The aim of this diploma thesis is interior design of cosmetic studio located in the basement of family house. The theoretical part deals with the creation of public interiors, formation of new interior and all demands placed on the premises. The practical part includes interior design, which places emphasis on functionality, aesthetic quality and simultaneously takes into account with client´s requirements. In the practical part is also processed design of atypical furniture element, including its structural solution.

This master thesis deals with the assessment of selected fragrant substances in cosmetic products. At the beginning the occurrence, methods of acquiring and application of these substances is described. They are essential components primarily in cosmetic products, however they can cause an allergic reaction in sensitive individuals. The theoretical part of this thesis also describes the cause and the progress of these undesirable effects. There are several hundreds, up to thousands fragrant substances used in the perfume industry, from which 26 was designated by the European regulations as potential allergenic fragrant substances and their content is regulated. Legislative standards as well as the overview of the methods used to determine these fragrances are given here. The experimental part is focused on the development and validation of the separation methods for determining of the selected and potentially allergenic fragrant substances. The method used was a solid phase microextraction using Carboxen/Polydimethylsiloxane fiber combined with a gas chromatography coupled to a flame ionization detector (SPME-GC-FID). Amylcinnamyl alcohol, benzyl alcohol, cinnamyl alcohol, coumarin, lyral, ?-isomethyl ionone were among the determined allergenic substance. Consequently, this method was also applied on 10 selected cosmetic products specimens, in which the linalool and geraniol were identified as the most common allergenic substances.

The theoretical part of the thesis is focused on the basic description of the physiology of the skin, human microbiome and a brief summary of where individual microorganisms occur. Furthermore, there is a list of analytical and microbiological methods that are used in this thesis. In the beginning, the practical part is focused on determination of antimicrobial effects of the chosen cosmetic products using microbial tests. Then, the inhibiton and microbial effect of the chosen cosmetic products on examined microorganisms has been measured using ELISA method. The second part of the thesis is focused on the isolation of bacterial DNA in quality that would be high enough to be used for amplification in PCR. There has been an optimalization of isolation of microbial DNA that was to be found on tested subjects’ skin. The presence of chosen microorganisms on skin before and after the usage of cosmetic products was measured using a PCR method. PCR products were then detected using gel electrophoresis. From the gathered data it is clear that the number of observed microorganisms has changed significantly after the application of cosmetic products.

Hemp belongs among important plants with a long industrial tradition. However, its first use was originally for healing. In medicine, it is mainly applied thanks to the content of many biologically active substances, such as cannabinoids. This thesis is concerned with the use of hemp in acne preparations. The theory focuses mainly on the characterization of hemp and its active substances. The other chapters deals with cosmetic preparations and acne itself, and Propionibacterium acnes as well, which is involved. Within the experimental part, the extracts of two hemp varieties (Finola and Bialobrzeskie) were characterized in terms of antioxidant and cannabinoid presence. The cosmetic preparations with hemp extract content were prepared including a complete cleansing set for acne skin, concurrently commercial products of this sort were purchased. In cosmetic preparations and the extracts their inhibitory effect against Propionibacterium acnes was studied as well. Also Micrococcus luteus, Escherichia coli and Candida glabrata were tested. The results show that dried hemp flower extracts contain the greatest amount of active substances, so they have a higher antioxidant and antimicrobial effect. The presence of individual cannabinoids differed mainly depending on the variety. All hemp extracts had effect against gram-positive bacteria, in cosmetic preparations it was lower. Nevertheless hemp antibacterial effect has been proven.

Probiotics products are an integral part of the current market. Products containing probiotics cultures are also cosmetic products. The first part of the study focuses on testing of bacterial survival abilities in the environment of preservatives presented in cosmetic products. Collection strains of genus Lactobacillus were used for these tests. Another part of the study focuses on isolation of bacterial DNA from probiotic cosmetic products Ryor, Yoghurt of Bulgaria, FeminaMed and Lactovit Activit in PCR-ready quality. DNA was isolated by fenol extraction and with magnetic particles. Presence of bacteria was proved by genus and species specific PCRs Lactobacillus. Species specific PCR for identification of Lactobacillus pentosus was optimalized. Species identification was in accord with data declared by producers.

The aim of this thesis, which is called "Organization of production and quality in the company" was to assess how the management and organization help to abide by the production quality. There was introduced a production process of two chosen products, a process of the production flow and a background of the production in the selected company. Next part includes the description of the quality management of the chosen products including requirements and standards which are necessary for the production of the cosmetic products. Based on the findings of deficiencies, there were suggested measures to improve the quality management system.

The aim of this paper is to evaluate the loyalty system of Lancôme Beauty Institute and ascertain with the use of questionnaire investigation the satisfaction of customers with offered benefits and alternatively suggest the possibilities of improvement of the system. The theoretical part is focused on the general definition of customer satisfaction. The important part is recruitment, maintaining and motivation of current and potential customers. The practical part describes the functioning of the loyalty system of Lancôme Beauty Institute, the stages of development and implications of changes in the loyalty system. The questionnaire investigation was used to ascertain the satisfaction with loyalty system as well as to find the main imperfections -- the data were gathered, classified and analysed.

This thesis is focused on aroma compounds which are presented in a wide variety of products including perfume, cologne, cosmetics, toiletries, laundry products and detergens. The widespread use and exposure to aroma compounds of aforementioned products could cause a range of the adverse reactions such as contact dermatitis, asthma, eczema and breathing problems. There are a lot of aroma compounds triggering negative allergic reactions but EU regulations limit 26 the most important fragrance allergens. The presence of these substances has to be declared on the product label if a limit of 0,01 % for rinse off and 0,001 % for leave-on products is exceeded. Of these 26 substances, a methodology was elaborated for determination of 12 allergens in a experimental part of this thesis. A method by solid-phase microextraction (SPME) and gas chromatography-flame ionization detection (GC-FID) was applied for fragrance allergens identification, extraction and measurement. The optimal extraction conditions of SPME-GC method were investigated including equilibrium time, extraction time and extraction temperature to increase extraction efficiency. Another important point is the method by SPME-GC-FID was validated and following performance parameters were determined: repeatability, linearity, limit of detection (LOD) limit of quantitation (LOQ). SPME-GC-FID was tested and applied to real samples.

This diploma thesis is focused on the study of the influence of the of size cosmetics pigments on the properties of the final product. This effect was studied on samples of make-up given by industry partner. Make-up samples were measured by the rheology method and the yield stress of all samples was determined. Scanning electron microscopy was used to determine the size and shape of the pigment particles. Particle size was also measured by dynamic light scattering, but the results were influenced by forming aggregates. To easier determination of the types of pigments in the sample was determined elemental composition of the pigments (Ti, Fe, O, Al) by EDS detector. Due to the unknown composition of make-up, the information was supplemented by X-ray powder diffraction (XRD) analysis, and it was possible to determine the compounds TiO2 (anatase and rutile), FeOOH and Fe2O3. The results obtained by these methods showed what pigments can be found in make-up samples, what their sizes and shapes are. It has been shown that the smaller the pigments are, the higher the viscosity and yield stress of the final product. The higher these quantities, the harder it is to handle the product.

The present diploma thesis is focused on the selected anti-aging substances in cosmetic products. The literature research provided us with the knowledge of essential skin characteristics and factors influencing the aging process. Furthermore, the thesis describes the basic physical forms of cosmetic products, the basic cosmetic materials and the current trends in the usage of anti-aging substances. The experimental part subsumes altogether four physical forms of cosmetic products – tonics, serums, masks and creams. In this work was testid a new anti-aging compound, developer by French company SOLABIA, which provided the company Miša a Harašta s.r.o. (M+H). It has tested four components: Fucogel, Camaderm Gly, Viniderm and Omega Ceramid. These anti-aging substances were addend to the four technology cosmetic form, which were prepared in our laboratory. Subsequently, the pH stability of the products was measured together with the rheological measuring and skin hydration measuring. All of the samples proved to be pH stable. The highest stability of viscosity and consistence was identified for masks and creams. It was found out that the added anti-aging substances have no impact on the stability of pH, viscosity and consistency. The skin hydration measuring proved that the anti-aging substances Vinidem and Camadem Gly have the greatest moisturising qualities.