The Chandeleur Islands (Louisiana) contain nursery habitats for lemon sharks that provide abundant prey and protection from predation. Other local shark species (Atlantic sharpnose, bull, and blacktip sharks) co-occur with lemon sharks in the same region, including the nearby Biloxi Marshes. To better assess how lemon sharks use these nursery habitats, I measured diet and prey availability of young of the year and juvenile lemon sharks from 2009 to 2010. Young lemon sharks at the Chandeleur Islands have a relatively reduced diet breadth in comparison to those from nurseries in Bimini (Bahamas) and the Florida Keys. At the Chandeleur Islands, young lemon sharks appear to be opportunistically feeding on the most abundant prey items, resulting in high prey abundance and low diversity in their diet. Opportunistic feeding by young lemon sharks suggests minimal dietary overlap with other local shark species, resulting in minimal competition for resources.

Anecdotal evidence suggested that immature lemon sharks (Negaprion brevirostris) occur at the Chandeleur Islands in southeastern Louisiana. From May 2009 to August 2011, the Nekton Research Laboratory at the University of New Orleans regularly sampled habitats at the Chandeleur Islands to confirm the presence of immature individuals of this species. During these efforts, 147 immature lemon sharks (neonates, young-of-the-year, and juveniles) were collected. Each shark was PIT tagged and a tissue sample was taken for genetic analyses. Sizes ranged from 56 – 177 cm total length. Sharks showed a preference for sandy substrate and smaller individuals showed significant preference for shallow waters. Collection data for N. brevirostris were obtained from various locations around the Gulf of Mexico for comparison. Based on a review of current literature, collection data, and microsatellite analysis it was possible to classify the Chandeleur Islands as an elasmobranch nursery ground under the definition presented by Huepel et al. (2007). Current literature and high densities of N. brevirostris suggests that the Chandeleur Islands are the only area in the northern Gulf of Mexico where neonatal and YOY lemon sharks have been recorded. Results from a combination of SPOT 5 satellite tags, PIT tags, and FLOY tags show that immature N. brevirostris at the Chandeleur Islands remain in the vicinity of the islands for several months. The abundance of juvenile sharks across years provides evidence of continuous use of these islands as a pupping area. Genetic analysis supports this information with 7 of 15 mothers returning across years. The combination of this information indicates that the Chandeleur Islands are the northern- and western-most documented nursery ground for this species. Special care should be given to this habitat as CPUE for N. brevirostris decreased significantly during this research following the 2010 Deepwater Horizon disaster and subsequent oil prevention measures. Genetic research indicates a high level of inbreeding and genetic separation between the Chandeleur Islands population and other known populations in Florida, Belize, and the Bahamas.

Habitat use of mesophotic coral reefs by sharks is largely unknown. However, it is well established that mesophotic reefs are the site of spawning aggregations for many species of teleost fish. These aggregations represent seasonal concentrations of potential prey biomass that may influence the habitat use of predatory species such as large sharks. I employed acoustic monitoring to examine the movements of three shark species [lemon shark (Negaprion brevirostris), tiger shark (Galeocerdo cuvier), and Caribbean reef shark (Carcharhinus perezi)] to determine 1) the comparative spatiotemporal patterns of mesophotic reef habitat use by the three shark species and 2) the spatiotemporal relationship between these sharks and grouper spawning aggregations at a fish spawning aggregation (FSA) site (Hind Bank and Grammanik Bank) along the southern reef shelf edge off St. Thomas, United States Virgin Islands (USVI). Tiger and lemon sharks were detected across nearly the entire acoustic array, which spanned ~ 1060 km2. When present, Caribbean reef sharks used a much smaller activity space, composed exclusively of mesophotic reef habitat located within FSA sites. Individuals from all three species were typically detected for stretches of several consecutive days, while periods without detections usually lasted less than one week. Lemon sharks were present at the FSA site more often during the grouper spawning season (Dec-May) than the non-spawning season (Jun-Nov), but showed no preference toward specific areas within the FSA site, which varied by location and grouper species composition. In contrast, there was no relationship between the presence of tiger and Caribbean reef sharks at the FSA site and the grouper spawning season. My results suggest that despite different habitat use 5 patterns and varying degrees of fidelity, this mesophotic reef serves as an important habitat to all three shark species.

Habitat use of mesophotic coral reefs by sharks is largely unknown. However, it is well established that mesophotic reefs are the site of spawning aggregations for many species of teleost fish. These aggregations represent seasonal concentrations of potential prey biomass that may influence the habitat use of predatory species such as large sharks. I employed acoustic monitoring to examine the movements of three shark species lemon shark (Negaprion brevirostris), tiger shark (Galeocerdo cuvier), and Caribbean reef shark (Carcharhinus perezi)] to determine 1) the comparative spatiotemporal patterns of mesophotic reef habitat use by the three shark species and 2) the spatiotemporal relationship between these sharks and grouper spawning aggregations at a fish spawning aggregation (FSA) site (Hind Bank and Grammanik Bank) along the southern reef shelf edge off St. Thomas, United States Virgin Islands (USVI). Tiger and lemon sharks were detected across nearly the entire acoustic array, which spanned ~ 1060 km2. When present, Caribbean reef sharks used a much smaller activity space, composed exclusively of mesophotic reef habitat located within FSA sites. Individuals from all three species were typically detected for stretches of several consecutive days, while periods without detections usually lasted less than one week. Lemon sharks were present at the FSA site more often during the grouper spawning season (Dec-May) than the non-spawning season (Jun-Nov), but showed no preference toward specific areas within the FSA site, which varied by location and grouper species composition. In contrast, there was no relationship between the presence of tiger and Caribbean reef sharks at the FSA site and the grouper spawning season. My results suggest that despite different habitat use patterns and varying degrees of fidelity, this mesophotic reef serves as an important habitat to all three shark species.