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The paper evaluates technological, economic and construction aspects of the component production and the design of the tool serving for large-scale production of the S355MC steel rack component. Use of a progressive tool combining cutting and bending operations in four steps has been decided as the most appropriate according to literature study. Based on the need of production of two plane-symmetrical pieces at a time, the tool will produce both pieces simultaneously, which is economically advantageous. The tool is designed for use on the Arisa 630t mechanical press. Material will be winded off the roll, flattened and pushed into the tool by a precision feeder. The total cost of production of the component was set at 18.80 CZK.

The master’s thesis deals with analysis of sealing elements of Wankel engines and their design. The necessary steps for creation of the model of dynamics of apex seal are provided, whereby the resulting force loads are then obtained. Furthermore, the design of the apex seal spring, the choice of the material and the production jigs for the production of the spring itself are presented. The finished components are tested in the engine testing laboratory and the results evaluated.

This thesis deals with the analysis and design OF self-propelled mower on the grass. The machine is designed for cutting fodder, and their subsequent treatment. Focusing on the vast scope and great hourly output. The design emphasis is on compliance ergonomic rules, easy maintenance and functionality of the machine. For a very innovative features can be seen ejecting the cab, crop spacing on the sides, drive hydraulic cutter bars. In particular, the location of the cabin and boarding colors and folding machine cutter bars during transport. The machine was created to perfect performance, operator comfort, performance focused on large cysts and fluid design.

My PhD thesis has addressed specific questions regarding cell proliferation and tissue dynamics in three key areas of craniofacial development: during suppression and revitalization of tooth buds that develop in the mouse embryonic dentition as rudiments of lost premolars; during dental papilla and follicle formation of the first mouse molar; and during origin of palatal rugae on the mouse hard palate. By evaluation of cell proliferation, we recorded a change in the proliferation pattern along the cheek region of the mandible between less and more advanced embryos at embryonic day 13.5. Thus during the time period, when the development of the large mouse rudimental premolar primordium (R2) is stopped, we showed that the arrest of the rudiment R2 is caused by exhibiting low rate of cell proliferation and high rate of apoptosis. When Sprouty gene signalling is disrupted, the premolar primordium shows rates of proliferation and apoptosis similar to the growing first molar. The R2 subsequently revitalizes and develops into a supernumerary tooth in front of the first molar. Furthermore, we discovered that the dental mesenchyme is very dynamic tissue during bud and cap stages of tooth development and that the dental papilla of the first molar originates only from a restricted region of the dental...

This work describes the rationalization process in Lotus Notes in a company that provides complete support for Lotus Notes Product for a large international company. It describes three level support and also deals with the rationalization process between the first level of support (customer service) and second level support (Lotus Notes administration). Finally, describes how to create – programming the agents in the Lotus Notes team for the second level, which will help to speed up and improve the services provided by this team.

Differentiation of the head ectoderm is crucial for the evolutionary diversification of vertebrates. Expression of the genes responsible for this process is orchestrated troughout complex gene regulatory networks that are induced and modulated by Wnt, FGF and BMP signaling pathways. In addition, Wnt/β-catenin signaling, in combination with expression of the Wnt antagonists from the rostral-most part of the head ectoderm, represent a key source of information for the regionalization of the tissue along the antero-posterior axis. This allows the differentiation of the anterior ectoderm that gives rise to the anterior neural fold (ANF) and anterior part of the presumptive placodal region (PPR), and more posterior ectoderm where higher levels of active Wnt/β-catenin signaling promote differentiation into the neural crest (NC) and posterior PPR. Although the requirement of Wnt/β-catenin signalling for ANF, PPR and NC development has been intensively studied in non-mammalian vertebrate model organisms, we lack a clear picture about the situation in mammals. Furthermore, current knowledge in mammals has been gathered via experiments on the level of β-catenin and very little is known about the individual roles of the Tcf/Lef transcription factors. Thereby, we decided to manipulate the Tcf7l1, member of the...

Despite the fact that some aspects of single studies differ, there's a generally accepted view that the whole cartilaginous viscerocranium of vertebrates is neural crest derived. By the series of isotopic transplantation experiments of presumptive neural crest on the model organism Ambystoma mexicanum I partly specify this oppinion and prove that the most ventro-caudal cartilage, the second basibranchial, is of a different origin. Furher I mention the level of the presumptive neural crest where the single parts of cartilaginous viscerocranium arise from. Moreover there is one element, the first basibranchial, which has double origin. I discuss also some other neural crest derivatives such as head and outer gills mesenchyme, the trabeculae cranii, part of the cartilaginous otic capsule or the connective tissue in the head. I have performed 179 transplantations between transgenic and normal axolotl embryos. My final analysis is composed of 65 embryos of stage 40 - 42 and 7 larvae of lenght of 15 - 17 mm.

Extensively migrating population of neural crest cells, which contributes to many tissues and builds up most of craniofacial vertebrate structures, has a crucial role in embryonic development of vertebrate body. The migratory pathways of neural crest cells are thought to be very conserved throughout the vertebrates and cranial neural crest migration is defined by progression of three migratory streams: trigeminal, hyoid and a common branchial stream. In this diploma thesis, migration of cranial neural crest was analysed using embryos of the Senegal bichir (Polypterus senegalus) and of sterlet (Acipenser ruthenus), which represent two basal-most lineages of extant ray-finned fishes. A combination of several techniques was used in both species in order to study cranial neural crest cells from their sites of origin to post- migratory stages and the pattern of migration was compared and discussed in revealed embryonic context. In the Senegal bichir the hyoid neural crest stream was shown to migrate first and it is also the most abundant; this heterochrony shift is apparently related to formation of external gills, which in bichir are situated on the hyoid arch only. In sterlet, neural crest cells migrate in a classic pattern of three progressive streams but their dynamics and patterning is influenced by...

The master's thesis is focused on a design of optimal working speed of auger and belt transporter which are parts of Power Flow header for standard type of combine harvester. In the master's thesis author suggest a new simplified theory of crop flow inside the Power Flow header. The thesis contain the factors which can affect the crop flow and comparison between the Power Flow and competitor's header. The thesis also contain geometrical characteristics of crop for four types of cereal.