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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

A??o do regulador de crescimento de Artr?podes, Piriproxifen sobre Rhipicephalus sanguineus (Acari: Ixodidae), em infesta??es artificiais, em coelhos. / Action of arthropod growth regulator, pyriproxyfen, on Rhipicephalus sanguineus (Latreille, 1806) in artificial infestations in rabbits.

Ribeiro, Francisco de Assis 09 December 2010 (has links)
Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2018-11-28T12:09:02Z No. of bitstreams: 1 2010 - Francisco de Assis Ribeiro.pdf: 1446521 bytes, checksum: 1539b67c73688bab6449f2b66eab899e (MD5) / Made available in DSpace on 2018-11-28T12:09:03Z (GMT). No. of bitstreams: 1 2010 - Francisco de Assis Ribeiro.pdf: 1446521 bytes, checksum: 1539b67c73688bab6449f2b66eab899e (MD5) Previous issue date: 2010-12-09 / This study aimed to evaluate the proposed amendments in different stages of Rhipicephalus sanguineus submitted to arthropod growth regulator (AGR's), pyriproxyfen, in rabbits treated. The study was conducted on the premises of the Laborat?rio de Quimioterapia Experimental em Parasitologia Veterin?ria (LQEPV) from the Departamento de Parasitologia Animal of the Institute of Veterinary, Universidade Federal do Rural do Rio de Janeiro (UFRRJ) located in the municipality of Serop?dica. Growth regulators represent a new category that has been used widely in the control of ectoparasites of small animals. This group has no chemical effect "knockdown", acts slowly and gradually interfering with their growth and development. The AGR's were divided according to their mechanism of action on juvenile hormone analogues, benzoylphenyl ureas - acting in the inhibition of chitin synthesis inhibitors and deposition of chitin. The pyriproxyfen is one of the smallest juvenile hormone analogues marketed as topical treatment for the prophylactic control of fleas on dogs and cats. The experiment was divided into three stages, and in each of 24 rabbits were divided into control group, group treated with 1% pyriproxyfen treated with pyriproxyfen 1.5% and treated with 2% pyriproxyfen. The stage I (stage adult) rabbits were infested with 25 pairs of adult R. sanguineus per rabbit. In stage II (stage larvae) the rabbit were infested with larvae of R. sanguineus at a rate of 2300 larvae / rabbit. In the stage III (nymph stage), the rabbit were infested with 200 nymphs / rabbit. The dose of pyriproxyfen applied in the form "pour-on" at all stages was 10ml/kg body weight, and 0.5 ml in each ear and the rest on the back of the animal. Trials during the parasitic phase were conducted in environmental conditions in the period from February to August 2008, and the stage is not parasitic in controlled laboratory conditions (27 ? 1? C and 80 ? 10% RH in scotophase). We analyzed parameters of the parasitic phase and not parasitic to the larvae, nymphs and adults. The pyriproxyfen caused changes in the biology of R. sanguineus such as decrease in the recovery of evolutionary forms, reduction in weight of the egg mass, increased during the incubation period of eggs, decreased reproductive efficiency rate, inhibition of moulting between stages from larva to nymph, effective in inhibiting reproductive behavior of females and lethargic, but no concentration was observed in action on nymphs. It is concluded that focuses on employee pyriproxyfen at a concentration of 2% was effective in controlling rabbits and promoted behavioral changes in R. sanguineus that indicate the possibility of employment of AGR to control this tick, with the difference in reducing the rate of reinfestation. However, new studies should be carried out on the efficacy of pyriproxyfen to 2% in the control of R. sanguineus when used in dogs. The use of rabbits was a preliminary test to indicate whether or not the activity of pyriproxyfen on the tick R. sanguineus. / A realiza??o deste estudo teve como objetivo avaliar as altera??es morfol?gicas e biol?gicas apresentadas em diferentes fases evolutivas de Rhipicephalus sanguineus submetidos ao regulador de crescimento de artr?podes (AGR?s), piriproxifen, em coelhos tratados. O trabalho foi realizado nas depend?ncias do Laborat?rio de Quimioterapia Experimental em Parasitologia Veterin?ria (LQEPV), pertencente ao Departamento de Parasitologia Animal do Instituto de Veterin?ria da Universidade Federal Rural do Rio de Janeiro (UFRRJ), localizada no munic?pio de Serop?dica. Os reguladores de crescimento representam uma nova categoria que vem sendo empregada amplamente no controle de ectoparasitos de pequenos animais. Este grupo qu?mico n?o apresenta efeito ?knockdown?, atua de forma lenta e gradual interferindo no seu crescimento e desenvolvimento. Os AGR?s foram divididos de acordo com o seu mecanismo de a??o, em an?logos do horm?nio juvenil, benzoilfenil ur?ias - atuando na inibi??o da s?ntese de quitina e inibidores da deposi??o de quitina. O piriproxifen apresenta uma das menores estruturas qu?micas dentre os an?logos dos horm?nios juvenis introduzido no mercado como tratamento t?pico para o controle profil?tico de pulgas em c?es e gatos. O experimento foi dividido em 3 etapas, sendo que em cada uma delas utilizados 24 coelhos, divididos em grupos controle, grupo tratado com piriproxifen a 1%, tratado com piriproxifen a 1,5% e tratado com piriproxifen a 2%. A etapa I (fase de adultos) os coelhos foram infestados com 25 casais de adultos de R. sanguineus por coelho. Na etapa II (fase de larvas) os coelhos foram infestados com larvas de R. sanguineus na raz?o de 2300 larvas/coelho. Na etapa III (fase de ninfa), os coelhos foram infestados com 200 ninfas/coelho. A dose de piriproxifen aplicada sob a forma ?pour-on? em todas as etapas foi de 10mL/kg de peso vivo, sendo 0,5mL em cada orelha e o restante no dorso do animal. As experimenta??es durante a fase parasit?ria foram realizadas em condi??es ambientais, no per?odo de fevereiro a agosto de 2008, e da fase n?o parasit?ria em condi??es controladas de laborat?rio (27 ? 1 ?C e 80 ? 10% UR, em escotofase). Foram analisados par?metros de fase parasit?ria e n?o parasit?ria, para larvas, ninfas e adultos. O piriproxifen causou altera??es na biologia de R. sanguineus tais como: diminui??o na recupera??o das formas evolutivas, diminui??o no peso da massa de ovos, aumento no per?odo de incuba??o dos ovos, diminui??o do ?ndice de efici?ncia reprodutiva, inibi??o do processo de ecdise entre os est?gios de larva para ninfa, efic?cia na inibi??o da reprodu??o das f?meas e comportamento let?rgico, no entanto em nenhuma concentra??o foi observada a??o sobre ninfas. Conclui-se que o piriproxifen empregado na concentra??o de 2% foi eficaz no controle de R. sanguineus em coelhos, promovendo altera??es comportamentais indicando a possibilidade de emprego deste AGR no controle desse carrapato, com a particularidade na redu??o da taxa de reinfesta??o. Entretanto novos estudos necessitam ser realizados visando a efic?cia do piriproxifen a 2% no controle de R. sanguineus quando empregado em c?es. O uso de coelhos foi um teste preliminar para demonstrar a atividade do piriproxifen sobre o carrapato R. sanguineus

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