Anaerobic digestion of wastewater sludge has widely been in application for stabilization of sludge. With the increase in hauling cost and many environmental and health concerns regarding land application of biosolids, digestion processes generating minimized sludge with better effluent characteristics is becoming important for many public and wastewater utilities. This study was designed to investigate the performance of anaerobic-aerobic-anaerobic digestion of sludge and compare it to anaerobic-aerobic digestion and single stage mesophilic digestion of sludge. Experiments were carried out in three stages: Single-stage mesophilic anaerobic digestion (MAD) 20d SRT; Sequential Anaerobic/Aerobic digestion (Ana/Aer); and Anaerobic/Aerobic/Anaerobic digestion (An/Aer/An). The Anaerobic/Aerobic/Anaerobic digestion of sludge was studied with two options to determine the best option in terms of effluent characteristics. The two sludge withdrawal options were to withdraw effluent from the anaerobic digester (An/Aer/An – A) or withdraw effluent from the aerobic digester (An/Aer/An – B). Different operational parameters, such as COD removal, VS destruction, biogas production, Nitrogen removal, odor removal and dewatering properties of the resulting biosolids were studied and the results were compared among different processes. From the study, it was found that An/Aer/An – B (wastage from aerobic reactor) provided better effluent characteristics than An/Aer/An – A (wastage from anaerobic reactor), Ana/Aer or conventional MAD. The study also shows that the Anaerobic/Aerobic/Anaerobic (An/Aer/An, with wastage from the aerobic or anaerobic digester) digestion of the sludge can improve the biosolids quality by improving the dewatering capabilities, with lower optimum polymer dose, reduced CST and increased cake solid concentration, and reduce the odor generation from the biosolids. Both An/Aer/Ana – A and An/Aer/An – B gave 70% VS removal, compared to 50% with single MAD and 62% with only Ana/Aer. COD removal of both An/Aer/An – A and An/Aer/An – B was 70%, while it was 50% and 66% for single MAD and Ana/Aer respectively. In the aerobic reactors of Ana/Aer and An/Aer/An - B, nitrification and denitrification with removal of nitrogen was observed. The An/Aer/An – B system had more ammonia and TKN removal (70%) than Ana/Aer (64%). The effluent from each stage was analyzed for dewatering ability, cake solid concentration and odor production potential. Compared with a single Ana/Aer system, the extra anaerobic step in An/Aer/An – A and – B reduced polysaccharides in the effluent. The Ana/Aer system released less protein than the conventional MAD system and the addition of the second anaerobic step - especially with system An/Aer/An – B (discharge from aerobic reactor) - greatly reduced protein, resulting in improved dewaterability and less polymer demand. An/Aer/An (both of the options: A and B) had lower CST than single MAD (both 15d and 20d SRT) and Ana/Aer. Compared to Ana/Aer, a reduction of 52% for An/Aer/An – A and 20% for An/Aer/An – B in polymer dose requirement was observed, indicating improved dewatering characteristics. The An/Aer/An – B has higher biosolid cake concentration than MAD or Ana/Aer. The results showed that An/Aer/An (both options: A and B) biosolid had lower odor generation potential than single MAD (15d and 20d SRT) or Ana/Aer. Of all the stages,the An/Aer/An – A and – B system, generated odor which peaked at shorter time and lasted for shorter duration of time. / Master of Science

Addition of iron to sewer lines for chemical phosphorus removal is widely practiced around the world. However, high dosage of iron may prove detrimental to the anaerobic digestion process and also lead to higher organic sulfur odors and deteriorating biosolids quality. The following research focuses on finding an alternative to the use of iron in wastewater systems by comparing the roles of iron and magnesium on mesophilic anaerobic digestion, the digested effluent characteristics and odors in biosolids. Three anaerobic digesters were operated, one serving as a control with no additives, and the other two having known doses of iron and magnesium added. Comparison of the effluent characteristics revealed an improvement in the overall performance of the magnesium amended digester (in terms of pH, solids and COD reduction, alkalinity and gas production) over the other two reactors, suggesting the benefits of magnesium addition. Both iron and magnesium were found to be effective in achieving high levels of phosphate removals and reducing nuisance odors in dewatered sludge cakes. / Master of Science

Sludge digestion has gained importance in recent year because of increasing interest in energy recovery and public concern over the safety of land applied biosolids. Many new alternatives are being researched for reducing excess sludge production and for more energy production. With an increase in solids destruction, the nutrients that are contained in sludge especially nitrogen, are released to solution and can be recycled as part of filtrate or centrate stream. Nitrogen has gained importance because it has adverse effects on ecosystem's as well as human health. NH₄⁺, NO₂⁻, NO₃⁻-, and organic nitrogen are the different forms of nitrogen found in wastewater. While ammonia is toxic to aquatic life, any form of nitrogen can be utilized by cyanobacteria and result in eutrophication. NO₂/NO₃, if consumed by infants through water, can affect the oxygen uptake capability. Hence, removal of nitrogen from wastewater stream before discharging is important. The main purpose of this study was to evaluate the performance of the Cambi process, a thermophylic hydrolysis process used as a pre-treatment step prior to anaerobic digestion. Thermal hydrolysis, as a pre-treatment to anaerobic digestion increases the biological degradation of organic volatile solids and biogas production. The thermal hydrolysis process destroys pathogens and hydrolysis makes the sludge readily available for digestion, while at the same time facilitating a higher degree of separation of solid and liquid phases after digestion. Experiments were conducted in three phases for anaerobic digestion using the Cambi process as pre-treatment. The phases of study includes comparison of two temperatures for thermal hydrolysis (Cambi 150°C and Cambi 170°C), comparison of two solid retention times in anaerobic digestion (15 Day and 20 Day) and comparison of two mesophilic temperatures in anaerobic digestion (37°C and 42°C). Different experimental analyses were conducted for each phase, such as pH, bio-gas production, COD removal, VS destruction, nitrogen removal, odor and dewatering characteristics and the results are compared among all the phases. The second part of the study deals with aerobic digestion of anaerobically digested sludge for effective nitrogen removal and additional VS destruction, COD removal. An aerobic digester is operated downstream to anaerobic digester and is operated with aerobic/anoxic phase for nitrification and de-nitrification. The aerobic/anoxic phases are operated in time cycles which included 40minutes/20minutes, 20minutes/20minutes, full aeration, 10minutes/30minutes, and 12minutes/12minutes. Different time cycles are experimented and aerobic digester is optimized for effective nitrogen removal. 12minutes aerobic and 12minutes anoxic phase gave better nitrogen removal compared to all the cycles. Over all the aerobic digester gave about 92% ammonia removal, 70% VS destruction and 70% COD removal. The oxygen uptake rates (OUR's) in the aerobic digester are measured corresponding to maximum nitrogen removal. The OUR's are found to be close to 60 mg/L during maximum nitrogen removal. The effluent from both anaerobic digester and aerobic digester was collected and analyzed for dewatering capability, cake solids concentration and odor potential. / Master of Science

Bacterial resistance to antibiotics has become a worldwide health problem, resulting in untreatable infections and escalating healthcare costs. Wastewater treatment plants are a critical point of control between anthropogenic sources of pathogens, antibiotic resistant bacteria (ARBs), antibiotic resistance genes (ARGs), and the environment through discharge of treated effluent and land application of biosolids. Recent studies observing an apparent resuscitation of pathogens and pathogen indicators and the widening realization of the importance of addressing environmental reservoirs of ARGs all lead toward the need for improved understanding of ARG fate and pathogen inactivation kinetics and mechanisms in sludge stabilization technologies. This research has investigated the fate of two pathogens, methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli, and various ARGs under pasteurization, anaerobic digestion, biosolids storage, and land application conditions. Pathogen die-off occurs at a rate specific to each pathogen and matrix in ambient and mesophilic temperature environments. Viable but nonculturable (VBNC) states are initiated by thermal treatments, such as thermophilic digestion and possibly pasteurization, and allow the persistence of pathogen cells and any ARGs contained therein through treatment and into the receiving environment where resuscitation or transformation could occur. Raw sludge ARG content does affect digester effluent quality, although the predominant mechanisms of ARG persistence may be different in mesophilic versus thermophilic digestion. In both thermophilic and mesophilic digestion, a correlation was observed between raw sludge and digester ARGs associated with Class 1 integrons, possibly as a result of horizontal gene transfer. ARB survival was shown to contribute to ARG content in mesophilic digestion, but not thermophilic digestion. Thermophilic digestion may achieve a higher ARG reduction because of reduced microbial diversity compared to mesophilic digestion. However, it is evident that horizontal gene transfer still does occur, particularly with highly mobile integrons, so that complete reduction of all ARGs would not be possible with thermophilic digestion alone. Surprisingly, the experiments that introduced various concentrations of antibiotic sulfamethoxazole and antimicrobial nanosilver did not induce enhanced rates of horizontal gene transfer. Finally, ARG concentrations in biosolids increased during cold temperature storage suggesting that there is a stress induction of horizontal gene transfer of integron-associated ARGs. / Ph. D.

Käppala wastewater treatment plant (WWTP) has, during a few years, observed an increase in organic loading rate (OLR) in the mesophilic anaerobic digester R100, due to an increased load to the WWTP. The digestion of primary sludge at Käppala WWTP is today high loaded, with a high organic loading rate (OLR) and low hydraulic retention time (HRT). This study aims to evaluate the effect of the maximum OLR and the minimum HRT for the anaerobic digestion of sewage sludge and to investigate further actions that can be taken into consideration in case of process problems in the digestion. The study consists of (a) a practical laboratory experiment of 6 pilot-scale reactors to investigate how the process stability is affected when the OLR increases and the HRT decreases. (b) A mass balance calculation based on the energy potential in the feeding sludge and the digested sludge. (c) A study of the filterability of the digested sludge. (d) The construction of a forecasting model in Excel, to predict when digester R100 will reach its maximum OLR and minimum HRT. The result of the study shows that the maximum OLR for Käppala conditions is 4.9 g VS dm-3 d-1, meaning that R100 will reach its maximum organic load around the year 2031. An OLR of 4.5-4.9 and an HRT of 12 days is optimal for R100, according to the present study. Keeping the anaerobic digestion process in balance is vital when it comes to the outcome of energy in the anaerobic digestion process. Pushing the process to produce more gas can become counterproductive since a high OLR can lead to process imbalance, which in turn leads to low biogas production. Imbalance in the digestion process can occur fast; therefore, the margin for overload in the anaerobic digestion process must be significant. The methane concentration in the converted biogas and the pH level in the reactor are the best stability parameters for the conditions at Käppala. Ammonia is the less efficient stability parameter since it did not predict or detect any instability during the experimental process. Furthermore, the OLR and HRT have a significant impact on the needed quantity for dewatering polymer. The higher digestion of organic material in the sludge, the bigger the need for the polymer to take care of the rest material.

Käppala wastewater treatment plant (WWTP) has, during a few years, observed an increase in organic loading rate (OLR) in the mesophilic anaerobic digester R100, due to an increased load to the WWTP. The digestion of primary sludge at Käppala WWTP is today high loaded, with a high organic loading rate (OLR) and low hydraulic retention time (HRT). This study aims to evaluate the effect of the maximum OLR and the minimum HRT for the anaerobic digestion of sewage sludge and to investigate further actions that can be taken into consideration in case of process problems in the digestion. The study consists of (a) a practical laboratory experiment of 6 pilot-scale reactors to investigate how the process stability is affected when the OLR increases and the HRT decreases. (b) A mass balance calculation based on the energy potential in the feeding sludge and the digested sludge. (c) A study of the filterability of the digested sludge. (d) The construction of a forecasting model in Excel, to predict when digester R100 will reach its maximum OLR and minimum HRT. The result of the study shows that the maximum OLR for Käppala conditions is 4.9 g VS dm-3 d-1, meaning that R100 will reach its maximum organic load around the year 2031. An OLR of 4.5-4.9 and an HRT of 12 days is optimal for R100, according to the present study. Keeping the anaerobic digestion process in balance is vital when it comes to the outcome of energy in the anaerobic digestion process. Pushing the process to produce more gas can become counterproductive since a high OLR can lead to process imbalance, which in turn leads to low biogas production. Imbalance in the digestion process can occur fast; therefore, the margin for overload in the anaerobic digestion process must be significant. The methane concentration in the converted biogas and the pH level in the reactor are the best stability parameters for the conditions at Käppala. Ammonia is the less efficient stability parameter since it did not predict or detect any instability during the experimental process. Furthermore, the OLR and HRT have a significant impact on the needed quantity for dewatering polymer. The higher digestion of organic material in the sludge, the bigger the need for the polymer to take care of the rest material.