Please read the abstract in the section 00front of this document / Dissertation (MSc (Zoology))--University of Pretoria, 2007. / Zoology and Entomology / MSc / unrestricted

The “vulnerable” listing by IUCN of cheetah Acinonyx jubatus in Africa has caused urgency in the protection of their habitat and development of predator management strategies. By understanding the movement and home range of cheetah in Botswana, translocation of problem cheetah or reintroduction of non-problem animals can be managed appropriately. More importantly this information will help to protect what is already there. Due to the increasing numbers of cheetahs being taken by illegal trade and poaching, there have become incidents of orphaned cubs where the only option for their survival is rehabilitation for release into the wild, or euthanasia. The first part of the study focuses on baseline information of movement patterns of cheetah in Botswana. Eleven cheetahs were collared and monitored from 2003-2007, including males and females with and without cubs from the Ghanzi and Southern districts. The understanding of cheetah movement is critical in determining methods of protection and survival of the species in protected areas living with competing predators such as lion Panthera leo, brown hyaena Hyaena brunnea and leopard Panthera pardus, as well as on farmlands where human conflict and habitat loss are the main causes of cheetah death. In the Southern district the cheetah were able to move freely in and out of the Jwana Game Reserve surrounded by communal livestock farms with low to medium conflict, utilizing various livestock protection methods, whilst Ghanzi consisted of livestock and game farms where conflict was high and protection methods were limited or nonexistent. Home ranges in males ranged from 492 km2(in single males) to 849 km2(in one coalition) in Ghanzi, while females ranged from 241 km2 to 306 km2 in Jwaneng. In addition, in order to determine the correlation between spoor density and true density, a 15 month spoor study was conducted in Jwaneng at the Jwana Game Reserve on a population of free ranging wild cheetah. A correction factor was tested and adjusted for accuracy, resulting in two formulas to be used in the wet and dry seasons. Spoor surveys are by no means a determinant factor, as they need to be repeated over time to observe population fluctuations due to outside factors, and are time consuming and can be expensive, but they are a management tool that can be utilized for estimations of cheetah densities on private farms or protected areas. The third part to the study was the rehabilitation of three orphaned cubs, from different families, that were put together from eight to twelve weeks old. The goal of this project was to raise and release fully functional, self sufficient, breeding animals into the wild population on a game farm. These cubs were raised in isolation until 1.5 years of age, then transferred to a 100 ha enclosure where they were given the opportunity to learn to hunt. Daily observations of their behavioural development and hunting abilities were recorded for 48 days and are presented in a descriptive way. At two years old they were released onto a 9000 ha game farm where their potential to survive on farmland was monitored. Botswana does not have the facilities or desire to keep predators captive, and if orphaned cubs could be utilized by placing them back into wild populations where they could add to the gene pool, alternatives would be available for captive bred animals or cheetahs facing life long captivity. Copyright / Dissertation (MSc)--University of Pretoria, 2009. / Centre for Wildlife Management / unrestricted

The project was undertaken to establish the normal intestinal flora of healthy cheetahs and to produce a species-specific probiotic for use in juvenile cheetahs in captivity to improve weight gain and reduce diarrhoea. The normal intestinal flora of healthy cheetahs was established using non-selective and selective media. High numbers of anaerobic bacteria and aerobic bacteria were isolated from the faeces of cheetahs in this study. Eight percent of isolates were Enterococcus spp. Both Enterococcus faecium and Lactobacillus Group 1 were selected for use in the probiotic. Twenty-seven juvenile cheetahs between eight and thirteen months of age were included in the probiotic trial (Median: 12 months). The probiotic was fed for 28 days to the Probiotic Group. Both the Probiotic and Control groups were monitored for 70 days prior to the administration of the probiotic and 14 days after administration. The feeding of the cheetah-specific probiotic resulted in an increase of weight in the treatment group (p=0.026, ANOVA, p<0.05) in comparison to the Control Group. There was a relative improvement in the faecal quality in the Probiotic Group in comparison to the Control Group. This was accompanied by an absence of blood and mucus in the faeces, which had been present prior to the start of the 28-day administration of the probiotic. The feeding of a cheetah-specific probiotic resulted in an improved weight gain and food conversion in the Probiotic Group in comparison to the Control Group as well as in a reduction of diarrhoea in the Probiotic Group. More research is needed on the effect of the probiotic on different age groups and animals suffering from specific diseases such as liver disease and gastritis. / Dissertation (MSc (Veterinary Science))--University of Pretoria, 2004. / Veterinary Tropical Diseases / unrestricted

Cheetah (Acinonyx jubatus) persist under continued conservation threat in small populations mostly in protected areas in an historically reduced geographic range. Home range, a useful trait for threat assessment, species reintroduction, and population estimation, is plastic in cheetah with sizes ranging from 40 km2 to over 1000 km2 depending on location. Previous home range estimates for cheetah used the minimum convex polygon (MCP), assuming asymptotic home ranges and MCP insensitivity to sample size. They reported metrics of home range size and overlap based on only outline methods. I use 6 481 observations of 240 female and 315 male cheetah from > 60 matrilines over 25 years in the Serengeti Plains to investigate lifetime, core, yearly, and seasonal range size with kernel density estimation. I investigate autocorrelation using time to statistical independence of locations. I confront the assumption of asymptotic home ranges by testing the traditional and multiscaled home range predictions and provide a novel method for determining kernel asymptotes. I challenge the notion of Serengeti cheetah as a migratory carnivore with analyses of site fidelity and objectively defined core ranges. I assess year to year and seasonal location shifts, showing that yearly shifting lessens as females age. I provide quantitative evidence for philopatry in female- and juvenile dispersal in male cheetah of the Serengeti Plains. I use simple overlap metrics to show that overlap in lifetime and core ranges is greater in related than unrelated female pairs. I use multi-response permutation procedures (MRPP) to show that overlap in unrelated female pairs varies with season. I use correlation of utilization distributions to show that avoidance is apparent only in unrelated pairs of females. My results call into question previous MCP estimates of cheetah home range size, and provide guidance for future sampling of cheetah locations. My home range results will guide management of this imperiled species and my methodological findings may be general and applicable to a wide range of taxa. / Master of Science

Sequence variation of a 238 bp segment of the mitochondrial control region was analyzed for 1,568 Steller sea lions (2.8% of the estimated species population) sampled from 50 rookeries representing nearly every locality at which Steller sea lions are known to breed in significant numbers. Haplotype diversity (H = 0.9164 ± 0.0035) was high and nucleotide diversity (π = 0.00967 ± 0.00586) was moderate. No evidence was observed for significant genetic bottleneck effects. Rookeries were grouped into regions and stocks to examine structure at different spatial scales. F- and Φ-statistics were computed for all pairwise comparisons of rookeries, regions and stocks. Significant (P<0.05) divergence of eastern stock (southeastern Alaska to California) animals from western stock animals was supported in analyses at all spatial scales. Likewise, rookeries and regions from Asia were found to be significantly different from all other western stock rookeries. This was most clearly demonstrated using Φ-statistics at the regional level. The Commander Islands clearly associate with Alaskan western stock rookeries, not with the Asian rookeries. Within each of the three stocks there is significant isolation by distance among rookeries. This relationship does not hold for inter-stock comparisons indicating that there are important barriers to gene flow among stocks. Mitochondrial DNA analysis supports the recognition of three stocks for appropriate conservation of the species. The currently recognized eastern stock is unaffected, but the western stock is now partitioned west of the Commander Islands yielding a western stock which ranges from Prince William Sound west to the Commander Islands, and an Asian stock including rookeries from the Kamchatka Peninsula, Kuril Islands, and Sea of Okhtosk.

Sequence variation was examined for the Zinc-finger Y (zfy) gene and the mitochondrial control region for two species of pinnipeds, the Steller sea lion (Eumetopias jubatus) and harbor seal (Phoca vitulina). The two species differ in aspects of their breeding strategies, dispersal, and life histories. Comparable stock sample sizes of males from each species were taken from localities that span at least one wellrecognized phylogeographic stock as defined by mtDNA markers. Variation in zfy, a strictly paternally inherited marker located on the Y chromosome, was low in both species. An interesting pattern of subdivision was found for zfy in harbor seals that was concordant with population subdivision for mtDNA. In Steller sea lions, no such concordant pattern was evident with only a single rare zfy variant being observed. One explanation for the different patterns observed is that dispersal is less in male harbor seals than in male Steller sea lions.

Sequence variation of a 238 bp segment of the mitochondrial control region was analyzed for 1,568 Steller sea lions (2.8% of the estimated species population) sampled from 50 rookeries representing nearly every locality at which Steller sea lions are known to breed in significant numbers. Haplotype diversity (H = 0.9164 ± 0.0035) was high and nucleotide diversity (π = 0.00967 ± 0.00586) was moderate. No evidence was observed for significant genetic bottleneck effects. Rookeries were grouped into regions and stocks to examine structure at different spatial scales. F- and Φ-statistics were computed for all pairwise comparisons of rookeries, regions and stocks. Significant (P<0.05) divergence of eastern stock (southeastern Alaska to California) animals from western stock animals was supported in analyses at all spatial scales. Likewise, rookeries and regions from Asia were found to be significantly different from all other western stock rookeries. This was most clearly demonstrated using Φ-statistics at the regional level. The Commander Islands clearly associate with Alaskan western stock rookeries, not with the Asian rookeries. Within each of the three stocks there is significant isolation by distance among rookeries. This relationship does not hold for inter-stock comparisons indicating that there are important barriers to gene flow among stocks. Mitochondrial DNA analysis supports the recognition of three stocks for appropriate conservation of the species. The currently recognized eastern stock is unaffected, but the western stock is now partitioned west of the Commander Islands yielding a western stock which ranges from Prince William Sound west to the Commander Islands, and an Asian stock including rookeries from the Kamchatka Peninsula, Kuril Islands, and Sea of Okhtosk.

AN ABSTRACT OF THE THESIS OF REGINA HELEN MOSSOTTI, for the Master of Science degree in ZOOLOGY, presented 23 October 2009, at Southern Illinois University Carbondale. TITLE: FEMALE REACTION TO MALE URINE SCENTS AS POTENTIAL INDICATOR OF MATE CHOICE IN CAPTIVE CHEETAHS (ACINONYX JUBATUS) MAJOR PROFESSOR: GEORGE A. FELDHAMER Genetic variation in the cheetah (Acinonyx jubatus) has become dangerously low because of dramatic population decline and fragmentation. Zoos throughout the world manage captive cheetahs with breeding programs to maximize genetic heterozygosity. Unfortunately, the zoo community has not accomplished consistent breeding success with cheetahs, possibly because of a general lack of information on sociosexual behavior. Currently, individual cheetahs are assigned mates based primarily on genetic relatedness; however, evidence from many species suggests that allowing animals to choose mates increases breeding success. When animals, primarily females, are allowed to choose mates they will often pick the best genetic match. I tested whether female cheetahs can determine their genetic relatedness to males by investigating their urine scents. Voided male urine was collected following scent marking. The female was offered scents from three different males: one from an unrelated male, a "good" mate choice (A), another from a male that was equivalent to a second cousin, an "average" mate choice (B), and one from a male that was closely related, equivalent to a brother/father, a "poor" mate choice (C). Every female was also offered "blank" gauze as a control (D). The scents were offered in a pair-wise forced choice paradigm for a total of six possible combinations. All behaviors displayed toward each scent (and their duration) were recorded. The estrous cycling of each female was monitored through fecal hormone evaluation for approximately six weeks, including the weeks during scent trials. In every pairing except C vs. D, the females spent more time overall with the better mate choice in the pair; with three of the comparisons being significantly different (A>C, t=2.38, df=11, P=0.039; A>D, t=1.88, df=11, P= 0.087 and B>D, t=2.62, df=8, P=0.045). Proximity was the most frequently observed behavior and females spent more time in proximity to the most distantly related male scent in all pairings. They spent significantly more time in proximity with A in AC pairing (t=2.25, df=10, P=0.049) and with B in the BC and BD pairings (t=6.37, df=8, P=0.0002; t=2.46, df=6, P=0.049; respectively). Sniffing was the next most frequently observed behavior, and in all pairings (except CD) females spent more time sniffing the most distantly related male's scent; but was only significantly different for A in the AD pairing (t=2.31, df=7, P=0.055). Lag time of estradiol in fecal samples varies between individuals; therefore, the affect of daily estradiol concentrations on scent choices could not be determined. This is the first mate choice study using urine with a mammalian carnivore.

This work is focused on the breeding of cheetahs Acinonyx jubatus bred in captivity. The first part is divided into two main sections and is processed using the available scientific literature. The first section describes the history and current status of taxonomic classification of individual species and subspecies of the genus Acinonyx. The second section deals with general biology of the species - characteristics, habitat, food in the wild, extending the route of exposure, morphology of the reproductive system. Separate chapters are devoted to the problems of rearing animals in breeding facilities - food cheetahs areas for breeding, a special section discusses the issues of reproduction in captivity. In the practical part, an analysis of data from the International studbook Acinonyx jubatus 2013, focusing on the reproductive success of males and females according to their origin and age. Data was transferred to MS Excel and main observed characteristics presented in the form of graphs. The resulting data were statistically analyzed.

Please read the abstract in the section 00front of this document / Dissertation (MSc (Wildlife Management))--University of Pretoria, 2007. / Zoology and Entomology / MSc / Unrestricted