A typical "white" LED comprises a blue source and one or more phosphors, which down-convert the high energy blue light to longer wavelengths. The resultant "mixture" of wavelengths (predominantly residual blue light from the source and yellow and red from the phosphors) produces white light. Typically, phosphors are coated directly (proximally) onto the blue source. This work differs from the conventional approach by incorporating phosphors in a secondary optic, which is remote from the source. This has the advantages of being able to recover the source LED and to change the output wavelength by stacking optics that contain different levels of different phosphors, or by mixing different phosphors into a single optic. The work has created a range of secondary optics that contain one or more phosphors at relatively low levels of addition (1%-4% by weight) in thermoplastic and thermosetting matrices including polystyrene, poly(methylmethacrylate) and silicone. The phosphors are based on a range of YAGs and nitrides. The secondary optics were manufactured by injection moulding (thermoplastics) or casting (thermosets) and were used singly or in stacked layers to down-convert light from a royal blue (450 nm) LED source to produce a range of "white" light sources. Both injection moulding and casting are relatively inexpensive and commonly encountered processes. The optical performance of these optics was measured by Spectral Power Distribution (SPD), chromaticity, Colour Temperature (CCT) and Colour Rendering Index (CRI). CCT and CRI values ranged from 3000K to 6500K and (80-95) depending on the phosphor composition. Measurements were also carried out on the long-term performance of the secondary optics and these showed little or no change after 24 months continuous and intermittent exposure.

The purpose of this study was to investigate nurses’ roles within nurse-led chemotherapy clinics. There has been a rapid expansion and development of nursing roles and responsibilities in oncology, but little understanding of how roles are enacted and their impact on patient experiences and outcomes. This was a two stage approach comprising a survey of UK oncology specialist nurses followed by an ethnographic study of nurses’ roles in nurse led chemotherapy clinics. Ethics approval was obtained prior to each study; research and development approval was obtained from each hospital site prior to Study 2. Study 1 used a questionnaire survey to explore the scope of nurses’ roles. A purposive sample of oncology specialist nurses perceived to be undertaking nurse-led clinics was obtained using snowball methods. Data analysis included descriptive and inferential statistics. Study 2 used ethnographic methods to explore nurses’ roles in nurse-led chemotherapy clinics, which included clinical observations, interviews with nurse participants and studying documentation (protocols) for nurse-led chemotherapy clinics. Findings were coded and thematic analysis undertaken. In study 1, 103 completed questionnaires were received with a response rate of 64%, however analysis identified 79 (76.7%) nurses undertaking nurse-led clinics, therefore statistical analysis was limited to this sample of 79 nurses. An additional 12 (11.7%) nurses wanted to undertake nurse-led clinics, therefore findings from this group were analysed separately. There was little congruence between nurses’ titles and clinical roles, with significant differences in practice between different groups of nurses, in relation to history-taking (p=.036), assessing response to treatment (p=.033). Although there was no difference in the number of nurses undertaking clinical examinations (p=.065), there were differences in the nature of examinations undertaken, including respiratory (p= .002). There were also significant differences between groups of nurses in relation to nurse prescribing (p<.0001). Study 2 included observations (61 consultations by 13 nurses) and interviews (n=11). There was variability in patient numbers within nurse-led clinics, identifying implications for service delivery and sustainability. Disparities in nurses’ roles and responsibilities revealed four different levels of nurse-led chemotherapy clinics, from chemotherapy administration to totally nurse-led clinics. The identification of four levels of nurse-led chemotherapy is a new finding, and suggests a framework for nurse-led chemotherapy clinics that could link with nurse competencies and training. Five main themes were identified in study 2; a central theme of autonomy linked with themes of knowledge, skills, power and beliefs. A key finding was the reduced emphasis on compassionate care with greater medical (clinical) responsibilities within nurses’ roles, and poor communication skills by some nurses. Despite a great diversity in oncology specialist nurses’ roles, the lack of clarity in roles and responsibilities is creating confusion. Similarly the rapid increase in nurse-led chemotherapy clinics has been ad hoc with no formal evaluations. Although nurses in study 2 perceived they were providing holistic care there was no evidence of this in observations, and nurses appear to use a medical model care based on doctor-nurse substitution, which may have led to reduced emphasis on nursing skills and compassionate care.

Typically, in the semiconductor industry pn heterojunctions have been used as either light-emitting diodes (LED) or photodiodes by applying forward current bias or reverse voltage bias, respectively. However, since both devices use the same structure, the light emitting and detecting properties could be combine in one single device, namely LED-based photodetector. Therefore, by integrating LED-based photodetectors as either transmitter or receiver, optical wireless communication could be easily implemented for bidirectional visible light communication networks at low-cost. Therefore, this dissertation focus on the investigation of the photodetection characteristics of InGaN LED-based photodetectors for visible light communication in the blue region. In this regard, we obtain external quantum efficiency of 10 % and photoresponse rise time of 71 μs at 405-nm illumination, revealing high-performance photodetection characteristics. Furthermore, we use orthogonal frequency division multiplexing quadrature amplitude modulation codification scheme to enlarge the operational bandwidth. Consequently, the transmission rate of the communication is efficiently enhanced up to 420 Mbit/s in visible light communication.

The flavor oscillations of neutrinos due to the mixing of mass eigenstates have been thoroughly studied in several experiments. One missing piece of the puzzle is the mixing angle θ13, which is being searched for by the Daya Bay experiment. Currently, the experiment is still in construction mode. Part of the experiment involves building effective detectors for atmospheric muons, resulting in accurate detection of antineutrinos from the source. To ensure accurate detection, we must effectively calibrate the PMTs with the use of carefully chosen and calibrated LEDs. This thesis details the study of several LEDs measured in an attempt to determine the properties of the most likely source for our calibration efforts. I measured the spectra of the LEDs meant for use in calibration, along with several others for the purpose of comparison of spectrum width and to find the evidence of fluorescence in the LEDs. / Master of Science

The aim of the thesis is to introduce the basic photometric quantities and operating parameters of LED light sources, according to which the light sources are evaluated and compared. The thesis examines effects of high temperature and elevated stress on LED retrofits and a design of measurement methods to measure these influences. According to the proposed methods, nine samples of light sources were measured and compared to each other on a basis of the measurements. Comparison was made from the point of view of the energy as well as the quality of the produced light. The results were also compared to the parameters specified by the producers.

O objetivo neste estudo foi avaliar a velocidade de movimentação dentária no fechamento dos espaços durante a fase de retração em pacientes ortodônticos, submetidos a tratamento com extração dos primeiros pré-molares. Foram selecionados 18 pacientes sendo 9 homens e 9 mulheres, com idades entre 13 e 18 anos, alocados sequencialmente em dois grupos: LED (GLED) composto por dez pacientes (5 H, 5 M) que fizeram auto aplicação com aparelho de LED, durante 10 minutos diários, durante a fase de retração ortodôntica, por um período de 12 semanas e Controle (GC), composto por oito pacientes (4 H, 4 M) que não aplicaram o LED durante a mesma fase. O fechamento dos espaços das extrações foi obtido com mecânica de deslize com arcos de aço com calibre 0.019\" x 0.025\" e molas de níquel-titânio com 200g de força, ativadas a cada 4 semanas. A mecânica escolhida permitiu-nos analizar 36 quadrantes e não 18 pacientes e isto justifica o tamanho final da nossa amostra: GLED 20 quadrantes superiores e 16 quadrantes inferiores e GC 16 quadrantes superiores e 12 quadrantes inferiores. Na arcada superior a retração foi ancorada em mini-implantes ortodônticos, e na arcada inferior sem acessórios de ancoragem e por este motivo foram avaliadas separadamente. Para avaliar o fechamento dos espaços, após calibragem, um único operador realizou dois tipos de medições: clínicas e em modelos de estudo, com compasso de ponta seca e paquímetro digital modificado, considerando quatro variáveis consistentes em distância entre os dois dentes vizinhos ao espaço da extração. Os resultados mostraram quedo início (T0) ao término do período de observação (T3) não houve diferença estatísticamente significante entre os grupos, exceto na medição dos modelos da arcada inferior, nas variáveis 1,3 e 4, com maior redução dos espaços no GLED: GLED > GC. Observou-se também diferença significante entre os grupos apenas na variável 1 do GLED na medição dos modelos da arcada inferior, no sentido de maior velocidade entre T1 e T2. Não houve diferença estatística entre os tipos de medidas realizadas clinicamente e nos modelos de estudo. Independentemente da aplicção ou não do LED obdservou-se uma significativa redução dos espaços ao longo do tempo em ambos os grupos, tanto da arcada superior, como na arcada inferior, que permitiu o sucesso do tratamento ortodôntico. Com base nos resultados pôde-se concluir que o protocolo de aplicação do LED utilizado na casuística estudada não caracterizou diferencialmente os grupos estudados. Porém, por ser um método novo na avaliação da influência sobre a movimentação dentária, sem dados na literatura para comparar os nossos, faz-se necessário a realização de estudos futuros que testem protocolos de mecânica ortodôntica e aplicação de LED. / The aim of this study was to evaluate the speed of tooth movement in space closure during the retraction stage, in orthodontic patients submitted to treatment with first premolar extractions. Eighteen (18) patients undergoing orthodontic treatment were selected, of whom 9 were men, and 9 women, age-range between 13 and 18 years, sequentially allocated to two groups. LED (GLED) composed of ten patients (5 M, 5 W) who performed self-application with a LED appliance, for 10 minutes daily, during the stage of orthodontic retraction, for a period of 12 weeks, and the Control Group (GC), composed of eight patients (4 M, 4 W) who did not apply LED during the same stage. Space closure of the extractions was obtained with sliding mechanics with steel archwires, caliber 0.019\" x 0.025\" and nickel-titanium springs with 200 g of force, activated every 4 weeks The mechanics chosen allowed the authors to analyze 36 quadrants and not 18 patients, and this justified the final size of our sample: GLED 20 maxillary quadrants and 16 mandibular quadrants; and GC 16 maxillary quadrants and 12 mandibular quadrants. In the maxillary arch, retraction was anchored on orthodontic mini-implants and in the mandibular arch, no anchorage accessories were used; and for this reason, they were evaluated separately. To evaluate space closure, after calibration a single operator performed two types of measurements: clinical, and in study models, by using a dry tip compass and modified digital pachymeter, considering four variables of distances between the two neighboring teeth, compatible with the extraction space. The results showed that from the beginning (T0) up to conclusion of the observation period (T3) there was no statistically significant difference between the groups, except for the measurements of the mandibular arch models, in the variables 1,3 and 4, showing greater reduction of the spaces in GLED: GLED > GC. The authors also observed significant difference between the groups only in variable 1 of GLED, in the measurements of the mandibular arch models, showing greater velocity between T1 and T2. There was no statistical difference between the types of measurements performed clinically and those in the study models. Irrespective of the application, or no application of LED, a significant reduction was observed in the spaces over time in both groups, both in the maxillary and mandibular arches, which allowed the orthodontic treatment to be successful. Based on the results, the authors could conclude that the LED application protocol used in the casuistic studied did not characterize the groups studied in a differential manner. However, since this was a new method for evaluating the influence on tooth movement, without data in the literature to compare with those of the authors, it is necessary to conduct future studies that test the orthodontic mechanics and LED application protocols.

A restauração de resina composta dental possui grande aplicação clínica. A polimerização da resina composta é feita através de um processo de fotoativação, que influenciará diretamente as propriedades finais do material restaurador. O estudo e o desenvolvimento das técnicas e dos dispositivos de fotopolimerização das resinas são fatores de grande relevância para melhorar a eficiência do uso da resina composta na Odontologia. Neste trabalho foram avaliadas três fontes de luz, laser de argônio e diodo emissor de luz azul (LED), em comparação com uma lâmpada de fotopolimerização convencional. Através do estabelecimento do diagrama PTT (Potência- Tempo-Temperatura), foram estabelecidos parâmetros seguros de utilização do laser de argônio, para evitar danos térmicos irreversíveis ao tecido pulpar. Na avaliação da dureza, em função da profundidade de penetração da luz no material, foram estabelecidos os parâmetros de operação do laser de argônio para a obtenção de valores de dureza semelhantes ou melhores aos obtidos com a lâmpada convencional. A microinfiltração, em restaurações classe V polimerizadas com o laser e a lâmpada convencional, não apresentou diferença estatisticamente significante quando comparadas às duas fontes de luz investigadas. A elaboração dos dispositivos à base de LEDs, assim como o estudo da dureza da resina composta curada, possibilitou uma análise do processo de cura e a determinação de parâmetros corretos de utilização dos arranjos de LEDs estudados. Nosso estudo termina com a proposta de um dispositivo alternativo para a fotoativação das resinas compostas. / The restorations based on composite resin have great application in modem dentistry. The composite polymerization is obtained by a photoactivation process. The final properties of materials are connected with the cure process. The development of photopolymerization procedures and devices is important to improve the cure efficiency and its quality. In this work, two light sources, argon laser and blue light-emitting diodes (LEDs), were studied and compared to a conventional cure lamp. In the PTT (Power-Time-Temperature) diagram it were established safe working parameters to argon laser application, in order to avoid pulpal damage. The microhardness was used to quantify mechanical properties. The observation of VHN as a function of the depth in the material, allowed us to determine the operational parameters where argon laser produces similar properties compared with the conventional lamp. The microleakage observed in class V restorations cured by laser or conventional lamp was investigated and the result did not show statistical difference when the two light sources were compared. Using the devices based on LEDs it was possible to study the cure process and establish the operational parameters when this alternative light source is considered for use in composite resin cure.

A restauração de resina composta dental possui grande aplicação clínica. A polimerização da resina composta é feita através de um processo de fotoativação, que influenciará diretamente as propriedades finais do material restaurador. O estudo e o desenvolvimento das técnicas e dos dispositivos de fotopolimerização das resinas são fatores de grande relevância para melhorar a eficiência do uso da resina composta na Odontologia. Neste trabalho foram avaliadas três fontes de luz, laser de argônio e diodo emissor de luz azul (LED), em comparação com uma lâmpada de fotopolimerização convencional. Através do estabelecimento do diagrama PTT (Potência- Tempo-Temperatura), foram estabelecidos parâmetros seguros de utilização do laser de argônio, para evitar danos térmicos irreversíveis ao tecido pulpar. Na avaliação da dureza, em função da profundidade de penetração da luz no material, foram estabelecidos os parâmetros de operação do laser de argônio para a obtenção de valores de dureza semelhantes ou melhores aos obtidos com a lâmpada convencional. A microinfiltração, em restaurações classe V polimerizadas com o laser e a lâmpada convencional, não apresentou diferença estatisticamente significante quando comparadas às duas fontes de luz investigadas. A elaboração dos dispositivos à base de LEDs, assim como o estudo da dureza da resina composta curada, possibilitou uma análise do processo de cura e a determinação de parâmetros corretos de utilização dos arranjos de LEDs estudados. Nosso estudo termina com a proposta de um dispositivo alternativo para a fotoativação das resinas compostas. / The restorations based on composite resin have great application in modem dentistry. The composite polymerization is obtained by a photoactivation process. The final properties of materials are connected with the cure process. The development of photopolymerization procedures and devices is important to improve the cure efficiency and its quality. In this work, two light sources, argon laser and blue light-emitting diodes (LEDs), were studied and compared to a conventional cure lamp. In the PTT (Power-Time-Temperature) diagram it were established safe working parameters to argon laser application, in order to avoid pulpal damage. The microhardness was used to quantify mechanical properties. The observation of VHN as a function of the depth in the material, allowed us to determine the operational parameters where argon laser produces similar properties compared with the conventional lamp. The microleakage observed in class V restorations cured by laser or conventional lamp was investigated and the result did not show statistical difference when the two light sources were compared. Using the devices based on LEDs it was possible to study the cure process and establish the operational parameters when this alternative light source is considered for use in composite resin cure.

Em uma viagem de avião os passageiros ficam de 1 a 12 horas em determinada localização, na maior parte do tempo sentados, com limitações nos ajustes de seus assentos, expostos a ruídos, vibrações mecânicas, temperaturas desconfortáveis, condições que podem levá-los a um processo de estresse em função do desconforto a que estão sujeitos. Outro fator importante para a sensação de desconforto é a qualidade e a cor da iluminação do interior da aeronave. A cor de uma iluminação tem influência no estado de humor das pessoas, que pode afetar de maneira positiva ou negativa o seu estado emocional. Com o avanço tecnológico e a invenção LED (Light Emitting Diode, Diodo Emissor de Luz) pode-se criar uma iluminação no interior da cabine, com cores que podem ser alteradas facilmente e dessa maneira influenciar o estado emocional dos passageiros. O objetivo da presente pesquisa é determinar a sensação de conforto/desconforto causada pela cor da iluminação em uma cabine de aeronave. Foi realizada uma pesquisa bibliográfica para se obter dados sobre a influência das cores no estado emocional das pessoas. Também foi pesquisado o mecanismo de funcionamento do sistema visual humano e como percebemos as informações visuais. A pesquisa foi realizada com uma população representativa de passageiros de aeronaves. Fez-se um levantamento quantitativo de aspectos subjetivos da percepção da qualidade do ambiente em função da cor da iluminação, por meio da aplicação da técnica do diferencial semântico. O ambiente de estudo desta investigação foi um mockup que reproduz o interior de uma cabine de aeronave em condições semelhantes a uma viagem de avião. Avaliou-se a influência de algumas características físicas dos passageiros, tais como gênero, cor dos olhos e Índice de Massa Corporal (IMC), na percepção da qualidade do ambiente em função da cor da iluminação. Os resultados das análises mostraram que as sensações percebidas do ambiente são afetadas pelas características físicas dos indivíduos, com diferenças significativas de percepção da qualidade do ambiente entre o sexo masculino e o feminino, entre IMC>25 e IMC<25, e entre olhos claros e olhos escuros. Pela análise dos resultados também se determinou cores mais adequadas para reduzir o estresse de uma viagem de avião e para cada fase do voo. O resultado encontrado apresentou um grau de incerteza elevado, com desvio padrão de 20 pontos em uma amplitude de valores de 0 a 100, o que evidencia a subjetividade das percepções. Este resultado revela que o ideal, em termos de conforto da cor da iluminação, é individualizar a iluminação. Além das possibilidades práticas de aplicação em cabines de aeronave, o resultado obtido poderá se estender a outras aplicações, em que se busca o bem estar propiciado por uma cor de iluminação adequada. / On a plane trip passengers could stay from 1 to 12 hours in a specific location, most of the time sitting with limitations related to the settings of their seats, subjected to noise, vibration, uncomfortable temperatures, conditions that can lead them to a stress due to the discomfort process that they are exposed. Another important factor for the discomfort factor is the quality and color of aircraft interior illumination. The color of illumination influences the people´s mood, which can positively or negatively affect their emotional state. With technological advancement and invention of LED (Light Emitting Diode) it is possible to create a lighting inside the cabin with colors that can be changed easily and thus influence the passengers´ emotional state. The aim of this research is to determine the sense of comfort / discomfort caused by the lighting color in an aircraft cabin. A literature survey was conducted to obtain data about the influence of colors on people´s emotional state. It was also investigated the mechanism of functioning of the human visual system and how we perceive visual information. The survey was conducted with a representative sample of passenger aircraft. It was made a data collection about subjective aspects of the environment perception due to the illumination color by applying the technique of semantic differential. The study environment of this research was a mockup that reproduced the interior of an aircraft cabin in conditions similar to a plane trip. It was assessed the influence of some physical passengers characteristics, such as gender, eyes color and body mass index (BMI), in the perception of the environment due to the illumination color. The analysis of the results showed that the perceived sensations of the environment are affected by the physical characteristics of the persons, and that there were significant differences in perception of the environment between male and female, between BMI > 25 and BMI < 25, and between light eyes and dark eyes. Through the analysis of the results it was also determined most appropriate colors to reduce the stress of traveling by plane and for each phase of flight. The results founded showed a high degree of uncertainty, with a standard deviation of 20 points in a range of values from 0 to 100, which demonstrates the subjectivity of perceptions. This result reveals that the ideal, in terms of comfort Illumination color, is to individualize the lighting. Apart from the practical possibilities of application in aircraft cabins, the result can be extended to other applications where one seeks the well being brought by a color proper lighting is seek.

Dentre os vários compostos utilizados na pesquisa e na terapia de doenças osteo-degenerativas, a fototerapia com laseres de baixa potência (LLLT) e os diodos emissores de luz (LEDs) vem sendo investigada com o intuito de avaliar seus efeitos no metabolismo ósseo. Estes, que possuem comprimentos de ondas específicos, atuam na biomodulação das células, funcionando como um agente terapêutico, reequilibrando e normalizando a sua atividade. No entanto, pouco se sabe sobre o efeito dos diferentes espectros na proliferação e diferenciação de osteoblastos humanos, bem como seus efeitos no metabolismo celular como a síntese e a ativação de proteínas sinalizadoras envolvidas nesses processos. Diante disso, o objetivo deste trabalho foi avaliar, comparativamente, a influência da fototerapia com LLLT e LED na proliferação e diferenciação de osteoblastos humanos. Além disso, investigamos o envolvimento da ativação da via de sinalização ERK1,2 nestas respostas, utilizando o seu inibidor específico e/ou avaliando a sua ativação durante a proliferação e após fototerapia. Para esse estudo, osteoblastos humanos (HOAL) foram cultivados em meio de cultura DMEM suplementado com 10% de soro fetal bovino (SFB) e incubados em estufa de CO2. As células foram irradiadas pontualmente com os laseres vermelho (660nm), infravermelho (780nm) e LED (637nm), nas doses de 10, 20 e 50 J/cm2 na potência de 40mW, após adesão celular. Após 24, 48, e 72 horas foram realizados os ensaios de redução do MTT (brometo de 3-(4,5-dimetiltiazol-2-yl)-2,5- difeniltetrazólio) e cristal violeta (CV) para avaliar a viabilidade das células e após 72 horas foi realizada a análise da proliferação por citometria de fluxo nos quais os resultados sugerem aumento de células viáveis ou proliferação quando estimuladas pelos diferentes espectros. Após a verificação do efeito positivo dos laseres e LED na viabilidade e/ou proliferação, foi realizada a análise da ativação da proteína intracelular ERK por western blotting usando anticorpo específico após 10 minutos da irradiação pontual. Mostramos que a irradiação das células HOAL com LLLT, na dose de 10 J/cm2, aumentou significativamente a fosforilação da ERK1/2 em relação ao controle. Para os ensaios de diferenciação, as células receberam pontualmente o estimulo a cada 6 dias e após os períodos de 07, 14, 21 e 28 dias foram realizados os ensaios da atividade de fosfatase alcalina (ALP), expressão gênica do colágeno I (COL1A1) e SPARC (osteonectina) por RT-PCR em tempo real e ensaio de mineralização com vermelho de alizarina. De um modo geral, não foram observados diferenças na atividade da ALP entre os grupos tratados em relação ao controle. A expressão gênica do COL1A1 e SPARC foi aumentada, no qual o LED em ambas doses apresentou maior eficácia em aumentar a expressão desses genes. No que se refere à mineralização, foram observadas pequenas diferenças quanto a deposição de cálcio. Dessa forma, a LLLT e LED, nesse regime de aplicação modulou positivamente o metabolismo dos osteoblastos humanos em relação à viabilidade, porém, no processo de mineralização foram observadas poucas diferenças. / Among the various compounds used in research and bone degenerative diseases therapy, phototherapy with low level laser (LLLT) and light emitting diodes (LEDs) has been investigated in order to evaluate its effects on bone metabolism. Those, who have specific wavelengths, act in biomodulation cells functioning as a therapeutic agent, rebalancing and normalizing their activity. However, little is known about the effect of the different spectra in the proliferation and differentiation of human osteoblasts and their effects on cellular metabolism as well as the synthesis and activation of signaling proteins involved in these processes. Therefore, the aim of this study was to compare the influence of LLLT and LED phototherapy in the proliferation and differentiation of human osteoblasts. In addition, we investigated the involvement of activation of ERK1,2 signaling pathway these responses using its specific inhibitor and/or evaluating their activation during the proliferation and after phototherapy. For this study, human osteoblasts (HOAL) were cultured in DMEM culture medium supplemented with 10 % fetal bovine serum (FBS) and incubated in CO2 incubator . Cells were irradiated with punctual red lasers (660nm), infrared (780nm) and LED (637nm) at doses of 10, 20 and 50 J/cm2 in power 40mW, after cell adhesion. After 24, 48, and 72 hours, MTT assay (- (4,5- dimethylthiazol-2- yl) -2,5 - diphenyltetrazolium bromide 3 ) and violet crystal (CV) were performed to assess the viability of cells and after 72 hours, was performed of proliferation analysis by flow cytometry. The results suggest an increase in viable and proliferation of cells when stimulated by different spectra. After checking the positive effect of lasers and LED viability and/or proliferation, analysis of ERK activation of intracellular protein by western blotting using a specific antibody was performed 10 minutes after the spot irradiation. We show that irradiation of HOAL cells with LLLT at a dose of 10 J/cm2, significantly increased the phosphorylation of ERK1/2 compared to control. For differentiation assays, cells promptly received stimulation every 6 days and after periods of 07, 14, 21 and 28 days testing the activity of alkaline phosphatase (ALP), gene expression of type I collagen (COL1A1) and SPARC (osteonectin) were performed by Real Time RT-PCR and mineralization experiment with alizarine red. In general, no differences in the activity of ALP between the treated groups compared to the control were observed. The gene expression was increased, and SPARC COL1A1, where in the LED in both doses showed greater effectiveness in increasing the expression of these genes. With respect to mineralization, small differences in the deposition of calcium were observed. Thus, LLLT and LED, this enforcement regime, positively modulated the metabolism of human osteoblasts during the cell viability, but in the process of mineralization few differences were observed.