The aim of this thesis was to find optimal anticancer regimen of therapy, based on a naturally occuring polysaccharide laminarin. It was studied on the Mus musculus skin melanoma B16-F10. There were explored an ajuvant effect of LTA. Mixture of laminarin with LPS was applied with various anchorings and was tryed out the possible replacement of LPS. Using the flow cytometry there were determined progress of immune cells during application. This therapy resulted in reduction of tumor volume and the prolongation of overall survival.