The objective of diploma work was comparing of different ways of cattle lacteal gland preparation before milking with reference to a milk quality, duration of selected ways of gland preparation and economic costs. There were gradually alleged various ways of gland preparations in parlour of selected agriculture company with breeding of Czech spotted dairy cows breeds {--} the wet, foam and dry way of cleanup. Selected ways of cleanup were applied for a period of three months. The monitoring proceeded in period since April till December 2007.