Made available in DSpace on 2014-12-17T14:13:21Z (GMT). No. of bitstreams: 1 PatriciaFM_tese.pdf: 303057 bytes, checksum: d3d1e5a21de3ce58bcfe65ef800c8734 (MD5) Previous issue date: 2008-05-13 / The uses of radiobiocomplexes labeled with technetium-99m contributed to health science advances. Stannous chloride (SnCl2) has been used as a reducing agent for the labeling process. Cytotoxic and genotoxic effect of the SnCl2 have been described in several studies and with this experimental models alterations in molecular and cellular level can be evaluated. In the last years the physicals therapists acquired new devices which emits electromagnetic radiation such us Extremely Low Frequency Pulsated Electromagnetic Fields (E.L.F. P.E.M.F.), radiofrequency, Intense Pulsed Light (I.P.L.) and others which emits sonic waves such us Biorresonance. Scientific evidence of the effects and dosage is important to protect public health and to reach exposition levels that result in significant biological effects. The aim of this project is to verify the effects of these physical agents in plasmid DNA and E. coli AB1157 cultures in presence or absence of SnCl2 and the effects in blood constituents labeled with technetium-99m. Wistar rats blood was exposed to the cited sources and the labelling of blood constituents with 99mTc was carried through. Cultures of E. coli AB1157 and plasmidial samples DNA had been also exposed the physical agents. The results suggest that these agents are capable of altering neither the survival of E. coli cells or plasmid DNA electrophoresis mobility. The multidiscipline character was clearly in this study due the interaction between Nuclear Medicine department of the UERJ and the Laboratory of Physical Agents of the Maimonides University in Argentina until the union between the teacher (biomedical and physiotherapist) and student (physiotherapist), besides collaborators of the area of Physics and Biology, promoting new ideas and perspectives and also adding the knowledge of different areas and origins / O uso de radiobiocomplexos marcados com 99mtecn?cio tem contribu?do para avan?os em Ci?ncias da Sa?de. Na marca??o desses radiobiocomplexos utiliza-se o redutor cloreto estanoso (SnCl2). Efeitos citot?xicos e genot?xicos do SnCl2 t?m sido descritos. Atrav?s de modelos experimentais em n?vel molecular, celular e sist?mico estas altera??es podem ser avaliadas. Entre os novos agentes f?sicos sugeridos para a pr?tica da Fisioterapia Dermato-Funcional, h? os que s?o de natureza eletromagn?tica (campos eletromagn?ticos pulsados, a radiofreq??ncia e a luz intensa pulsada) e os de natureza mec?nica (ondas s?nicas). A literatura a respeito dos agentes f?sicos gera d?vidas quanto aos seus efeitos. Seu uso correto ? importante n?o s? para proteger a sa?de p?blica, mas tamb?m para justificar n?veis de exposi??o que resultem em efeitos biol?gicos significativos. Dessa forma, o objetivo desse estudo ? verificar os poss?veis efeitos destes agentes f?sicos em plasm?dios bacterianos e em culturas bacterianas na presen?a e na aus?ncia de cloreto estanoso e na marca??o de elementos sang??neos com o 99mtecn?cio. Sangue de ratos Wistar foi exposto ?s fontes citadas e a marca??o de c?lulas sangu?neas com 99mtecn?cio foi realizada. Culturas de E. coli AB1157 e amostras DNA plasmidial tamb?m foram expostas aos agentes f?sicos. As consequ?ncias biol?gicas associadas a esses agentes n?o foram capazes de interferir em algumas propriedades dos constituintes sangu?neos, nem na sobreviv?ncia de E. coli ou na mobilidade eletrofor?tica do DNA plasmidial. O car?ter multidisciplinar ficou claro neste estudo desde a intera??o entre o Setor de Medicina Nuclear da UERJ e o Laborat?rio de Agentes F?sicos da Universidade Maimonides na Argentina at? a uni?o entre um orientador (biom?dico e fisioterapeuta) e uma orientanda (fisioterapeuta), contando com colaboradores da ?rea de F?sica e Biologia, possibilitando novas id?ias e perspectivas, agregando o conhecimento de diferentes ?reas