ŠIMKOVÁ, D. Reproduction of an African elephant (Loxodonta africana) in a Zoo Zlín – Lešná. Mendel University in Brno, 2019. Diploma thesis. The thesis analyzes the issue of reproduction of critically endangered species of African elephant. The work is focused on monitoring of animal weight and blood test results during the reproductive cycle. The first part describes the general characteristics of the reproduction cycle of the African elephant, the issue of pregnancy and childbirth, and the female reproductive system. In the second part, there are given data from three particular elephants from the Zoo in Zlín. The data are processed into graphs and these are then commented on and compared with the conclusions of already conducted studies and scientific papers. At the end, all the knowledge and forecast of the African elephant reproduction in the Zoo - Lešná in the future are evaluated. All the results show that females are fully capable of reproduction and there is no need to make any changes in the breeding of these animals.

A research report submitted to the Faculty of Science, University of the Witwatersrand, in partial fulfilment of the requirements for the degree of Masters of Science (Environmental Sciences). September 2015. / African elephants (Loxodonta africana) are still being poached and hunted and this could potentially affect their movement. As a keystone species, crucial to maintaining ecosystem function and therefore food webs, it is important to understand the effects hunting may have on their movements. This study was undertaken in the Associated Private Nature Reserves adjacent to the Kruger National Park, where elephants are hunted. I looked at changes in daily displacement distance and distance from hunting and natural mortality events to determine if elephants were affected by hunting events, up to what distance, and to determine whether elephants reacted in the same way to hunting and natural mortality events. I found that elephants are only affected by hunting events when within 1 km of the event, which is consistent with the literature. When considering natural mortality events, they actually diverted movements towards the event location, which is also consistent with literature. These results, however, are based on a very small sample size. In addition, the time of day of the events was not recorded by the wardens of the reserve, and this provided a further limitation for the data analysis and interpretation.

Philosophiae Doctor - PhD / The survival of free-ranging (in situ) African elephant and Southern white rhinoceros populations are currently being challenged on a daily basis in Africa. Reproductive health is considered a vital component of species conservation. Conservation of the last mega land mammals may ultimately require intervention by breeding management or combined with assisted reproductive technologies (ART). There is a strong case for gathering baseline information, both physiological and biological, of any species, as opportunities arise. During this study a total number of 21 ejaculates collected over two seasons from 12 free-ranging African elephant bulls were characterised, as well as 10 ejaculates collected from 10 free-ranging Southern white rhinoceros bulls from two populations. Ejaculates were collected from adult bulls by means of electroejaculation under anaesthesia. Routine semen analysis was combined with Computer-aided sperm analysis (CASA), Computer-aided sperm morphology analysis (CASMA), Transmission electron microscopy (TEM) and Genomics as diagnostic tools. Additionally, sperm functionality within different media was investigated and sperm subpopulation classification according to the motion pattern displayed. The results presented is based on the evaluation and classification of ≈ 45 000 individual African elephant spermatozoa and ≈ 18 000 individual Southern white rhinoceros spermatozoa. The average elephant ejaculate contained a total number of 47 x 10⁹ spermatozoa (volume of 56 ± 38mL x concentration of 818 ± 750 x 10⁶/mL) that recorded a total motility of 81 ± 29% of which 62 ± 26% were progressively motile. CASA recorded velocities for curvilinear velocity (VCL 241 ± 58μm/s), straight-line velocity (VSL 173 ± 181μm/s) and average path velocity (VAP 201 ± 54μm/s), and kinematics at straightness of track (STR 86 ± 85%), linearity of track (LIN 67 ± 16%), amplitude of lateral head displacement (ALH 4 ± 0.75μm) and beat cross frequency (BCF 21 ± 3Hz). Structural analysis revealed 68 ± 11% of the spermatozoa were viable (intact plasma membrane) and 77 ± 11% maintained acrosome integrity. Ejaculates contained 55 ± 14% morphologically normal spermatozoa, CASMA measured sperm head lengths at 6.83 ± 0.26μm and width 3.32 ± 0.18μm (total head area of 20.17 ± 1.96μm²) of which 38.95 ± 0.92% is covered by an acrosomal cap. The average rhinoceros ejaculate contained a total number of 1.1 x 10⁹ spermatozoa (volume of 24 ± 24mL x concentration of 83 ± 96 x 10⁶/mL) that recorded a total motility at 82 ± 8% of which 28 ± 23% were progressively motile. CASA recorded velocities for VCL (85 ± 29μm/s), VSL (44 ± 25μm/s) and VAP (69 ± 30μm/s, and kinematics at STR (63 ± 14%), LIN (51 ± 16%), ALH (2 ± 0.16μm) and BCF (16 ± 6Hz). Structural analysis revealed 73 ± 10% of the spermatozoa were viable (intact plasma membrane) and 76 ± 4% maintained acrosome integrity. Ejaculates contained 62 ± 14% morphologically normal spermatozoa, CASMA measured sperm head lengths at 5.5 ± 0.17μm and width 2.9 ± 0.19μm (total head area of 14.8 ± 1.43μm²) of which 36.3 ± 0.59% is covered by an acrosomal cap. Based on a Boolean argument and CASA data exploration it was possible to derive elephant and rhinoceros CASA cut-off criteria to sort between activated and hyperactivated motile spermatozoa. For the genomic component of this study, the CatSper1 (Loxodonta africana) gene was identified,sequenced and verified in a free-ranging (natural) African elephant population. Multivariate analysis(MVA) was applied to examine the associations between the semen and sperm parameters and the traits they accounted for in this study. Our understanding of wildlife reproductive sciences can substantially progress as the analytical techniques applied and the combination thereof is expanded. This investigation presents a new set of comprehensive semen and sperm threshold values for future investigations.

The aim of the study was to determine the consequences of elephant (Loxodonta africana) impact on selected nesting sites of avian fauna and other species in the Associated Private Nature Reserves. The study also aimed at answering key questions on how the architecture of trees influence nest site suitability and what landscape features affect nest site location. Furthermore the type of impact that elephants have on specific nesting sites was determined and how this would affect the short term persistence of these trees. The facillitatory role of elephants was examined by looking at the type of impact that produces gum exudants as well as what gum is selected for by primates and whether primary branch breaking may lead to the creation of nesting sites for species such as the southern ground hornbill (Bucorvus leadbeateri). / Environmental Sciences / M. Sc. (Environmental Management)

Compromised welfare and wellbeing of elephants (Loxodonta africana and Elephas maximus) in captive facilities are significant and global problems. The period between birth and two years old is crucial for calf survival and social and environmental learning. Behaviour and developmental processes among captive elephant calves in these first years were compared with those seen in wild calves. Wild elephants calves develop within a complex, varied social context and provide one reference for normal patterns of development. Such comparisons enable insights into welfare at captive facilities. Eleven captive elephant calves born at three UK facilities were studied from birth to 18 months (AsianN=6; AfricanN=5). Older calves (AsianN=2; AfricanN=2) were also sampled up to 3.5 years; making a total of 15 calves studied from 2009 to 2014. Due to the small sample size, the 11 younger calves were also discussed as individual case studies. By 2017, only two of these case study calves were both alive and not orphaned. Three additional calves (AsianN=1; AfricanN=2) died on their day of birth and were not sampled. This small sample highlights the ongoing lack of self-sustaining populations of captive elephants. This thesis collated systematic behavioural observations on captive calves across 373 days (483.5hrs). Calf maintenance activities (feeding, resting, moving), associations with mother and others, interactions and calf play were compared with behavioural observations of wild AsianN=101 (74hrs, Uda Walawe, Sri Lanka) and wild AfricanN=130 (252hrs, Amboseli, Kenya) calves from ~birth to five yrs. Mothers’ (captive: AsianN=4; AfricanN=4; wild: AsianN=90; AfricanN=105) activities were also recorded to explore synchrony with calves. Captive calves raised by their mothers had similar activity budgets to those seen in the wild. Expected age-related declines in suckling were found in captivity. However, captive calves were more independent than wild calves for their age in distance from mother and spent significantly more time in play. A Decision Tree for whether to breed elephants in captivity was developed; benefits that a calf potentially brings to companions, e.g. multi-generational matrilineal groups, enabling social bonding and reducing abnormal behaviours, were considered against space required for families to grow and divide naturally over time, as well as ensuring that captive-bred males are socially sustained. It was recommended that facilities invest in future enclosure/housing designs which permit: free-access to other elephants; 24hr trickle feeding; juvenile males allowed to stay with their maternal group for longer, encouraging learning opportunities and further retaining age-structure/composition. Conversely, facilities unwilling to house a male or provide appropriate group size/composition are recommended to cease breeding.

The aim of the study was to determine the consequences of elephant (Loxodonta africana) impact on selected nesting sites of avian fauna and other species in the Associated Private Nature Reserves. The study also aimed at answering key questions on how the architecture of trees influence nest site suitability and what landscape features affect nest site location. Furthermore the type of impact that elephants have on specific nesting sites was determined and how this would affect the short term persistence of these trees. The facillitatory role of elephants was examined by looking at the type of impact that produces gum exudants as well as what gum is selected for by primates and whether primary branch breaking may lead to the creation of nesting sites for species such as the southern ground hornbill (Bucorvus leadbeateri). / Environmental Sciences / M. Sc. (Environmental Management)