The aim of the thesis was analyse of sheep milk composition during lactation. Milk was analysed for milk yield, total solids, fat, protein, lactose, somatic cell coun (SCC), active acidity (pH), titratable acidity, rennet clotting time and rennet curd quality and then recorded data were satistically analyzed by program STATISTICA. The samplings were carried in months April – September 2018 on sheep farm in Br-níčko. The samplings were carried from eight selected ewes on the second lacatation and the immediately after collection the milk cooled, and transported to the analyses of in-dividual components into specialized laboratories. The average milk yield in the stage lacatation in 2018 was 1.39 l. Milk from these ewes contained an average of 18.41 % total solids, 7.01 % fat, 6.02 % protein, 4.71 % lactose, 103 thousand SC/ml. Milk showed values on average pH 6.63, titration acidity 9.51 °SH, rennetability 115 second and rennet curd quality was 1.34 (1.quality class). The results of this study indicate that high statistical significant effect of lactation stage was found on all chosen parametres except titration acidity, somatic cells count and rennet curd quality.