BY USING UREA, GdmCl,HIGH SALT AND LOW pH" --- p.51 / Chapter CHAPTER 5: --- GENERAL DISCUSSION --- p.77 / REFERENCES --- p.81
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Studies on the human liver alcohol dehydrogenase isozymes: genetic variation, purification and characterization.January 1987 (has links)
by Fong Wing-ping. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1987. / Bibliography: leaves 186-198.
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Enzymatic browning of straw mushroom, Volvariella volvacea.January 1999 (has links)
by Suen Tsang. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 96-103). / Abstract also in Chinese. / Chapter Chapter 1: --- Literature review --- p.1 / Chapter 1.1 --- "Straw mushroom, Volvariella volvacea" --- p.1 / Chapter 1.2 --- Problems which restrict the market of straw mushroom --- p.3 / Chapter 1.3 --- Non-enzymatic browning --- p.5 / Chapter 1.4 --- Enzymatic browning --- p.7 / Chapter 1.5 --- Impact of browning --- p.12 / Chapter 1.6 --- Mechanism of inhibition of PPO --- p.13 / Chapter 1.7 --- Sulfites --- p.13 / Chapter 1.8 --- Classification of PPO inhibitors based on chemical property --- p.14 / Chapter 1.9 --- Classification of PPO inhibitors based on inhibitory mechanism --- p.17 / Chapter 1.10 --- Physical methods for prolonging shelf-life --- p.18 / Chapter 1.11 --- Significance of this research --- p.20 / Chapter Chapter2: --- Characterization of PPO in straw mushroom --- p.21 / Chapter 2.1 --- Introduction --- p.21 / Chapter 2.2 --- Materials and Methods --- p.22 / Chapter 2.2.1 --- PPO content in straw mushroom compared to other food sources: potato and pear --- p.22 / Chapter 2.2.2 --- "Optimal pH, enzyme kinetics and localization of PPO in straw mushroom" --- p.24 / Chapter 2.2.3 --- PPO isoenzymes in straw mushroom --- p.25 / Chapter 2.3 --- Results --- p.29 / Chapter 2.3.1 --- PPO content in straw mushroom compared to other food sources: potato and pear --- p.29 / Chapter 2.3.2 --- "Optimal pH, enzyme kinetics and localization of PPO in straw mushroom" --- p.29 / Chapter 2.3.3 --- PPO isoenzymes in straw mushroom --- p.32 / Chapter 2.4 --- Discussion --- p.43 / Chapter Chapter3: --- Several attempts to solve browning problem of straw mushroom --- p.55 / Chapter 3.1 --- Inhibitors of PPO in straw mushroom --- p.55 / Chapter 3.1.1 --- Investigation of inhibitors of PPO in straw mushroom --- p.55 / Chapter 3.1.1.1 --- Materials and methods --- p.55 / Chapter 3.1.1.2 --- Results --- p.56 / Chapter 3.1.2 --- The potential of using a combination of different PPO inhibitors --- p.58 / Chapter 3.1.2.1 --- Materials and methods --- p.58 / Chapter 3.1.2.2 --- Results --- p.59 / Chapter 3.1.3 --- Direct application of PPO inhibitors --- p.61 / Chapter 3.1.3.1 --- Materials and methods --- p.61 / Chapter 3.1.3.2 --- Results --- p.62 / Chapter 3.1.4 --- PPO and lipase content in straw mushroom under post harvest storage --- p.62 / Chapter 3.1.4.1 --- Materials and Methods --- p.74 / Chapter 3.1.4.2 --- Results --- p.75 / Chapter 3.2 --- Vacuum packaging --- p.75 / Chapter 3.2.1 --- Materials and methods --- p.75 / Chapter 3.2.2 --- Results --- p.78 / Chapter 3.4 --- Discussion --- p.78 / Chapter Chapter 4: --- Future work --- p.87 / Chapter 4.1 --- Suggested improvements of experiments --- p.87 / Chapter 4.2 --- Suggested experiment in future: application of calcium chloride --- p.88 / Chapter Chapter 5: --- Conclusion --- p.94 / References --- p.96
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Épidémiologie des leishmanioses dans la région de Marrakech, Maroc effet de l'urbanisation sur la répartition spatio-temporelle des phlébotomes et caractérisation moléculaire de leurs populations /Boussaa, Samia Pesson, Bernard. Boumezzough, Ali. January 2008 (has links) (PDF)
Thèse de doctorat : Écologie, épidémiologie : Strasbourg 1 : 2008. / Titre provenant de l'écran-titre. Bibliogr. p. 200-217.
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Physical status of mitochondrial aspartate aminotransferase in serum and the role of alpha 2-macroglobulin in its clearancePapineni, Venkat Lakshman Rao. January 1993 (has links)
published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy
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