Plastid division, sustained by the equilibrium expression and coordination of plastid division genes is vital for the maintenance of plastid populations in dividing plant cells. Macrochloroplasts (MCP), the occurrence of one or a few chloroplasts per cell is due to the imbalance in the expression of plastid division genes. Because of the MCP size and number it was proposed that they may provide better targets for the plastid transformation than the normal (WT) chloroplasts and result in better plastid transformation frequencies. The objective of this research was to produce transgenic plants containing macrochloroplasts by nuclear transformation and then to use these plants as a model for the development of plastid transformation of crop species. By using AtFtsZ1-1 and AtMinD1 as query sequences in the TIGR (U.S.A) and ASTRA (Australia) Brassica oleracea EST databases, this project resulted in the isolation of cauliflower FtsZ1-1 (EU684588) and MinD (EU684589) genes. In addition, AtFtsZ1-1 was used as a control gene for comparison to the cauliflower FtsZ1-1. Binary vectors were constructed to express these genes in tobacco and cauliflower either by Agrobacterium tumefaciens-mediated or PEG-mediated transformation methods. Transgenic tobacco and cauliflower plants with abnormal chloroplasts (MCP, minichloroplasts, honeycomb or doughnut shaped chloroplasts, uneven surface membrane chloroplasts) were developed. Furthermore, the transgenic tobacco and cauliflower plants were examined by PCR, RT-PCR and Southern blotting. In addition, th ese plants were also analysed for the different abnormal chloroplast phenotypes by fluorescence microscopy. This project also generated the first plastid transformants from macrochloroplast bearing tobacco plants via biolistics. After one round of regeneration homoplasmic plastid transformants were obtained from both WT chloroplast and MCP tobacco plants. The homoplasmic nature of plastid transformants were confirmed by PCR and Southern blotting. Plastid expression of GFP in WT and MCP was confirmed by fluorescence/confocal microscopy and western blot analysis. This project showed for the first time the characterisation of cauliflower FtsZ1-1 and MinD plastid division genes in homologous and heterologous systems (cauliflower and tobacco). Moreover, obtaining homoplasmic plastid transformant shoots from one round of regeneration from the MCP containing tobacco plants is reported for the first time in this study. In addition this study explored the effect of transgene expression level on the chloroplast abnormality, highlighting the importance of analysing transgenic tobacco and cauliflower plants at the protein lev el specifically with regard to plastid division genes. The maintenance of MCP phenotype in the regenerated shoots and the requirement of standardisation of MCP containing plants via biolistics for increasing the plastid transformation frequency were also examined.

It is estimated that over 200,000 Australians have problem or chronic wounds at any one time (Australian Wound Management Association, 2008). Over the past 4 decades while there has been significant advancement in wound care, a high proportion of wounds become chronic. Despite the availability of wound care resources and specialist services, there remains an inconsistency in the management of chronic wounds that impacts both on the quality of life of individuals with chronic wounds and the health care budget (Harding 2002). Using a Grounded theory approach, the aim of this study was to explore and describe the impact of living with a chronic wound and findings indicate that individuals living with a chronic wound are receiving conflicting or inappropriate advice and care. Individuals living with a chronic wound experience a life of uncertainty related to the struggle to endure a wounded body and the layers of professional care they receive. When they are provided with conflicting or inappropriate advice and treatment, inconsistencies of care and poor coordination of care, layers of unnecessary burden are added to their experience. The uncertainty and dissonance individuals are faced with, leads them to question their care, themselves and the expertise and professionalism of their treating health professionals. As a result, they experienced a loss of respect and trust for their treating health professionals and a loss of confidence in their care. Chronic wounds impose of individuals, an intense burden of physical suffering, cause major disruption to the normality of their lives, and often entail a constant personal struggle to secure appropriate care and understanding from their treating health professionals. In order to enable individuals living with chronic wounds to develop appropriate coping strategies, it is essential that health professionals: understand the burden of suffering associated with living with a chronic wound; ensure that they develop and maintain a high level of knowledge with regards to contemporary wound care practices; ensure that their clientele are provided with high quality care information that is based on the best available evidence; ensure continuity of care; and foster quality professional-client relationships that negates the need for individuals to have to constantly question their care.