This thesis deals with low level DC voltage measurement by three methods. First part of this thesis describes each method. It is about potentiometric method, reference step method and direct method. This thesis also describes Josephson voltage standard which was used for calibration nanovoltmeter and Zener reference. The theoretical part of this thesis also deals with the evaluation of key comparison data. The main goal of this thesis is the comparison of methods used to measure low level DC voltage. The practical part of the thesis deals with the implementation of measurement with all methods in cooperation with Czech metrology institute. The measured values are processed including uncertainty evaluations. The final part of this thesis focuses on comparison of measurement methods. The key comparison reference value and the degree of equivalence of the measurement of each method are determined. Next part of comparison consisted of graphic comparison of methods. The end of the thesis contains evaluation of the achieved results.