Thesis (MScAgric (Conservation Ecology and Entomology))--Stellenbosch University, 2008. / ENGLISH ABSTRACT: Ant infestations comprising the Argentine ant Linepithema humile (Mayr), common pugnacious ant Anoplolepis custodiens (F. Smith) and cocktail ant Crematogaster peringueyi Emery are a widespread pest problem in South African vineyards. Integrated Pest Management (IPM) programmes aimed at suppressing the problematic honeydew excreting vine mealybug Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae) on grapes must include ant control to optimize the effectiveness and efficacy of mealybug natural enemies. If ants are eliminated, natural enemies are able to contain mealybugs below the Economic Threshold Level (ETL). Current strategies for ant control are limited and generally include the application of long term residual insecticides that are detrimental to the environment, labour intensive to apply and can disrupt natural biological control if applied incorrectly. A more practical method of ant control using low toxicity baits was therefore investigated. Field bait preference and bait acceptance assessments aimed at determining bait repellency and palatability, respectively, were carried out during spring, summer and autumn in three vineyards of the Cape winelands region during 2007/08. Five toxicants comprising gourmet ant bait (0.5%), boric acid (0.5%), fipronil (0.0001%), fenoxycarb (0.5%) and spinosad (0.01%) dissolved in 25% sugar solution were tested against a 25% sucrose solution control. Gourmet ant bait was significantly more preferred and accepted by all ant species than the other baits. Laboratory bait efficacy assessments using four insecticides (gourmet, boric acid & spinosad) at concentrations of 0.25; 0.5; 1; 2 and 4 times the field dose and fipronil at 0.015625; 0.03125; 0.0625; 0.125; 0.25 times the field dose were carried out. Results revealed that boric acid (2%), gourmet ant bait (2%) and fipronil (1.0 X 10-5%) exhibited delayed toxicity for L. humile and C. peringueyi while spinosad (0.01%) showed delayed action on L. humile. Field foraging activity and food preference tests were also carried out for the three ant species during 2007/08. Foraging activity trials revealed that vineyard foraging activity of L. humile is higher relative to A. custodiens and C. peringueyi. This means fewer bait stations are required for effective L. humile control making low toxicity baits a more affordable and practical method of controlling L. humile than the other two ant species. Food preference trials showed that L. humile and C. peringueyi have a high preference for sugar while A. custodiens significantly preferred tuna over other baits. However, all ant species had a preference for wet baits (25% sugar water, 25% honey, tuna & agar) as opposed to dry ones (fish meal, sorghum grit, peanut butter & dog food). This research concludes that low toxicity baits show potential in ant pest management and can offer producers with a more practical, economical and environmentally friendly method of ant control which is compatible with vineyard IPM programmes. / AFRIKAANSE OPSOMMING: Mierbesmetting wat uit die Argentynse mier Linepithema humile (Mayr), die gewone malmier Anoplolepis custodiens (F. Smith) en die wipstertmier Crematogaster peringueyi Emery bestaan, is ’n plaagprobleem wat wydverspreid in Suid-Afrikaanse wingerde voorkom. Programme vir geïntegreerde plaagbeheer (GPB) wat daarop gemik is om die wingerdwitluis Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae) – wat ’n probleem is weens die heuningdou wat dit afskei – op druiwe te beheer, moet mierbeheer insluit om sodoende die uitwerking en doeltreffendheid van die witluis se natuurlike vyande die beste te benut. As miere uitgeskakel kan word, sal dit vir die natuurlike vyande moontlik wees om die witluis sodanig te beheer dat dit onder die ekonomiese drempelvlakke (EDV) bly. Huidige strategieë om miere te beheer, is beperk en sluit gewoonlik die toediening van insekdoders in wat lank neem om in die grond af te breek, wat skadelik vir die omgewing is, waarvan die toediening arbeidsintensief is en wat die natuurlike biologiese beheer kan versteur indien dit verkeerd toegepas word. Daarom is ’n meer praktiese metode ondersoek waar miere deur die gebruik van lae toksisiteit lokase beheer word. Ondersoeke na lokaasvoorkeure en lokaasaanvaarbaarheid in die praktyk, wat daarop gemik is om te bepaal of die lokaas onderskeidelik afstootlik en smaaklik bevind word, is oor lente, somer en herfs in drie verskillende wingerde in die Kaapse wynlandstreek gedurende die 2007/08-seisoen uitgevoer. Vyf gifstowwe, bestaande uit gourmet ant bait (0.5%), boorsuur (0.5%), fiproniel (0.0001%), fenoksiekarb (0.5%) en spinosad (0.01%) wat in ’n 25%-suikeroplossing opgelos is, is getoets teenoor ’n kontrole wat uit ’n 25%-sukrose-oplossing bestaan. Al die mierspesies het gourmet ant bait bo die ander lokase verkies en aanvaar. In die laboratorium is ondersoeke gedoen om die doeltreffendheid van die lokase te bepaal deur vier insekdoders (gourmet ant bait, boorsuur en spinosad) te gebruik in konsentrasies van 0.25; 0.5; 1; 2 en 4 keer die dosis in die praktyk en fiproniel teen 0.015625; 0.03125; 0.0625; 0.125; 0.25 keer die dosis in die praktyk. Resultate het getoon dat boorsuur (2%), gourmet ant bait (2%) en fiproniel (1.0 X 10-5%) vertraagde toksisiteit getoon het vir L. humile en C. peringueyi, terwyl spinosad (0.01%) ’n vertraagde uitwerking getoon het op L. humile. Toetse om kossoekaktiwiteite in die praktyk en die voedselvoorkeure van die drie mierspesies te ondersoek, is ook gedurende die 2007/08-seisoen gedoen. Proewe oor kossoekaktiwiteite het getoon dat hierdie aktiwiteite in die wingerd by L. humile hoër is in verhouding met A. custodiens en C. peringueyi. Dit beteken dat minder lokaasstasies nodig is om L. humile doeltreffend te beheer en lei daartoe dat lae toksisteit lokaas ’n beter manier is om L. humile te beheer as die ander twee mierspesies. Proewe oor voedselvoorkeure het aangedui dat L. humile en C. peringueyi ’n groot voorkeur toon vir suiker, terwyl A. custodiens ’n duidelike voorkeur vir tuna het. Alle mierspesies het egter ’n voorkeur vir nat lokaas (25% suikerwater, 25% heuning, tuna en agar), eerder as droë lokaas (vismeel, sorghumgruis, grondboontjiebotter en hondekos) getoon. Uit hierdie navorsing word afgelei dat lae toksisteit lokaas potensiaal toon in mierbeheer en dat dit produsente ’n meer praktiese, ekonomiese en omgewingsvriendelike metode van mierbeheer kan bied wat met GPB-programme in die wingerd versoenbaar is.

PDMS has not only the characteristics of good chemical and physical properties,flexible, low surface free energy, and good optically transparent, also has the advantages of low cost, low toxicity and environmental protection. In this study we use PDMS to fabricate liquid crystal device with different micro-confined structures. We had a discussion about liquid crystal in the micro-confined structure through optical textures and measured electro-optical characteristics on our device. Through the experiment we found the liquid crystal device with micro-confined structure had not only the characteristics of wide viewing angle and contrast ratio has been up to standard in normal display. And it also demonstrated the capability in flexible liquid crystal display.

Content: The toxicity and unpleasant smell of hydrogen sulphide (H2S) gas is an issue for the leather industry that has been contained rather than eliminated in tannery practice. Completely eliminating H2S from tanneries while maintaining practical and economically feasible processing is still a big challenge to be addressed. Significant progress has, though, been made by introducing robust and reliable low sulphide unhairing systems based on selective soaking and specific enzymatic liming auxiliaries. These systems allow the reduction of sodium sulphide offers from the typical 2.5% to 1% of pelt weight. These lower levels reduce the amount of hydrogen sulphide gas released into the environment from the liming float, as well as the amount of sulphide that is carried over in the hide to subsequent processing steps. Overall, the H2S problem is not eliminated, but significantly reduced with this technology. In a further evolution of the technology, organic thio compounds can be used to fully or partially replace the already low levels of sulphide required, and thus allow to operate with offers well below 1%, or even completely without inorganic sulphide. Due to their reductive power, the organic thio compounds react with keratin in a similar way as inorganic sulphides, but they are oxidized much faster, which is advantageous for the effluent load. Alternatively to, or in combination with organic thio compounds, H2S scavengers can be used to reduce or eliminate hyrogen sulphide released from liming floats. Different types of scavengers are available, but the selection is limited for technical and economic reasons. The paper on hand shows how the release of hydrogen sulphide from beamhouse operations can be effectively controlled by a combination of technologies involving more effective unhairing with lower amounts of reductive agents, replacement of inorganic sulphide by organic thio compound and the use of H2S scavengers. Take-Away: - The release of toxic H2S from Beamhouse operations can be effectively controlled using modern unhairing technologies allowing to use no or low sulphide. - These modern unhairing systems are based on a combination of selective soaking, unhairing assisted by enzymes with specific activity and the use of organic thio compounds.

Macromolecular biomaterials often require covalent crosslinking to achieve adequate stability and mechanical strength for their given application. However, the use of auxiliary chemicals may be associated with long-term toxicity in the body. Oppositely-charged polyelectrolytes (PEs) have the advantage that they can self-crosslink electrostatically and those derived from marine organisms are an inexpensive alternative to glycosaminoglycans present in the extracellular matrix of human tissues. A range of different combinations of PEs and preparation conditions have been reported in the literature. However, although there has been some work on complex formation between chitosan (CS) and carrageenan (CRG), much of the work undertaken has ignored the effect of pH on the consequent physicochemical properties of self-crosslinked polyelectrolyte complex (PEC) gels, films and scaffolds. Chitosan is a positively-charged polysaccharide with NH3+ side groups derived from shrimp shells and, carrageenan is a negatively-charged polysaccharide with OSO3- side groups derived from red seaweed. These abundant polysaccharides possess advantageous properties such as biodegradability and low toxicity. However, at present, there is no clear consensus on the cell binding properties of CS and CRG or CS-CRG PEC materials. The aim of this study was to explore the properties of crosslinker-free PEC gels, solvent-cast PEC films and freeze-dried PEC scaffolds based on CS and CRG precursors for medical applications. The objective was to characterise the effect of pH of the production conditions on the physicochemical and biological properties of CS-CRG PECs. Experimental work focused on the interaction between PEs, the composition of PECs, the rheological properties of PEC gels and the mechanical properties of PEC films and scaffolds. In addition, cell and protein attachment to the PEC films was assessed to determine their interactions in a biological environment. For biomedical applications, these materials should ideally be stable when produced such that they can be processed to form either a film or a scaffold and have mechanical properties comparable to those of collagenous soft tissues. FTIR was used to confirm PEC formation. Zeta potential measurements indicated that the PECs produced at pH 2-6 had a high strength of electrostatic interaction with the highest occurring at pH 4-5. This resulted in stronger intra-crosslinking in the PEC gels which led to the formation of higher yield, solid content, viscosity and fibre content in PEC gels. The weaker interaction at pH 7-12 resulted in higher levels of CS incorporated into the complex and the formation of inter-crosslinking through entanglements between PEC units. This resulted in the production of strong and stiff PEC films and scaffolds appropriate for soft tissue implants. The PECs prepared at pH 7.4 and 9 also exhibited low swelling and mass loss, which was thought to be due to the high CS content and entanglements. From the range of samples tested, the PECs produced at pH 7.4 appeared to show the optimum combination of yield, stability and homogeneity for soft tissue implants. Biological studies were performed on CS, CRG and PECs prepared at pH 3, 5, 7.4 and 9. All of the PE and PEC films were found to be non-cytotoxic. When the response of three different cell types and a high binding affinity protein (tropoelastin) was evaluated; it was found that the CS-CRG PEC films displayed anti-adhesive properties. Based on these experimental observations and previous studies, a mechanistic model of the anti-adhesive behaviour of PEC surfaces was proposed. It was therefore concluded that the CS-CRG PECs produced might be suitable for non-biofouling applications.