Wong, Ying Chun. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 73-78). / Abstracts in English and Chinese. / Acknowledgements --- p.I / Abstract --- p.II / 摘要 --- p.IV / List of Tables --- p.VI / List of Figures --- p.VII / List of Abbreviations --- p.VIII / Chapter Chapter 1 --- Introduction --- p.3 / Chapter 1.1 --- Chemical Constituents of Rhodiola Genus --- p.5 / Chapter 1.1.1 --- Phenylethyl Derivatives --- p.5 / Chapter 1.1.2 --- Phenylpropanoids --- p.8 / Chapter 1.1.3 --- Phenolic Derivatives --- p.11 / Chapter 1.1.4 --- Flavonoids --- p.14 / Chapter 1.1.4.1 --- Flavone and Flavone Glycosides --- p.15 / Chapter 1.1.4.2 --- Flavonols and Their Glycosides --- p.17 / Chapter 1.1.4.3 --- Flavan-3-ol Derivatives --- p.23 / Chapter 1.1.5 --- Monoterpenoids --- p.26 / Chapter 1.1.6 --- Triterpenes --- p.30 / Chapter 1.1.7 --- Miscellaneous Compounds --- p.32 / Chapter 1.2 --- Biological Activities of Rhodiola Genus --- p.33 / Chapter 1.2.1 --- Anti-oxidative Effect --- p.34 / Chapter 1.2.1.1 --- Protective Effect on Ischemia and Reperfusion --- p.34 / Chapter 1.2.1.2 --- Anti-Aging Effect --- p.35 / Chapter 1.2.2 --- Learning and Memory --- p.36 / Chapter 1.2.3. --- Immune Response --- p.37 / Chapter 1.2.4 --- Anti-cancer Effect --- p.38 / Chapter 1.3 --- Objective --- p.39 / Chapter Chapter 2 --- Experimental --- p.40 / Chapter 2.1 --- General Experimental Procedures --- p.40 / Chapter 2.2 --- Plant Materials --- p.40 / Chapter 2.3 --- Extraction and Isolation --- p.41 / Chapter 2.3.1 --- Isolation and Purification of the Ethyl Acetate (E.A.) Fraction --- p.41 / Chapter 2.3.2 --- Isolation and Purification of the Butanol Fraction --- p.44 / Chapter 2.4 --- Characterization of the Isolated Compounds --- p.46 / Chapter 2.4.1 --- β-Sitosterol (1) --- p.46 / Chapter 2.4.2 --- Tyrosol (2) --- p.46 / Chapter 2.4.3 --- trans-Hydroxycinnamic acid (3) --- p.47 / Chapter 2.4.4 --- Geranyl-β-glucopyranoside (4) --- p.47 / Chapter 2.4.5 --- Neryl-β-glucopyranoside (5) --- p.48 / Chapter 2.4.6 --- Hexyl β-Glucopyranoside (6) --- p.48 / Chapter 2.4.7 --- Gallic Acid (7) --- p.49 / Chapter 2.4.8 --- Epigallocatechin-3-Gallate (8) --- p.49 / Chapter 2.4.9 --- Rhodiolgin (9) --- p.50 / Chapter 2.4.10 --- lsolariciresinol-9-β-Glucopyranoside (10) --- p.51 / Chapter 2.4.11 --- Rhodiooctanoside (11) --- p.52 / Chapter 2.4.12 --- Sacranoside B (12) --- p.52 / Chapter Chapter 3 --- Results and Discussion --- p.53 / Chapter 3.1 --- Structural Determination of the Isolated Compounds --- p.53 / Chapter 3.1.1 --- Identification of β-sitosterol (1) --- p.53 / Chapter 3.1.2 --- Identification of Tyrosol (2) --- p.54 / Chapter 3.1.3 --- Identification of trans-Hydroxycinnamic Acid (3) --- p.55 / Chapter 3.1.4 --- Identification of Geranyl-jS-glucopyranoside (4) --- p.56 / Chapter 3.1.5 --- Identification of Neryl-β-glucopyranoside (5) --- p.58 / Chapter 3.1.6 --- Identification of Hexyl β-Glucopyranoside (6) --- p.59 / Chapter 3.1.7 --- Identification of Gallic Acid (7) --- p.60 / Chapter 3.1.8 --- Identification of (-)-Epigallocatechin 3-Gallate (8) --- p.61 / Chapter 3.1.9 --- Identification of Rhodiolgin (9) --- p.63 / Chapter 3.1.10 --- Identification of lsolariciresinol-9-β-glucopyranoside (10) --- p.65 / Chapter 3.1.11 --- Identification of Rhodiooctanoside (11) --- p.67 / Chapter 3.1.12 --- Identification of Sacranoside B (12) --- p.69 / Chapter Chapter 4 --- Conclusion --- p.70 / References --- p.73

Thesis (M.Tech.: Homoeopathy)-Dept. of Homoeopathy, Durban University of Technology, 2007. xi, 238 leaves / The first objective of this study was to determine the symptomatology that the plant remedy Peucedanum galbanum 30CH, produced in healthy individuals, so that it could be prescribed according to the Law of Similars, as required by homoeopathy. The second objective was to analyse this symptomatology in relation to the doctrine of signatures. It was hypothesised that Peucedanum galbanum 30CH would produce symptomatology in healthy individuals which would correlate to the doctrine of signatures of the plant.

Thesis (M.Tech.: Homoeopathy)-Dept. of Homoeopathy Durban University of Technology, 2007. 250 leaves / This research study was conducted to elucidate the total symptomatology that the plant Peucedanum galbanum in the thirtieth centesimal potency would produce on healthy individuals so that it may be prescribed according to the Law of Similiars. A further aim of the investigation was to compare this remedy to other remedies yielding the highest numerical values and total number of rubrics on repertorisation of the proving symptoms. Wagner (2007), in a concurrent study, analysed the proving results of Peucedanum galbanum in relation to the doctrine of signatures. It was hypothesised that Peucedanum galbanum 30CH would produce recognizable signs and symptoms in healthy provers, and that the comparative study of this remedy would highlight the differences and similarities between remedy symptoms and thus confusion as to indication is eliminated, plus a fuller understanding of the remedy and its relationship to other remedies is gained.

Mini-dissertation submitted in partial compliance with the requirements for the Master’s Degree in Technology: Homoeopathy, Durban University of Technology, 2011. / Introduction The purpose of this study was to determine the therapeutic potential of Curcuma longa 30CH when administered to healthy individuals, thus revealing the materia medica of the substance. It was also the aim of this study to compare the existing therapeutic indications of the substance to the proving symptomatology. Methodology The proving took the form of a double-blind placebo controlled study and was conducted by two Master’s in Technology: Homoeopathy students using 30 healthy subjects. Twenty four provers were given the active medication and six provers were given the placebo. The remedy was manufactured according to the German Homoeopathic Pharmacopoeia in 30CH potency. The proving ran for a period of six weeks. Results The symptoms extracted from the proving were placed in different sections according to the repertory and was compared to the Ayurvedic and Phytotherapeutic indications of Curcuma longa. There were 202 symptoms produced as a result of the remedy, 141 rubrics were formulated using these symptoms. The largest number of rubrics i ii was allocated to the mind, head and dreams section of the repertory, other smaller sections of prominence included the eye, ear, nose and throat sections. A wealth of information was gained once the comparison was made between Curcuma longa 30CH and the Phytotherapeutic and Ayurvedic indications of use. Similarities between the materia medica of Curcuma longa and the Phytotherapeutic indications of use were found to exist with respect to sections such as eye, nose, face, stomach, stool, respiration, back, extremities, skin and generals. Conclusion The administration of Curcuma longa 30C to healthy provers according to the methodological protocol of this study resulted in the production of a variety of defined proving symptoms which comprise the materia medica thereof (first objective of the study). The subsequent comparison of the proving symptoms with the existing indications of Turmeric as an Ayurvedic and Phytotherapeutic medicine (second objective of the study) revealed clear correlations in a variety of defined areas.

Mini-dissertation submitted in partial compliance with the requirements for the Master’s Degree in Technology: Homoeopathy in the Department of Homoeopathy, Durban University of Technology, 2011. / The aim of this study was to determine the effect that Curcuma longa 30CH would have on healthy individuals, and record the particular signs and symptoms produced. These signs and symptoms determine the therapeutic indications of this remedy, so that it may be prescribed according to the homoeopathic Law of Similars. The second aim of this study was to analyse the symptomatology of Curcuma longa 30CH in relation to a Doctrine of Signatures analysis of the Curcuma longa plant, in order to facilitate a more comprehensive understanding of the materia medica of this substance. Design The homoeopathic proving of Curcuma longa in 30CH potency took the form of a double blind, randomized, placebo controlled trial. Thirty healthy provers were selected on the basis of them meeting with the necessary inclusion criteria (Appendix A). The provers were randomly divided into 2 groups, of which 20% (6 of the 30 provers) formed the placebo group and received non-medicated powders, and the remaining 80% (24 of the 30 provers) received medicated powders (verum). The 2 groups were not aware of the nature of the substance that they were proving or the potency used. The provers recorded their mental, physical and emotional states over a period of a week prior to taking the remedy in order to establish a baseline for comparison after the administration of the remedy. Both verum and placebo were dispensed in the form of 6 powders. Each powder was taken sublingually 3 times daily for 2 days or until the prover experienced the onset of any symptoms. Each prover kept a journal and recorded their proving signs and symptoms daily after administration of the remedy or the placebo. The data was collected and extracted from these journals and then assessed by the researcher for suitability to be included in the materia medica of Curcuma longa. All data gathered from the case histories (Appendix C), physical examinations and group discussions were also considered for inclusion. Results A variety of mental, emotional and physical symptoms were produced and included in the materia medica of Curcuma longa. There were a total number of 202 symptoms that were produced as a result of the remedy, which resulted in the formulation of 141 rubrics. The main mental and emotional symptoms that surfaced during the proving were depression, a deep sadness, changeability of moods, courage/confidence, relaxed/ calm and less anger, agility, increased concentration, and vivid dreams. The physical symptoms noted were diarrhea, change in energy levels (too much or too little energy), burning sensations, headaches, heart palpitations and increased breathing rates. The symptoms that came about during the proving clearly showed correlation and association with the nature and description of the Turmeric plant, this is in keeping with findings of previous provings (Pistorius, 2006; Webster, 2002; Speckmeier, 2008 & Pather, 2009), furthermore as suggested by Richardson-Boedler (1999:173) the Doctrine of Signatures analysis of the Turmeric plant facilitated in the interpretation of the proving symptoms and thus the materia medica of the remedy.

Submitted in fulfillment of the requirements of the Degree of Doctor of Technology: Homoeopathy, Durban University of Technology, 2011. / Aim This study sought to appraise homoeopathic proving methodology as a bridge between the indigenous and rationalist-scientific understandings of medicinal plants through a detailed exploration of the relationships existing between data derived from respective paradigmatic explorations of a single African traditional medicinal plant, Strychnos henningsii [Red bitterberry]. Methods The data derived from the implementation of a triple-blind, placebo-controlled homoeopathic proving methodology, on 32 healthy human subjects (50 percent placebo), using the bark of Strychnos henningsii in the 30CH potency, were evaluated for internal consistency and coherence, and subsequently compared to data derived from a phytochemical analysis of the crude bark sample, and translated data derived from semi-structured mothertongue interviews of eight Zulu traditional healers. The proving data took the form of subjective journal data and the results of four objective blood measures of erythrocyte sedimentation rate (ESR), redand white blood cell indices, and liver functions. The subjective data were evaluated in terms of defined inclusion criteria and presented in standard materia medica and repertory formats, and tabulations of objective data were subjected to independent statistical analysis, using repeated-measures ANOVA and profile plots. The crude bark sample was analysed in terms of the presence of strychnine and other indole alkaloids, using highperformance liquid chromatography-mass spectrometry, and interview data related to the indigenous understanding and application of Strychnos henningsii within the traditional African medical paradigm, were audiovisually recorded, collaboratively translated, and independently verified. ABSTRACT iii Qualitative data processing and analysis was effected with the aid of NVivo® software, and a range of comparative analyses were effected with the aid of Radar® homoeopathic software, materia medica references and the Mappa Mundi elemental theory model. Results The proving yielded 581 subjective symptoms, covering a broad range of physical and mental disease manifestations, and nine statistically-significant treatment effects within the objective data set. These included elevation of ESR and changes in two red blood cell indices, four white blood cell indices and two liver function indices. The two proving data sets were demonstrated to show high levels of correlation, although these correlations were not demonstrable for all provers. The phytochemical analysis confirmed the presence of between two and five strychnine-related compounds (excluding strychnine itself), and the field interview data served to confirm all except two documented traditions of use, as well as identifying a number of novel indications and application of Strychnos henningsii bark. The comparative analyses demonstrated the integrity of homoeopathic proving methodology as a mode of scientific investigation, and significant and widespread overlaps of proving symptomatology with both the pharmacology and toxicology of strychnine, and the physical and metaphysical understanding and application within the traditional African medical paradigm. Conclusions Homoeopathic proving methodology was discussed in terms of the evident degree of overlap with the indigenous and rationalist-scientific paradigms, and the incomplete nature of the homoeopathic ‘totality’. A number of recommendations were made for future cross-paradigmatic research. / Durban University of Technology. Postgraduate Development and Support Directorate.

Submitted in fulfillment of the Master’s Degree in Homoeopathy, Durban University of Technology Durban, 2014. / The purpose of this research study was to determine any therapeutic significance of Malus domestica (domestic apple) in the potentised, homoeopathic form and to contribute this information to the body of the homoeopathic materia medica. It was further hypothesised that some proving symptoms experienced by the provers during the research study would show a resemblance to unique characteristics of the plant in terms of its natural appearance and cultural references. This involved a detailed doctrine of signatures analysis of Malus domestica based on an extensive literature review. Combining a proving research study with comparative analysis to the doctrine of signatures helped to clarify and verify the remedy’s potential therapeutic value. Methodology This homoeopathic proving was carried out in the form of a double blind placebo controlled study of Malus domestica 30CH with a total of 30 provers. The prover sample was randomly divided into two groups: 24 provers (80%) into the verum group and the remaining six provers (20%) into the placebo group. The provers were unaware of either the proving substance or the potency used. Participants were required to record their mental, emotional and physical status’ one week prior to administration of the proving powders as a form of control for comparison of symptoms post administration of the proving remedy. Thorough physical examinations and case histories of each prover were taken prior to and after the proving period. Provers ingested one powder three times a day for three days and recorded their symptoms daily in a journal. The duration of the proving period spanned five weeks. During this period researchers were in constant contact with all participants. Once the proving period was complete, all journals were gathered and the information therein translated into materia medica and repertory format so as to develop the remedy picture of Malus domestica 30CH. A comparison between the symptomatology produced in the provers and the doctrine of signatures was then performed. Results A wide range of symptoms were documented by the provers. The results revealed an affinity to the mental plane – the most striking being the theme of being disconnected and separated from others in the environment as well as within one’s self. Marked polarities were depicted as follows: Anger/irritability vs. calmness/tranquility; Depression/sadness vs. cheerfulness; Confusion vs. concentration/clarity of mind; Connection vs. disconnection. Physically, symptoms concerning the extremities were numerous, with provers describing symptoms of their limbs being disconnected/separated as well. Head symptoms were also numerous as provers described a wide range of headaches, as well as headaches that were associated with eye symptoms. Abdominal symptoms manifesting as cramping associated with diarrhoea and, in some cases, constipation were documented. Dizziness associated with the sensation of the head floating was common as well. Numerous themes arising from dreams were recorded by provers, with a clear affinity to family members as well as dreams of social gatherings and banquets. Provers dreamt of family members placed in precarious situations wherein the lives of their loved ones were at risk, whilst the emotion of guilt was expressed in certain dreams regarding parties and banquets. The comparison between the homoeopathic drug proving of Malus domestica 30CH and the doctrine of signatures brought many similarities to light, with most of these similarities relating to the mind, extremities and head. Conclusion As hypothesised Malus domestica 30CH produced clearly observable symptoms in healthy provers. On comparison, the proving remedy and the doctrine of signatures brought many parallels to light – the majority of which related to the mind, extremities and head. To gain a complete remedy picture of Malus domestica it is imperative that further research into the symptomatology of different homoeopathic potencies be conducted. / M

Mini-dissertation was submitted in partial compliance with the requirements for the Master’s Degree in Technology: Homoeopathy, Durban University of Technology, 2012. / It was reported by the International Diabetes Federation (IDF) Diabetes Atlas, in 2003, that a prevalence figure of 3.4% exists for the 24 million South Africans between the ages of 20 and 79, with an expected increase to 3.9% by 2025. Considering that patients with diabetes are at increased risk of cardiovascular disease, blindness, amputation and renal failure it is therefore not surprising that the costs associated with diabetes are estimated to increase worldwide. It is clear that according to the current trends in dietary and exercise practices, South Africa will be affected by the rise in obesity and subsequent diabetes mellitus. It is critical that a concerted effort involving all parties concerned be made to combat this rapidly increasing problem (Rheeder, 2006:20). AIM The purpose of this double-blind, randomized clinical trial was to compare the effectiveness of Momordica charantia homoeopathic mother tincture as compared to Momordica charantia 6CH, in the treatment of type 2 diabetes mellitus in patients on Metformin. METHODOLOGY Thirty patients were recruited and were selected for the study on the basis of inclusion and exclusion criteria. These participants were then randomly and equally divided into two groups. Each participant attended a total of four consultations with the researcher, over a two month period, at the Durban University of Technology (DUT) Homoeopathic Day Clinic. At the commencement of the first consultation, each participant received the subject information letter (Appendix A) for perusal and the informed consent form (Appendix B) to sign. Following this, the researcher took a full, detailed iv case history (Appendix F) and performed a physical examination (Appendix G) of each patient. Participants were required to have a Glycosylated haemoglobin (HbA1C) test performed following the first and fourth consultations. Participants were also required to complete daily Log Sheets (comprising self administered fasting blood glucose readings using issued Bayer Ascensia Elite Glucometers) for the entire duration of the study (8 weeks). SPSS version 18 was used to analyse the data. A p value < 0.05 was considered as statistically significant. The time effect was assessed for intra-group comparison whereas the time x group treatment effect was assessed for inter-group comparison. Means were calculated for both fasting blood glucose and glycosylated haemoglobin for the two respective groups and tabulated in order to describe the data obtained (Descriptive statistics). RESULTS Both groups reflected a statistically non significant decrease in fasting blood glucose levels with no significant differences between the two groups when comparing reduction in fasting blood glucose levels. Group 1 (Momordica charantia homoeopathic mother tincture) reflected a non significant increase in glycosylated haemoglobin (HbA1C) levels while Group 2 (Momordica charantia 6CH) reflected a statistically significant increase over time in HbA1C levels. There were no significant differences between the two groups when comparing reduction in HbA1C levels. / M

Submitted in fulfillment of the requirements for the degree of Master of Technology : Homeopathy, Durban University of Technology, Durban, South Africa, 2016. / Introduction The purpose of this study was to conduct a homoeopathic proving of Withania somnifera in the thirtieth centesimal potency [30CH], thereafter to determine and report the symptomatology in standard materia medica and repertory format, and then compare this symptomatology to the indigenous African and Ayurvedic medicinal usage of Withania somnifera. Methodology The homoeopathic drug proving of Withania somnifera 30CH was conducted as a randomised, double-blind, placebo-controlled trial at the Durban University of Technology. The intervention, Withania somnifera 30CH, was manufactured according to methods 6, 8a and 10 of the German Homoeopathic Pharmacopoeia [GHP] (Driehsen, 2003). Thirty healthy subjects were recruited as provers after meeting with the inclusion criteria (see Appendix A). The provers were randomised into two groups: 80% of provers received verum and 20% received a placebo control identical in appearance to the verum. The vehicle for both verum and control was six lactose powders, self-administered sublingually three times daily by all provers over two consecutive days. The measurement of proving symptoms was two-fold: a subjective account of symptoms produced by the verum/placebo recorded daily in a journal provided, and objective monitoring by the researcher. After the proving-generated symptoms had subsided, journals were collected and data analysis commenced. The accepted symptoms were collated and reported in standard materia medica and repertory format. Results The proving of Withania somnifera 30CH produced a total of 282 rubrics, five of which were newly formulated rubrics. There were 184 Grade 1 rubrics, 98 Grade 2 rubrics and 0 Grade 3 rubrics. The majority of rubrics were represented in the MIND, DREAMS, HEAD and GENERALS sections of the repertory. Analysis of results provided insight into the similarities and differences between indigenous African and Ayurvedic medicinal usage of Withania somnifera and the relationship to other homoeopathic medicines. Conclusion Clearly observable symptomatology was produced by healthy provers in response to the administration of Withania somnifera 30CH. In addition, there was significant correlation between the proving symptomatology and the indigenous African and Ayurvedic medicinal usage of Withania somnifera. / M

published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy