This diploma thesis focuses on an analysis of the sources of pollution in the catchment area of the water source in Skalice nad Svitavou, which is used as a supply of drinking water. The theoretical part of the thesis describes the issues related to the given topic, as well as the area of interest and the water source. Field research was conducted in the catchment area of the water source, and samplings spots for monitoring water quality in the watercourse of Úmoří were determined, as the watercourse runs near the water source. Samples were collected every three months and, subsequently, analysed in the laboratory of ÚAKE AF MENDELU. The findings were evaluated according to the valid legislation. The water quality of the water source was also evaluated for the time period of the last five years. On the basis of all of these findings, protective measures were proposed to improve the protection of the water source.