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Generation and characterization of polyclonal antibodies specific to the mouse homeodomain protein HOXB-3Wong, Raymond, 黃偉文 January 1999 (has links)
published_or_final_version / Biochemistry / Master / Master of Philosophy
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Determining the molecular basis of the mutation underlying the mouse neural tube closure mutant, SplotchEpstein, Douglas J. January 1993 (has links)
Splotch (Sp) is a semidominant mouse mutant which maps to the proximal portion of chromosome 1 and is phenotypically expressed as a pleiotropic defect during neurogenesis, resulting in spina bifida, exencephaly and dysgenesis of neural crest cell derivatives. To identify the aberrant gene underlying the defects observed in the Sp mouse mutant we initiated positional cloning strategy. Our preliminary efforts were directed at establishing the boundaries of a deleted chromosomal segment found in the Sp$ sp{r}$ allele, using nine gene probes that were assigned to that region of chromosome 1. Four of these genes, Vil, Des, Inha, and Akp-3, spanning a genetic distance of approximately 15 cM, were found to map within the Sp$ sp{r}$ deletion. In order to further delineate the subchromosomal location of the Sp gene, the proximal segment of mouse chromosome 1 was saturated with microclones isolated from a library of microdissected genomic fragments generated from this region. An additional eight markers were found to map within the confines of the Sp$ sp{r}$ deletion. / During the course of this work a member of the paired box gene family, Pax-3, was described as a candidate for Sp. The striking similarity between the tissue distribution of Pax-3 mRNA in normal developing embryos, and the neural structures affected in Sp mice, together with the chromosome 1 location of Pax-3 led us to examine whether Pax-3 was mutated in three alleles at this locus Sp$ sp{r}$, Sp$ sp{2H}$ and Sp. The entire Pax-3 gene was determined to be deleted in the Sp$ sp{r}$ allele. Analysis of genomic DNA and cDNA clones constructed from RNA isolated from $Sp sp{2H}/Sp sp{2H}$ embryos identified a deletion of 32 nucleotides within the paired type homeobox and is predicted to produce a truncated protein as a result of a newly created termination codon at the deletion breakpoint. The original Sp allele was also characterized and found to contain an A to T transversion at position -2 in the third intron of Pax-3 which abrogates the normal splicing of this intron due to the loss of its natural 3$ sp prime$ splice acceptor. Taken together, these studies indicate that the severe defect in neural tube formation detected in Sp and its allelic variants is linked to the inactivation of the paired box gene Pax-3, and provides direct genetic evidence of a key role for Pax-3 in normal neural development.
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Genetic linkage studies of the splotch neural tube defect gene on mouse chromosome 1Mancino, Franca January 1992 (has links)
Genetic linkage studies of the spontaneously arising splotch allele, Sp, were conducted to identify closely linked molecular markers as a preliminary step for the isolation of the mutant gene. Restriction fragment length polymorphism and microsatellite size variation analyses were employed to follow the segregation of Sp in relation to eight or ten loci previously assigned to the proximal portion of mouse chromosome 1. Although results from an interspecific ((Sp/+ x Mus spretus)F1-Sp x C57BL/6J) backcross study were inconclusive, a panel of 125 intraspecific ((Sp/+ x CBA/J)F1-Sp x CBA/J) backcross mice positioned the Sp gene 0.8 $ pm$ 0.8 centiMorgans distal to the Vil/Des/Inha loci and detected no recombinant between the mutant allele and the murine paired box gene, Pax-3, positioning this locus within 2.9 centiMorgans of Sp (95% confidence limits). Concurrent research has identified alterations in Pax-3 in several Sp allelic variants; thus, this study provides additional genetic evidence in support of the candidacy of Pax-3 for the Sp locus. Effects of genetic background on the penetrance and expression of Sp were also observed.
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Genetic linkage studies of the splotch neural tube defect gene on mouse chromosome 1Mancino, Franca January 1992 (has links)
No description available.
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Neural Tube Defects in the Mouse: Interactions between the Splotch Gene and Retinoic AcidKapron-Brás, C. M. January 1987 (has links)
Note:
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Molecular genetics of Rhabdomys subspecies boundaries : phylogeography of mitochondrial lineages and chromosomal fluorescence in situ hybridizationRambau, Ramugondo Victor 03 1900 (has links)
Thesis (PhD)--University of Stellenbosch, 2003. / ENGLISH ABSTRACT: The geographic genetic population structure and evolutionary history of the African
four-striped mouse, Rhabdomys pumilio, was investigated using mitochondrial
(mtDNA) cytochrome b gene (1140 bp) and control region (994 bp) sequences and a
combination of cytogenetic banding techniques (G- and C-banding), and
fluorescence in situ hybridization. Two cytotypes (2n = 46 and 2n = 48) were
identified by cytogenetic analysis. No evidence of diploid number variation within
populations was found nor were there differences in gross chromosome morphology,
or subtle interchromosomal rearrangements at levels detected by ZOO-FISH. The
comparative painting data (using the complete suite, N = 20, of Mus musculus
chromosome specific painting probes) show that 10 mouse chromosomes have been
retained as chromosomal arms, or intact chromosome blocks within the R. pumilio
genome, six produced double signals, while the remaining four hybridized to three or
more R. pumilio chromosomes. In total, the 20 mouse chromosome paints detected
40 segments of conserved synteny. Their analysis revealed eight R. pumilio specific
contiguous segment associations, a further two that were shared by R. pumilio and
other rodents for which comparable data are available, the Black (Rattus rattus) and
Norwegian (Rattus nONegicus) rats, but not by the Chinese hamster, Cricetulus
grise us. The results suggest that mouse chromosomes 1, 10, and 17 have
undergone extensive rearrangements during genome evolution in the murids and
may be useful markers for enhancing our understanding of the mode and tempo of
chromosome evolution in rodents. Following initial studies using control region
sequences, the phylogeographic appraisal of R. pumilio was done using cytochrome
b gene sequences. Analyses based on a variety of analytical procedures resulted in
the detection of two major mtDNA lineages that correspond roughly to the xeric and
mesic biotic zones of southern Africa. One clade comprises specimens with 2n = 48,
and the other representatives of two cytotypes (2n = 48 and 2n = 46). The mean
sequence divergence (12.0%, range 8.3% -15.6%) separating the two mtDNA clades
is comparable to among-species variation within murid genera suggesting their
recognition as distinct species, the prior names for which would be R. dilecfus and R.
pumilio. Low sequence divergences and the diploid number dichotomy within the
mesic lineage support the recognition of two subspecies corresponding to R. d.
dilecfus (2n = 46) and R. d. chakae (2n = 48). The data do not support subspecific
division within the nominate, R. pumilio. Molecular dating places cladogenesis of the
two putative species at less than 5 million years, a period characterised by extensive
climatic oscillations which are thought to have resulted in habitat fragmentation
throughout much of the species' range. / AFRIKAANSE OPSOMMING: Die geografiesebevolkingsstruktuur en evolusionêre verwantskappe binne die Afrika
streepmuis, Rhabdoys pumilio, is ondersoek deur middel van mitochondriale ONS
volgordebepaling van die geenfragment sitochroom b (1140 basispare) en die
reguleerstreek (994 bp) in kombinasie met sitogenetiese tegnieke (G- en Cbandkleuring
en f1uoreseerende in situ hibridisasie). Twee sitotipes (2n = 46 en 2n =
48) is geidentifiseer deur sitogenetiese analasie. Geen bewys van variasie in die 2n
chromosoomgetal binne bevolkings is gevind nie. Verder is daar ook geen verskil in
die morfologies struktuur van chromosome aanwesig binne bevolkings nie.
Vergelykende data (verkry met behulp van die N = 20 Mus musculus
chromosoomspesifiekepeilers) dui daarop dat 10 muis chromosome behoud gebly
het as chromosoomarms of chromosoomblokke binne die R. pumilio genoom. Ses
peilers het dubbel seine gelewer terwyl die oorblywende vier peilers gehibridiseer het
aan drie of meer R. pumilio chromosome. In totaal het die 20
muischromosoomverwe 40 konserwatiewe segmente geidentifiseer. Die analise dui
agt R. pumilio spesifieke aaneenlopende segmentassosiasies aan, met 'n
addisionele twee wat deur R. pumilio en ander muisagtiges vir wie vergelykende data
beskikbaar is, byvoorbeeld die swart (Rattus rattus) en Noorweegse (R. norvegicus)
rot maar nie die Chinese hamster, Cricetulus grise us, gedeel word. Die resultate stel
voor dat muischromosoom 1, 10 en 17 ekstensiewe herrangskikkings ondergaan het
gedurende die genoom evolusie binne die Muridae en dat hulle waarskynlik
waardevolle merkers kan wees om beide die patroon en tempo van chromosome
evolusie in muisagtiges verder te kan verstaan. Die filogeografiese verwantskappe
binne R. pumilio is ondersoek deur middel van ONS volgordebepalings van die
reguleerstreek asook sitochroom b. Die resultate van hierdie studie het twee
divergente mitochondriale ONS eenhede ontdek wat gekorreleer kan word met
xeriese en mesiese klimaatsones binne suidelike Afrika. Een groep bestaan uit diere
met 2n = 48, terwyl die ander genetiese groep twee sitotipes (2n = 46 en 2n= 48)
insluit. 'n Gemiddelde genetiese divergensie van 12.0% (varieer tussen 8.3% -
15.5%) verdeel die twee mtDNS-groepe en is vergelykbaar met tussenspesievariasie
binne ander muisagtige genera, wat moontlik daarop dui dat twee verskillende
spesies teenwoordig is; die voorgestelde name is R. di/ectus en R. pumilio. Lae
genetiese divergensie binne die mesiese groep versterk die moontlike
teenwoordigheid van twee subspesies, R. d. di/ectus (2n = 46) en R. d. chakae (2n =
48). Die data verleen egter nie steun aan die divisie binne R. pumilio nie.
Molekulêre datering van die twee spesies dui daarop dat die divergensie ten minste 5
miljoen jaar gelede plaasgevind het. Die periode was gekarakteriseer deur ekstensiewe klimaatsossilasies, wat gely het tot habitat fragmentasie in die spesie se
verspreidingsgebied.
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Studying the roles of mouse Sox10 by conditional gene targetingTsang, Wai-hung., 曾偉雄. January 2003 (has links)
published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy
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The Ret gene in the enteric nervous system: expression analysis and generation of ret deficient miceLee, King-yiu., 李景耀. January 2004 (has links)
published_or_final_version / abstract / Surgery / Doctoral / Doctor of Philosophy
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Application of transgenic mice models in functional study of two putative oncogenes: ALC-1 and EIF5A2Chen, Muhan., 陳牧唅. January 2007 (has links)
published_or_final_version / abstract / Clinical Oncology / Doctoral / Doctor of Philosophy
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Generation of transgenic and knockout constructs to study the role of endothelin-1 expression on the development of craniofacial and cardiacstructures趙弘, Chiu, Wun, Kelvin. January 2002 (has links)
published_or_final_version / Molecular Biology / Master / Master of Philosophy
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