Taiwan Island is surrender by ocean; the ninety percent of raw material for economic development depends on sea transportation. Thus, it is important to keep sea line of communication for using without any obstruction. As known, the most serious threat to sea line of communication of Taiwan is from submarine of PRC. However, the KNOX class frigate is the warship which is designed for ASW especially by USN. She is still the major ASW ship for Taiwan navy so far. For this reason, the officers working on the KNOX is the main objective to this case study for relationship between the job satisfaction and turnover intention. Consequently, the recommendation according to the study conclusion was provided. The method was used in this study were theory review, questionnaires, and statistics analysis which covered t-test, one-way ANOVA, Pearson product-moment correlation, and regression analysis. Accordingly, the study results are follows, A. The job satisfaction of officers was moderate and they were also precious to the current job without significant turnover tendency. B. For the demographic aspect, unexpectedly, female officers had higher job satisfaction than male ones. Unmarried officers had higher job satisfaction than married ones. The age between 26 and 30 years old officers had lower job satisfaction than others. C. The most significant influence of key factors on job satisfaction were career expectation, job perspective, fair reorganization, motivated strategy, and military culture. D. The negative relation was resulted from the job satisfaction and turnover intention of the KNOX officers.