In this work, it is of interest to study the effects of thrombocytosis and microvascular anastomosis to that of vascular thrombosis. Here the splenectomy is used to the experimental subjects to have caused thrombocytosis first and later have the microvascular anastomsis on the divided vessel. Observations on different variables which may be related to vascular thrombosis are obtained during the three stages of the experiments, there are pre-splenectomy, post-splenectomy but before vascular division, and after the vascular anastomsis. These variables include platelet number, RBC count, Nitric oxide(nitrite and nitrate), femoral artery perfusion unit, hematocrit, reticulated-platelet number, and activated-platelets(p-selectin, CD62p). The experimental units are divided into thrombocysis group and control group. Basic statistical analysis are performed first to see the differences between the two groups for the above mentioned variables, and later try to use the profile analysis in multivariate analysis to understand the changes of these variables in different stages as well as their possible relationships with the cause of vascular thrombosis. Finally, classical regression and robust regression analysis for finding outliers are also used to see whether certain characteristics of those outliers have something to do with vascular thrombosis.